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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttgctttaacataagtgctttgcgattcctttttgtaatttacttaaattgcgataggcggtttgcttatgatgtgttgaatgaatcttgtttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG

Basic information

species Zea mays
transcript GRMZM2G102231_T01
intron # 3
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G102231_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os08g14580.1 (Oryza sativa), 3'ss of exon 2
ttgctttaacataa-gtgctttgcgatt---cctttttgtaatttacttaaattgcgataggcggtttgcttatgatgtgttgaatgaatcttgtttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG
  |       ||   | ||||    |||   |||    ||  | |  || | ||||  | |    |  |  ||  ||  | | |||||||  | | | |||||||||| ||||||||| | |||||||| ||||| || |||||||||||||||||||| |||||
--gtccagggattgtgagcttccaaattaggcctgagggtggtgtggtttatttgcttttgctcatgagtataatattagat-aatgaattgt-tctgttgcagGAGTGCTACGTTTGATCCATCATCTCCATGCAAATGGAGTACCAATGGCTGTTGCAACAGG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211004136|gb|FL435012.1|FL435012
EST:     CATCCTGTACCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: CATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|211016904|gb|FL048021.1|FL048021
EST:     CCATCCTGTACCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: CCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|14245028|gb|BG842966.2|BG842966
EST:     CCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: CCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|71438331|gb|DR819381.1|DR819381
EST:     GCGCGAGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: GCGCGAGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|50325634|gb|CO520760.1|CO520760
EST:     TCCCATCCTGTACCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: TCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|50338089|gb|CO533215.1|CO533215
EST:     AGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: AGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|44901216|gb|CK827761.1|CK827761
EST:     ATGCTCCAAGCGCTCTTCCCATCGTGTACCAAGCTGCCAG                         GAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
genomic: ATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTG
EST: gi|149013686|gb|EE043136.2|EE043136
EST:     CGCGAGAGCATGGTCCAAGCGCTCTTCCCATTCCTGTACCAAGCTGCCAGG                         GAGGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGC
genomic: CGCGAGAGCATGCTCCAAGCGCTCTTCCCA-TCCTGTACCAAGCTGCCA-Ggtgtgtcatt ... tttcttgcagGA-GTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 122

GACGAGTGCGGCCTCAACGGCCTCCTCACCCCCGAGCAGTTCCTCGAGGAGCGCGAGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 216

GACGAGTGCGGCCTCAACGGCCTCCTCACCCCCGAGCAGTTCCTCGAGGAGCGCGAGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 172

GACGAGTGCGGCCTCAACGGCCTCCTCACCCCCGAGCAGTTCCTCGAGGAGCGCGAGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG


Block sizes: 48 (upstream exon), 46 (downstream exon)
Score: 140

GACGAGTGCGGCCTCAACGGCCTCCTCACCCCCGAGCAGTTCCTCGAGGAGCGCGAGAGCATGCTCCAAGCGCTCTTCCCATCCTGTACCAAGCTGCCAGgtgtgtcatt ... tttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aaattgcgataggcggtttgcttatgatgtgttgaatgaatcttgtttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG
                                        tcttgtttctt  CT-rich tract
                     ttatgat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttgctttaacataagtgctttgcgattcctttttgtaatttacttaaattgcgataggcggtttgcttatgatgtgttgaatgaatcttgtttcttgcagGAGTACTACGTTTGGTGCATCATCTTCATGCCAACGGAGTACCAATGGCTGTTGCGACAGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT