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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagttttataatggctagagtgcactgttcagggcaacaattctagataaagtaacaaatgatccctgaatagcactgaaccatatcagttcaattgcacgcttcttatgtataacagactttactgtgtatagtatggcttttgttctgatgtgtgatgacttgcag

Basic information

species Zea mays
transcript GRMZM5G886952_T01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G886952_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os03g16010.1 (Oryza sativa), 5'ss of exon 5
GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagttttataatggctagagtgcactgttcagggcaacaattctagataaagtaacaa-atgatccctgaatagcactgaaccatatcagttcaattgcacgcttcttatgtataacagactttactgtgtatagtatggcttttgttctgatgtgtgatgacttgcag
 |||||||| ||||| || |||||||| ||||||||||| ||| |||| |||||||||||| | |||||| |||||||||        |||      ||||||           ||| ||     ||||||  |||       || | |  |  |  | ||   | |  ||   |  || | ||  ||   |   |||||  | | |  | || |||          ||| ||||||  ||||
ATCACTGCAAGGGAATAGCTTGTATGGCTCACTCCCTCCAGAATTGGGAAATTGTACCAAGTTACAACAACTgtaagttt--------ctaagtattactgttt----------ttccagcatttgtaacaccatgtcttgcaaaaaactattcataacattcatactgttatgcaattttaattgatgcaactttttacgttttgtgttctgactt----------gtgagatgaccagcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60356786|gb|DN223759.1|DN223759
EST:     TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATT                         GTATCTACAAGGAAATTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
genomic: TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
EST: gi|211487045|gb|FL021472.1|FL021472
EST:     TATGGNTCACTCCCTCCTGAACTGGGGGATTGTACCAAGCTGCCACAATT                         GTATCTACAAG-AAATTATTTTAGTGGGATACATCCC
genomic: TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGG-ATACATCCC
EST: gi|149109166|gb|EE190051.2|EE190051
EST:     TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATT                         GTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
genomic: TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
EST: gi|149087972|gb|EE177200.2|EE177200
EST:     ATGGTTCACTCCCCTC-TGAACTGGGGAATTGTACCAAGCTGCAACAATT                         GTATCTACAAGGAAACTATTTAAGTGGATACATCCCCTCAGAATTTGGCGA
genomic: ATGGTTCACTCCC-TCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
EST: gi|149087580|gb|EE176978.2|EE176978
EST:     TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATT                         GTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAAATTTG-CG
genomic: TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAA-TTTGGCG
EST: gi|31354140|gb|CD438497.1|CD438497
EST:     TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATT                         GTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
genomic: TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
EST: gi|31349376|gb|CD433733.1|CD433733
EST:     TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATT                         GTATCTACAAGGAAATTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA
genomic: TATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 176

GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGATCTGGTGGAACTCGAGGCATT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 94

GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGATCTGGTGGAACTCGAGGCATT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 17

GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGATCTGGTGGAACTCGAGGCATT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 110

GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagtttta ... tgacttgcagGTATCTACAAGGAAACTATTTAAGTGGATACATCCCTTCAGAATTTGGCGATCTGGTGGAACTCGAGGCATT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTCACTGCAGGGGAACAGTTTGTATGGTTCACTCCCTCCTGAACTGGGGAATTGTACCAAGCTGCAACAATTgtaagttttataatggctagagtgcactgttcagggcaacaattctagataaagtaacaaatgatccctgaatagcactgaaccatatcagttcaattgcacgcttcttatgtataacagactttactgtgtatagtatggcttttgttctgatgtgtgatgacttgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC