ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GATCACCAGCGAAGGAGGATGGACCCTGGGACAAATGAGCCATTGTTCACAAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgcttaaattcttggactggttcaagcgcttgctctatctcttccctttactttggttcaagcgcttggccgcttgcgctatctcttttccttttagtttggctatctctgttgatctggaataagtagatattgtgttaacattgtcttttacaatgtgctcttctatttcag

Basic information

species	Zea mays
transcript	GRMZM2G124051_T01
intron #	11
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G124051_T01 (Zea mays), 5'ss of exon 11
lower sequence: LOC_Os03g07080.1 (Oryza sativa), 5'ss of exon 11

GATCACCAGCGAAGGAGGATGGACCCTGGGACAAATGAGCCATTGTTCACAAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgcttaaattcttggactggttcaagcgcttgctctatctcttccctttactttggttcaagcgcttggcc-gcttgcgctatctcttttcc-ttttagtttggctatctctgttgatctggaataagtagatattgtgttaacattgtcttttacaatgtgctcttctatttcag
|| |||||| | ||||| ||||||||||  |||||||| ||| | |||||||||||||| |||||||| || || ||| | |||||||||||||||||||||| ||| | ||| | |       ||| ||  |||  || |   | |   ||  | | ||     |  ||| |     | |  ||  | | |||||| ||| ||                                                                             
GAGCACCAGAGGAGGAGAATGGACCCTGCAACAAATGAACCACTTTTCACAAATTGCACGAGGGATTTCACTGGGACCATTGATTACATATTTTACACAGgtttgtggtgaaacttt-------gttgaaatgctgcctgtgg-tgtgttctggaatatgcaaatattgctagatttacctttgcattgtattttccatttcag-----------------------------------------------------------------------------

upper sequence: GRMZM2G124051_T01 (Zea mays), 5'ss of exon 11
lower sequence: GLYMA09G15980.2 (Glycine max), 5'ss of exon 10

GATCACCAGCGAAGGAGGATGGACCCTGGGACAAATGAGCCATTGTTCACAAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgcttaaattcttggactggttcaagcgcttgctctatctcttccctttactttggttcaagcgcttggccgcttgcgctatctcttttccttttagtttggctatctctgttgatctggaataagtagatattgtgttaacattgtcttttacaatgtgctcttctatttcag
|| || ||   ||||||  ||||   ||| || ||||| || || |||||||||  ||| || |||||||  || || |||||||||||||||||||||||| ||   |    || |   | |     || |      ||| | ||||      | |||  |  |      || |   | |    |                                                                                             
GAGCAGCATAAAAGGAGACTGGATGGTGGAACGAATGAACCTTTATTCACAAATGTCACTAGAGATTTTATTGGCACTCTAGATTACATATTTTACACAGgtgagca-tggtgttatatcattttgataaacataatatctgtatcttgttcacaattttatgaagagatatgacttgtggtttcag--------------------------------------------------------------------------------------------

upper sequence: GRMZM2G124051_T01 (Zea mays), 5'ss of exon 11
lower sequence: GLYMA02G26620.2 (Glycine max), 5'ss of exon 10

GATCACCAGCGAAGGAGGATGGACCCTGGGACAAATGAGCCATTGTTCACAAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgcttaaattcttggactggttcaagcgcttgctctatctcttccctttactttggttcaagcgcttggccgcttgcgctatctcttttccttttagtttggctatctctgttgatctggaataagtagatattgtgttaacattgtcttttacaatgtgctcttctatttcag
|| || ||   |||||| |||||   ||| |||||||| || || |||||||||  ||| || |||||||  || || |||||||||||||||||||||||| ||   |    || |   | |     || |      ||| | ||||      | |||  |  ||     || |   | |    |                                                                                             
GAGCAGCATAAAAGGAGAATGGATGGTGGAACAAATGAACCTTTATTCACAAATGTCACTAGAGATTTTATTGGCACTCTAGATTACATATTTTACACAGgtgagca-tggtgttatatcattttgataaacttaatatctgtatcttggtcacatttttatgaaaagatatgacttgtggtttcag--------------------------------------------------------------------------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|91049453|gb|EB159871.1|EB159871

EST:     AAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAG                         CGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCT
genomic: AAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgctt ... tctatttcagCGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCT

EST: gi|211089863|gb|FL154003.1|FL154003

EST:     AAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAG                         CGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCT
genomic: AAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgctt ... tctatttcagCGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCT

EST: gi|24767403|gb|CA402546.1|CA402546

EST:     AAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAG                         CGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCT
genomic: AAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgctt ... tctatttcagCGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 53

GATCACCAGCGAAGGAGGATGGACCCTGGGACAAATGAGCCATTGTTCACAAATTGCACAAGGGATTTTACCGGTACCCTAGATTACATATTTTACACAGgttagtgctt ... tctatttcagCGGATTCATTGACTGTAGAGTCATTATTGGAGCTGTTGGATGAGGATAGCTTGAGAAAAGACACGGCTCTTCCTTCTCCTGAATGGTCATCGGATCATAT

Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 55

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 91