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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...cccttttgctagtgaaatatgtgtatgttgctaacaagtttacctgtatttttcttgatatttttcgtggtctgaatttctactgttaaaacattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT

Basic information

species Oryza sativa
transcript LOC_Os01g13200.1
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os01g13200.1 (Oryza sativa), 3'ss of exon 3
lower sequence: GRMZM2G123540_T01 (Zea mays), 3'ss of exon 3
cccttttgctagtgaaatatgtgtatgttgctaacaagtttacctgtat----ttttcttgatatttttcgtggtctgaatttctactgttaaaacatt-----ttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT
            |   |||| |  ||||| || ||   | ||  | |      |||| |||||  || |  | |    |  ||||  ||  |  |  ||     ||||| ||||||||||||||||| ||||| ||||||||||||||||| ||||| ||||| |||||||||||||| || || || ||||||||||||||||| |||
---------ggatcctatatctccatgttcctgacttatgtatttctgaagtattttgttgattgttctgttagctaaagcttctgttgcaaccattttataatttcagCCGCATTTGGAATGAGACGTGGCTAGCAGGTTTCTTTTACAAGCCGTGTAATTACGAACTATTTGTCAAGCAATCACTAAACATGGAAATGGCTATTATT

upper sequence: LOC_Os01g13200.1 (Oryza sativa), 3'ss of exon 3
lower sequence: Vv13s0019g01720.t01 (Vitis vinifera), 3'ss of exon 3
cccttttgctagtgaaatatgtgtatgttgctaacaagtttacctgtatttttcttga--tatttttcgtggtctgaatttctactgttaaaacattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT
   ||||     |   ||   | |    ||| || || |||||      || | || |  ||||  ||||| | |   | | |  || |     |||||||| || || ||||||||||| ||| |  || |||||||||||||||| ||||||||||| |||||||| || ||||| || || ||||| ||||||||  | 
-ttttttaagcatattatcattttgaactgcaaa-aattttacagagccttctgtttacttattaatcgtgatttttgtattttgtgctgtggaattttcagCCGTATCTGGAATGAGACATGGTTATCAAGTTTCTTTTACAAACCATGCAATTATGAGCTATTTGTGAAGCAGTCTCTTAATATGGAGATGGCTATTGTC

upper sequence: LOC_Os01g13200.1 (Oryza sativa), 3'ss of exon 3
lower sequence: PP1S142_149V6.1 (Physcomitrella patens), 3'ss of exon 2
cccttttgctagtgaaatatgtgtatgttgctaacaagtttacctgtatttttcttgatatttttcgtg---gtctgaatttctactgttaaaacattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT
   ||| | | ||| | | | ||  ||| | |||  |  ||| |     ||     | | |    || |    |  |||||| |  |||| |  | ||  ||| || || ||||| |||||||||||  | || || || |  ||||||||||| ||||| || |||||||||||||| || |||||||||| ||||||  | 
-gttttggttcgtgtatt-tttggctgtggttaatgaagttatcatcgattgatgagttttccaacgggcgaatgcgaatttatcttgtttat-ccttcgcagCCGTATATGGAACGAGACTTGGCTATCTGGCTTTTTCTGGAAACCGTGCAACTATGAGCTTTTTGTCAAACAGTCTCTCAACATGGAAACGGCTATTGTA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|58602349|gb|CK012877.1|CK012877
EST:     GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAG                         TCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAA
genomic: GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAGgtattgtgat ... acattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAA
EST: gi|58664237|gb|CK052923.1|CK052923
EST:     GTACAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAG                         TCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAA
genomic: GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAGgtattgtgat ... acattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAA
EST: gi|88719317|gb|CI742827.1|CI742827
EST:     GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAG                         TCGCATTTGGAATG
genomic: GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAGgtattgtgat ... acattttcagTCGCATTTGGAATG
EST: gi|58660401|gb|CK049081.1|CK049081
EST:     GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAG                         TCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAA
genomic: GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAGgtattgtgat ... acattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAA
EST: gi|8404394|gb|AU091748.1|AU091748
EST:     GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAG                         TCGCATTTGGAATGAGACTTGGCTGGGCAAGTTTCTTTTACAAACCGTGCA
genomic: GTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAGgtattgtgat ... acattttcagTCGCATTTGGAATGAGACTTGGCTGG-CAGGTTTCTTTTACAAACCGTGCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 47 (upstream exon), 59 (downstream exon)
Score: 87

GGTGTAAAAATCATTTACAGAACTAAAGAATTGAAAGAGAAACAGGACAGAAGgtattgtgat ... acattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtatttttcttgatatttttcgtggtctgaatttctactgttaaaacattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT
       tcttgat  putative branch site (score: 87)
                                              cattttc  putative PPT
     tttcttgatattttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
cccttttgctagtgaaatatgtgtatgttgctaacaagtttacctgtatttttcttgatatttttcgtggtctgaatttctactgttaaaacattttcagTCGCATTTGGAATGAGACTTGGCTGGCAGGTTTCTTTTACAAACCGTGCAATTATGAACTATTTGTCAAACAGTCGCTGAACATGGAAATGGCTATCATT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CATGGA
- cttttgc
- - - - - - - - - - - - -tgttgct
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tactgtt