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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagtgatcttaccctgttttatttatggccatatctatcttattcactatcacacctatgtatttgtgtttgatcttgtggatacagagatattgattcattagcttctcaaatttatccctccattatccaaggagtagcaatactactttcccattccttagattccaactgattttgattctggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG

Basic information

species Zea mays
transcript GRMZM5G886952_T01
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G886952_T01 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os03g16010.1 (Oryza sativa), 3'ss of exon 8
gtgagtgatcttaccctgttttatttatggccatatctatcttattcactatcacacctatgtatttgtgtttgatcttgtggatacagagatattgattcattagcttctcaaatttatccctccattatccaaggagtagcaatactactttcccattccttagattccaactgattttgattctggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG
|||||| ||||| | || | || |||||         | ||| |||||              ||| | | ||||| |  || ||    | ||| |    | ||    | ||    |||| | || ||| || |        || | ||||| | | || |  | | ||  ||  ||||||||  | | | |||| |||||  | || |||| ||||||||||||||||||||| |||| ||||||||||||||||||||| ||| |||||||||| |  | | |||||||  |||
gtgagttatctt-ctctatgttgtttatt------cttgtctaattca-------------atatgtatatttgagcaagtagagtgggggatgtcatgtgatagagtgcttttgtttacctcttcatgatgc--------gctacactacat-cacacttgtaatatatcatatgattttgcatgttgcaatgcagCTTCATCGGGAACCGTGGTTTATGTGGGAAGCAGATTAACTCGGTGTGCAAAGATGCACTTCAGTCACCATCAAATGGCCCTCTGCCACCTTCCGCAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60356786|gb|DN223759.1|DN223759
EST:     ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATC                         CTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
genomic: ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
EST: gi|31360868|gb|CD445225.1|CD445225
EST:     ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATC                         CTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
genomic: ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
EST: gi|32825445|gb|CD965167.1|CD965167
EST:     ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATC                         CTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
genomic: ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
EST: gi|31349376|gb|CD433733.1|CD433733
EST:     ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATC                         CTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
genomic: ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
EST: gi|32807885|gb|CD960119.1|CD960119
EST:     ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATC                         CTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA
genomic: ACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 302

CAATGTTTCCATGAACTTCCTGACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 190

CAATGTTTCCATGAACTTCCTGACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG


Block sizes: 49 (upstream exon), 44 (downstream exon)
Score: 72

CAATGTTTCCATGAACTTCCTGACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 200

CAATGTTTCCATGAACTTCCTGACAGGAGCCATACCATCTTCTGGTTCACTTGTCAATTTCAATGAGACATCgtgagtgatc ... ggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

caatactactttcccattccttagattccaactgattttgattctggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG
                                  attttgatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagtgatcttaccctgttttatttatggccatatctatcttattcactatcacacctatgtatttgtgtttgatcttgtggatacagagatattgattcattagcttctcaaatttatccctccattatccaaggagtagcaatactactttcccattccttagattccaactgattttgattctggtaatgtagCTTTGTTGGCAACCTTGGTTTATGTGGGAAGCAGATAAACTTGGTGTGCAAAGATGCACTTCAATCATCATCAAATGGTCTGCAGTCACCTTCACCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGCAG