ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

gtatgtacagagaagctctatttccaagtgctttgctttcattttgttttgctttgttttatgtattaattagttgtttgtattcacttttcacgacttaatgtgttgcaattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACATTGCAAGAACTGCAAGTTCTTGTTGAACGAAAGCAGTTCCTTGAATCCA

Basic information

species	Zea mays
transcript	GRMZM2G374574_T02
intron #	10
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G374574_T02 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os02g01170.1 (Oryza sativa), 3'ss of exon 3

gtatgtacagagaagctctatttccaagtgctttgctttcattttgttttgctttgttttatgtattaat-tagttgtttgtattcacttttcacgacttaatgtgttgcaattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACATTGCAAGAACTGCAAGTTCTTGTTGAACGAAAGCAGTTCCTTGAATCCA
                         ||||| | | || | | |   ||    |  |||||   | ||  |  | ||||||   |  | ||     ||   ||||   || |  || |||||| || || || ||||||||| | ||||||||| |||| || || ||||||||||||||||||||| ||||||||||||| |||  ||||||||||||||
-----------------------gtaagtgatatactcttactggattaatatcagtttttccttttgttacatttgttttattgaagttagtctgaacaaatgaactgtatgtg-ccagGAGCTGGACTTATTTGACATAATCTCATTTGATGCTGAATTCGGAAAGACATTGCAAGAACTGCAAATTCTTGTTGAACGGAAGAGGTTCCTTGAATCCA

upper sequence: GRMZM2G374574_T02 (Zea mays), 3'ss of exon 10
lower sequence: Vv04s0023g03830.t01 (Vitis vinifera), 3'ss of exon 10

gtatgtacagagaagctctatttccaagtgctttgctttca--ttttgttttgctttgttttatgtattaattagttgtttgta-ttcacttt-tcacgacttaatgtgttgcaattggc--cagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACATTGCAAGAACTGCAAGTTCTTGTTGAACGAAAGCAGTTCCTTGAATCCA
           | |   | | ||   ||| || |  | |||  ||||   |  || ||||| |  ||||     ||  |  |   |||| ||  |||  | || |  | ||   ||      ||||| || ||||||  ||||||  | ||||||||| ||||  |||||||||  ||||||||||||||||| ||||||   || || ||||  || ||     
-----------gtaatacaaattt-gagttctatattgtcaaattttacctgcctctgtttaacatatttccctgtcatgggaggttcatttggtcaaaatttcaaat-ttatgatcccttgcagGATCTGGATTTGCATGACATCCTATCATTTGATGCTGAACTTGGGAAGATTTTGCAAGAACTGCAAGTCCTTGTTTGTCGGAAACAGTATCTGGATGGTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|22521271|gb|BU080082.1|BU080082

EST:     TTTATCAACAGCG-TTTATAAGCTTATACTTGGACAG                         GAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA
genomic: TTTATCAACAGCGTTTTATAAGCTTATACTTGGACAGgtatgtacag ... aattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA

EST: gi|26558996|gb|CA831231.1|CA831231

EST:     TTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAG                         GAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA
genomic: TTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAGgtatgtacag ... aattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA

EST: gi|87157724|gb|DY402513.1|DY402513

EST:     TTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAG                         GAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA
genomic: TTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAGgtatgtacag ... aattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA

EST: gi|211367975|gb|FL128470.1|FL128470

EST:     TTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAG                         GAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA
genomic: TTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAGgtatgtacag ... aattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 141

TTTCCGCTTAATAGGTCAAGTGATCGCAAAAGTCCTGCAAGATGGCAGACTTTTAGATTTGCCTTTATCAACAGCGTTTTATAAGCTTATACTTGGACAGgtatgtacag ... aattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACATTGCAAGAACTGCAAGTTCTTGTTGAACGAAAGCAGTTCCTTGAATCCA

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 159

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 108

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 183

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

attaattagttgtttgtattcacttttcacgacttaatgtgttgcaattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACATTGCAAGAACTGCAAGTTCTTGTTGAACGAAAGCAGTTCCTTGAATCCA

            tttgtatt  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtatgtacagagaagctctatttccaagtgctttgctttcattttgttttgctttgttttatgtattaattagttgtttgtattcacttttcacgacttaatgtgttgcaattggccagGAACTTGATTTGTTTGACATAGTGTCATTTGATTCTGAGTTTGGGAAGACATTGCAAGAACTGCAAGTTCTTGTTGAACGAAAGCAGTTCCTTGAATCCA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAC