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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttttgccattaatatgctactttgtctatagtttggctaatctctttctgctaacatccctatattgcttttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG

Basic information

species Zea mays
transcript GRMZM2G158627_T02
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G158627_T02 (Zea mays), 3'ss of exon 8
lower sequence: LOC_Os06g43760.1 (Oryza sativa), 3'ss of exon 21
gttttgccattaatatgctactttgtctatagtttggctaatctctttctgct-------aacatccctatattgcttttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG
  | || ||  |||||| | |  | |   |||||||  |  | ||| |||  |       |  |||  ||  ||  ||||  | |||| || || |||||| |||| ||||| |||||||| || |||||||||||||||||||| |||||||||
-gtatgtca--aatatgtttccatatgagtagtttgtatgctatctgtctattttgtcatattatcattactttttttttcatgtagGTGATGTGTTAGTCGTTTTGGATCTTCGAGCTGATCAATCATTGTTTGAAGCTGGAGTTGCTCGAGAG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71432888|gb|DR813938.1|DR813938
EST:     CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATG                         GCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
genomic: CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
EST: gi|40334151|gb|CK368221.1|CK368221
EST:     CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATG                         GCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
genomic: CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
EST: gi|74240586|gb|DT648500.1|DT648500
EST:     CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATG                         GCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
genomic: CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
EST: gi|45568479|gb|CK986083.1|CK986083
EST:     CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATG                         GCGACGTNTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
genomic: CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
EST: gi|211108629|gb|FL325826.1|FL325826
EST:     CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATG                         GCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG
genomic: CAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 199

GTTGTCCGACAATTCAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 132

GTTGTCCGACAATTCAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 130

GTTGTCCGACAATTCAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 79

GTTGTCCGACAATTCAAGCGTCCTGAAAACGTGTCGGAGAAGGAGATTGATGCTGCAGGAGATGgttttgccat ... tttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tctatagtttggctaatctctttctgctaacatccctatattgcttttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG
                        tgctaac  putative branch site (score: 79)
                                        ttgctttt  putative PPT
                                            ttttgttata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttttgccattaatatgctactttgtctatagtttggctaatctctttctgctaacatccctatattgcttttgttatagGCGACGTTTTAGTCATTTTAGATCTACGAGCTGACCAGTCATTGTTTGAAGCTGGAGTGGCTCGAGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGCTGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGGA