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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagtccagtctcctgctctctaactagacctttttcaattggaagttgaatacatatccctaattcagactcttatgctgttactggttgaatg...

Basic information

species Zea mays
transcript GRMZM2G058498_T01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G058498_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os08g07960.2 (Oryza sativa), 5'ss of exon 5
AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagtccagtctcctgctctctaactagacctttttcaattggaagttgaatacatatcc-ctaattcagactcttatgctgttactggttgaatg
|| ||||||| ||||| |||||||| || |||||||||||| |||| ||||||||||| |||||||||||||| || |||||||||||||||||| ||||||||| ||   |     | |||   ||   ||  |||||||  || |||| | |||| ||||||  | | |  |    || |    |||| | | |     
AGGCTCAGACTGTTGCAATGCATGACGCACCTGTCAAGGAGATCGCTTGGATTCCTCAAATGAATCTTCTTGTCTCTGGAAGCTGGGATAAGACTCTAAGgtgtgaaaaatccaatccttgcagcctcggtattcctttttacat-ggaattcgaattcatatcaaccactctatttactcagatagttattcgctagtaa
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71319015|gb|DR796255.1|DR796255
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|71435207|gb|DR816257.1|DR816257
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|211049997|gb|FL232804.1|FL232804
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAG
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAG
EST: gi|67019316|gb|CO448065.1|CO448065
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|31355022|gb|CD439379.1|CD439379
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|32915973|gb|CF020785.1|CF020785
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|71427496|gb|DR808546.1|DR808546
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|78092605|gb|DV520979.1|DV520979
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|71765073|gb|DR963010.1|DR963010
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|32868065|gb|CF007747.1|CF007747
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|62550552|gb|DN830972.1|DN830972
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|71310174|gb|DR791376.1|DR791376
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTG
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGG-ATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTG
EST: gi|50339684|gb|CO534810.1|CO534810
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
EST: gi|67024188|gb|CO452937.1|CO452937
EST:     ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAG                         GTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA
genomic: ATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 97

AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGAACGTTGCTATGCTCTTACTGTGAATTATCCCCTTATGATTGTTGGAACT


Block sizes: 45 (upstream exon), 47 (downstream exon)
Score: 100

AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGAACGTTGCTATGCTCTTACTGTGAATTATCCCCTTATGATTGTTGGAACT


Block sizes: 44 (upstream exon), 49 (downstream exon)
Score: 85

AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGAACGTTGCTATGCTCTTACTGTGAATTATCCCCTTATGATTGTTGGAACT


Block sizes: 43 (upstream exon), 49 (downstream exon)
Score: 69

AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagt ... ctctttatagGTACTGGGATACAAGGCAATCAAATCCTGTGCATATTCAGCAACTTCCTGAACGTTGCTATGCTCTTACTGTGAATTATCCCCTTATGATTGTTGGAACT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
AGCCTCAGACGGTTGCTATGCATGATGCCCCTGTCAAGGAGGTCGCCTGGATTCCTCAGATGAATCTTCTTGTATCCGGAAGCTGGGATAAGACTTTAAGgtgtgcaagtccagtctcctgctctctaactagacctttttcaattggaagttgaatacatatccctaattcagactcttatgctgttactggttgaatg

- - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGCTG