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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag

Basic information

species Arabidopsis thaliana
transcript AT3G08530.1
intron # 24
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: LOC_Os11g01380.1 (Oryza sativa), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatac------ctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || |||||||| || |||||||||||||||||||||||||| |||||||| ||||| |||||| | || |||||||||||||| || |||||  | |||||  |||          | ||    || |  |||  | |  |  |||| || ||    |||   | |  ||  ||| || | |  | | |   ||                                 
GGCTAACAGTGCTAAAACATGGAAGGAAGTCTGCTTTGCTTGTGTTGATGCCGAGGAGTTCCGGCTAGCACAAATATGTGGTCTCAATATTATTGTCCAGgtatatatacaggagcaacctgttagaactaaaacatatttcagtacaagtctt----ttcttatctagattttttctaacacccttttttgtatag-------------------------------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: LOC_Os12g01390.1 (Oryza sativa), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatac------ctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || |||||||| || |||||||||||||||||||||||||| |||||||| ||||| |||||| | || |||||||||||||| || |||||  | |||||  |||       |  | ||    || |  |||  | |  |  |||| || ||    |||   | |  ||  ||| || | |  | | | |  | ||                              
GGCTAACAGTGCTAAAACATGGAAGGAAGTCTGCTTTGCTTGTGTTGATGCCGAGGAGTTCCGGCTAGCACAAATATGTGGTCTCAATATTATTGTCCAGgtatatatacaggaccaacctgttagaactaaaacatatttcagtacaagtctt----ttcttatctagattttttctaacacccttttttttgtatag-----------------------------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GRMZM2G325285_T01 (Zea mays), 5'ss of exon 14
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || |||||||| || ||||||||||| |||||||||||||| ||||| || ||||| |||||  | || ||||| |||||||| || ||||| ||||||||  | |  |      ||  | |  || | |||    ||  ||  | |   ||  |   | |  |   | |        | | | |   | || ||    ||  | ||||  || |||    
GGCTAACAGTGCCAAAACATGGAAGGAGGTCTGCTTTGCTTGTGTTGACGCCGAGGAGTTCCGTCTTGCACAAATTTGTGGTCTCAATATTATTATTCAGgtatacaagtaggagcacata-cttaataattttt--ttgtagtatctgctttatagcagagcttctatcactatgatctatttttttctcatatctgcttttgtatttt--tgtttag---

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GRMZM2G057576_T02 (Zea mays), 5'ss of exon 22
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || |||||||| || ||||||||||| |||||||||||||| ||||| || ||||| |||||  | || |||||||||||||| || |||||  |||||||  | |  |       |  |    |  | ||| ||||     || || | |||  ||||   |   | |||      || | |||   | |  | |  | |  | ||||  || |||    
GGCTAACAGTGCTAAAACATGGAAGGAGGTCTGCTTTGCTTGTGTTGACGCGGAGGAGTTCCGTCTTGCACAAATATGTGGTCTCAATATTATTGTTCAGgtatacaagtaggagggcatacttgatgatttttccct----agtctttgtttt--attgcaaagtttctatgat---atatttctttctgacatacctgctcttttttt--tgtttag---

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GRMZM2G111355_T02 (Zea mays), 5'ss of exon 5
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctc---atttatttcctggttaatcttttggtgcttaatag-------------------------------------------------------
 || |||||||| || ||||||||| | |||||||||||||| ||||| || ||||| |||||  | || ||||| |||||||| || ||||| ||||||||  | |  |      ||  | |  || | |||    |   |  |  |  ||  ||    |     |  |||   | | | | |      || |  ||     ||  | ||| |||   | ||                                                         
GGCTAACAGTGCCAAAACATGGAAGAAGGTCTGCTTTGCTTGTGTTGACGCCGAGGAGTTCCGTCTTGCACAAATTTGTGGTCTCAATATTATTATTCAGgtatacaagtaggagcacata-cttaataattttttgtagtatctgctttatagcagagcttctatcactatgatctatttttttctcatatctgcttttgtatttttgtttaggtcgatgatgccttccttacaaagtaaatggtcctctccttgcccccagttatgatctgtttatag

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GRMZM5G822591_T01 (Zea mays), 5'ss of exon 1
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || |||||||| || ||||||||||| |||||||||||||| ||||| || ||||| |||||  | || ||||| |||||||| || |||||  |||||||  | |  |      ||  |    | || |||    ||  ||  | |   ||  |   | |  |   | |  |    || ||| |  ||| | || |   |  | || |  |  |   |||
GGCTAACAGTGCCAAAACATGGAAGGAGGTCTGCTTTGCTTGTGTTGACGCCGAGGAGTTCCGTCTTGCACAAATTTGTGGTCTCAATATTATTGTTCAGgtatacaagtaggagcacatacttgataatttt----ttgtagtatctgctttatagcagagcttctatcactgt--gatctatttttttt-tctcatatctgcttttgtatttttgtttag

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GRMZM2G395549_T01 (Zea mays), 5'ss of exon 9
------------------------------------------------------------------------AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
                                                                         || |||||||| || || |||||||| |||||||||||||| ||||| |  ||||| |||||  | || |||||||||||||| || |||||  ||||| |  | |  |      |  |   ||   | ||| ||||      | || | |||  ||||   |   | |||      || ||| || ||| |  | | | |  ||||||  || |||    
AAACTGGGCTAAGCTGGCTGTTACCCTGGTTAAGCTGAAGCAATACCAAGGTGCTGTGGATGCTGCTCGCAAGGCTAACAGTGCTAAAACGTGGAAGGAGgtctgctttgcttgtgttgacgtggaggagttccgtcttgcacaaatatgtggtctcaatattattgttcagatatacaagtagg---agggcatacttgatttttccct----attctttgtttt--attgcaaagtttctatg-----atatatttc-tttctgacatagctgctctttttttgtttag---

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GLYMA11G35460.1 (Glycine max), 5'ss of exon 23
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
||| ||||||||||| |||||||||||||| |||||||| || ||||| || ||||| ||||| ||||| || |||||||| ||||||||||| |||||||| ||||  |    ||  ||    | ||   |||  ||   |  ||||  | |  | | |  | || |||         | ||| |||  |||   ||| || |   || |    |      
AGCTAACAGTGCAAAAACATGGAAGGAAGTTTGCTTTGCATGTGTTGACGCAGAGGAGTTCCGTTTGGCCCAGATATGTGGGCTTAACATTATTATTCAGgt-aatatgtttgtttgttg----ccaagtcttcaact---aaattttgaaatgaactggaatttgtttttc-------tttat-tcagctatcatctgatttacaattagaaagt------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GLYMA14G06340.1 (Glycine max), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
||| |||||  ||||||||||||||||||| ||||||||||| |||||||| ||||| || || ||||| ||||| ||||| || |||||||| |||||||| |     |||| | | |      |  |  |           ||         ||   |||  || | ||||    || || | || || |||||      |  |                
AGCCAACAGCTCAAAGACATGGAAGGAAGTTTGCTTTGCTTGTGTTGATGCAGAGGAGTTTCGTTTGGCCCAAATTTGTGGACTAAACATTATTATTCAGgtaatatgttgatttcagttttttgatgaact-------gtgtgtgcccatgcacacgcgtgcgtg-tatatataggaacgtttttc-ttcatttctgatgcagaccga-------------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GLYMA18G02960.1 (Glycine max), 5'ss of exon 23
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtga-atacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
||| ||||||||||| |||||||||||||| |||||||| || ||||| || ||||| || || ||||| || |||||||| ||||| ||||| |||||||| | ||      | || |  ||      | |      | | | |   | || ||   || |     ||||||    ||     | |||| |  || |  ||||     ||            
AGCTAACAGTGCAAAAACATGGAAGGAAGTTTGCTTTGCATGTGTTGACGCGGAGGAGTTTCGTTTGGCCCAGATATGTGGGCTTAATATTATTATTCAGgtaatatgttgttttttgcctagtctttgactaaattttgaaatgaactggaatttgttttt-----ctttat---tcagc---tgtcatctgatttccagttagaaagtt------------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: GLYMA02G42560.1 (Glycine max), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
||| |||||| ||||||||||||||||||| ||||||||||| |||||||| ||||| || || ||||| || || ||||| || ||||||||  ||||||| |     |||| | | |             |    | |     | |   | |   | || |||  | ||||          |||   ||| || ||| |          || |       
AGCTAACAGTTCAAAGACATGGAAGGAAGTTTGCTTTGCTTGTGTTGATGCGGAGGAGTTTCGTTTGGCCCAGATTTGTGGACTCAACATTATTGTTCAGgtaatatgttgatttcagt-------------ttttttgatgaactgtgtgtgtgtgt-gtatata-tatatatataggaacgtctttcttaatttctgatgccagaccgagttc-------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: Vv13s0067g01290.t01 (Vitis vinifera), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatctt-----actgagcacaaaagtttccccttacag----------gttttagatttcaattgtgtatg--ctttatagcccaatgtat---ctcatttatttcctggt-taatcttttggtgc-ttaatag-----------
 || |||||  |||||||||||||||| || ||||||||||| |||||||| || || ||||| || ||||| ||||||||||| |||||||| || ||||| |     || | ||        | |    |   |  |  | || |           |||| | | ||   || ||||||  |||   |||| ||||| |   ||  ||||||  | ||| |||| |  | |||  || ||             
GGCTAACAGCTCAAAGACATGGAAGGAGGTTTGCTTTGCTTGTGTTGATGCAGAAGAGTTCCGCTTAGCTCAGATATGTGGTCTCAACATTATTATACAGgtaatgtattgttttttaagggtagggtgggatgataacatcataaaaatggattgttgtttcaaaatttccttctgtatgagcttccaagcctaatgttttcgcttttttattatcaggtctaatttcctagtgtattgatttgaattttgtag

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: PP1S270_53V6.1 (Physcomitrella patens), 5'ss of exon 25
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatc----ttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggt-taatcttttggtgcttaatag--
|||||| ||  | || ||||||| |||||| || ||||||||||| ||| | |||||||| |||||||||||||| ||||| ||||| || || ||||||||     | |  ||    | |   | || | |   ||      |       | ||| ||    |  |   |   || ||| | |  || |      ||   ||   |   ||||| |||  |       
AGCAAATAGCTCTAAAACATGGAGGGAAGTTTGTTTTGCTTGCGTGGATACCGAGGAATTTCGGTTGGCTCAAATTTGTGGCCTTAAGATCATTATTCAGgtattggcttagtcaacataatgtatcatacagaatttaatggatgaaacct-gatatcttcaggatcaacaccat-gcctagttcatttttgaatttgaatgaaatgggctttttgtggctgtggaag

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: PP1S147_10V6.1 (Physcomitrella patens), 5'ss of exon 12
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || || || |||  ||||||||||||||||||||||||||| || || || || || |||||  |||||||||| |||||||| ||  ||||  | |||||    |   | |  | |||          || |   || ||    ||||   |||  ||  | | ||    | ||  |||  | |||||  | || |   |  |||  | || || ||   
GGCCAATAGCGCACGGACATGGAAGGAAGTCTGCTTTGCTTGTGTGGACGCCGAAGAGTTCCGCCTGGCTCAAATTTGTGGTCTCAATGTTATTGTCCAGgtatggataggtttctgctg----------ttacattttgcaa---ttag-cctca--tgattttactacgcaacc--atgcttttcattactatcata--tgttctaatcgtactgaag--

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: PP1S7_102V6.1 (Physcomitrella patens), 5'ss of exon 12
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag--
 || |||||||||  ||| ||||||||||||||||||||||| || ||||| || || || ||  |||||||||| |||||||| ||  ||||  | |||||    |    | | | ||       || | |||  | | |     | || ||||  ||| || | || | ||   ||| ||       |  || | || ||| | | ||| | | | ||    
GGCCAACAGTGCACGGACGTGGAAGGAAGTCTGCTTTGCTTGTGTGGATGCCGAAGAGTTTCGTCTGGCTCAAATTTGTGGTCTCAATGTTATTGTCCAGgtatgaa---aagccttct-------aatgcttcgacatccga---tcagttttc--ttgagttttctgttta--tcaaggt-------tctttccttgattagtttgttgtttcatgatgtgc

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: PP1S452_30V6.1 (Physcomitrella patens), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatctt---actgagcacaaaagtttccccttacaggttttagatt-tcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || || |   ||  ||| ||||||||||| || || ||||| ||||||||||||||||| || || || ||||| || ||||||||  | ||  | ||||||| |   | || ||   ||  ||    | |   |  | |    |  |     |  |||  |||||| |    | |    |    ||   | |||| |                           
GGCCAATAACTCACGGACTTGGAAGGAAGTGTGTTTCGCTTGTGTTGATGCTGAGGAATTTCGATTAGCGCAAATCTGCGGTCTTAATGTCATTGTCCAGgtgattttattatttttcaaccaagtcttagatgattacttagttgactacctttaatcagctgtgtaaggagaagaaaaaatctgatgcaagttatctg--------------------------

upper sequence: AT3G08530.1 (Arabidopsis thaliana), 5'ss of exon 24
lower sequence: EFJ24508 (Selaginella moellendorffii), 5'ss of exon 24
AGCAAACAGTGCAAAGACATGGAAGGAAGTCTGCTTTGCTTGCGTTGATGCTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacctgatcttactgagcacaaaagtttccccttacaggttttagatttcaattgtgtatgctttatagcccaatgtatctcatttatttcctggttaatcttttggtgcttaatag
 || |||||||| ||||| ||||| ||||| ||||| ||||| ||||| || || || ||| || | || |||||||||||||| ||||| ||  | ||||| |     |  | |  |||   | |    | |  | ||    | | ||   |   |||   |                                                           
GGCCAACAGTGCGAAGACGTGGAAAGAAGTTTGCTTCGCTTGTGTTGACGCGGAAGAGTTCAGGCTCGCACAAATATGTGGTCTCAACATCATTGTCCAGgtaaggctatagttt--ctggttatac---tctatctttggttggtctaatctgttattaaatccag-------------------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|116468290|gb|EG510882.1|EG510882
EST:     CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAG                         GTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
genomic: CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacct ... tgcttaatagGTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
EST: gi|116435883|gb|EG478475.1|EG478475
EST:     CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAG                         GTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
genomic: CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacct ... tgcttaatagGTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
EST: gi|116431737|gb|EG474329.1|EG474329
EST:     CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAG                         GTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
genomic: CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacct ... tgcttaatagGTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
EST: gi|86058163|gb|DR353920.1|DR353920
EST:     CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAG                         GTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC
genomic: CTGAGGAATTCCGGTTGGCTCAAATATGTGGTCTTAACATTATCATTCAGgtgaatacct ... tgcttaatagGTGGATGACCTGGAAGAAGTCAGCGAGTACTACCAGAACAGAGGGTGCTTC