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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgacgcctcaaccgacgagcgctcatcaccctccactcatcttgccctgtgagtttcgactactgacaagtttgttcctttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG

Basic information

species Zea mays
transcript GRMZM2G052688_T01
intron # 2
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32944789|gb|CF049608.1|CF049608
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
EST: gi|149027659|gb|EE286042.2|EE286042
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
EST: gi|76923847|gb|DV169691.1|DV169691
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
EST: gi|211129939|gb|FL016639.1|FL016639
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
EST: gi|91872021|gb|EB401978.1|EB401978
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
EST: gi|78088729|gb|DV517122.1|DV517122
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
EST: gi|78073936|gb|DV502370.1|DV502370
EST:     AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAG                         CAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA
genomic: AAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 132

CCTGAAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 28

CCTGAAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG


Block sizes: 46 (upstream exon), 47 (downstream exon)
Score: 20

CCTGAAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG


Block sizes: 42 (upstream exon), 47 (downstream exon)
Score: 9

CCTGAAGTTGCTCACCTCGCCCACAACGCTGCCGTGTTACCACACATCCTGCAGgtgacgcctc ... tttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cactcatcttgccctgtgagtttcgactactgacaagtttgttcctttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG
                           tactgac  putative branch site (score: 9)
                                     tttgttcctttctttc  putative PPT
                                             tttcttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgacgcctcaaccgacgagcgctcatcaccctccactcatcttgccctgtgagtttcgactactgacaagtttgttcctttctttcagCAAGTGTGCCCTTACACCGACTTCAAAAGGCTACAGCTGTGCCATTTGCAAATCAGGCTATTGTTCTCAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTG