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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtttcttaatttgtagaagttatggaacatatgttatttgtatttatttttacttaagctgtagcagaacgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG

Basic information

species Zea mays
transcript GRMZM2G015132_T01
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G015132_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os02g01500.1 (Oryza sativa), 3'ss of exon 7
gtaagtttcttaatttgtagaagttatggaacatatgttatttgtatttatttttactta-------------------------agctgtagcagaa-cgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG
||||||||  |||| |||| | | ||     ||||| || |||  ||||||||||| |||                         ||||| || |||| | ||||| | ||||| |  |||  || |||  |||| ||||||||||| ||||||||||||||||||||||||||||| |||||||| ||||||||||||||||| || ||||| ||||||||||||
gtaagttttctaatatgta-aggctacatgccatatattgtttagatttatttttagttatgcagttagccagttacttctattaagctgcagtagaagctaaatatcctttgaatgcattcctgctgactagGATAAAGCCACTGTAGAGATGGAGTGCATGGAAGAGGGCTATCTCGCTAAGATCATTCATGGAGATGGTGCCAAAGAGATCAAAGTTGGTGAG

upper sequence: GRMZM2G015132_T01 (Zea mays), 3'ss of exon 6
lower sequence: LOC_Os07g22720.2 (Oryza sativa), 3'ss of exon 7
-----------------------gtaagtttcttaatttgtagaagttatggaacatatgttatttgtatttatttttacttaagctgtagcagaa-cgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG
                          | | ||||| || |||    || |      |     |||   ||||  ||||| |||||||| |  |||| | ||||| | ||||| |  |||| || |||  |||| |||||||| || || ||||||||||||||||||||||||||||||||||| ||||||||||||||| | || ||||| ||||||||||||
gtaagttttctgatctctaaggcagcaatatcttagttagtacttatttttagtggtgctcaatt---attttcttttatttaagctgcaagagaatctaaatatcctttgaatgcatttctgctgactagGATAAAGCCACCGTAGAAATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATCATTCATGGAGATGGTTCCAAAGAGATCAAAGTTGGTGAG

upper sequence: GRMZM2G015132_T01 (Zea mays), 3'ss of exon 6
lower sequence: AT1G54220.2 (Arabidopsis thaliana), 3'ss of exon 7
gtaagtttcttaatttgtagaagttatggaacatatgttatttgtatttatttttacttaagctgtagcagaacgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG
                |||      ||| |   |  ||    | |   |||||||| ||    |||    |    |||||||| |     |   || || | |  |||||||||| ||||| || ||||| |||||||||||||||||||| || |||||| |  | |  || ||| | || || ||| |||| || |||
----------------gtaatgtcaatgtagattgagtctcatttccgtatttttattttca-tgttttgg----aaataactct--gactaggttatgttcagtagGACAAAGCAACTGTCGAAATGGAATGCATGGAAGAGGGCTATCTCGCCAAGATTGTAAAGGCGGAAGGTTCAAAAGAAATTCAAGTCGGCGAG

upper sequence: GRMZM2G015132_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv11s0016g01870.t01 (Vitis vinifera), 3'ss of exon 7
gtaagtttctta-atttgtagaagttatggaacatatgttatttgtatttatttttacttaagctgtagcagaacgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG
||| | | |||  |  |||     || |  ||  ||  |  ||  |   |  |     || |  |  ||   | |     || |||| | |   ||| |       |||| ||||| ||||| |||||||| |||||||| || || ||||||||||||||  | | ||||||||| || || ||||| ||||||||  ||
gtagggtccttgtagctgtcccctttttcaaatttac-tagttgatcaatgatccaggtttatgtaaagtc-atcctgtcaaatttttaatcattttat-------agGATAAAGCAACTGTTGAGATGGAATGCATGGAGGAAGGATATCTTGCTAAGATAGTACTTGGAGATGGGGCAAAAGAGATCAAAGTTGGGCAG

upper sequence: GRMZM2G015132_T01 (Zea mays), 3'ss of exon 6
lower sequence: Vv09s0002g01800.t01 (Vitis vinifera), 3'ss of exon 8
--------------------gtaagtttcttaatttgtagaagttatggaacatatgttatttgtatttatttttacttaagctgtagcagaacgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG
                    ||  |||  |||| || |   | |||  | |   |      ||   |     |||||| |   | | | | |  ||  |     ||   |||| ||    |    |||| ||||| || || |||||||| ||||||||||| || || ||||||||||| ||||| |||||||| || || || |||||||||||||||
gtagggcccttgtactctgagtctgttggttaaattctctcaattaatgga-gcactccggttaggt---gctttactca---tctggaaaactgacgtttacctttcattgaattgatatttgtagGATAAAGCAACAGTTGAGATGGAATGCATGGAAGAAGGATACCTTGCTAAGATAATTCAAGGAGATGGGGCAAAAGAAATTAAAGTTGGTGAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|74241792|gb|DT649706.1|DT649706
EST:     AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACT                         GACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
genomic: AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
EST: gi|76932164|gb|DV172957.1|DV172957
EST:     AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACT                         GACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
genomic: AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
EST: gi|71441954|gb|DR823004.1|DR823004
EST:     AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACT                         GACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
genomic: AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
EST: gi|211501920|gb|FL162229.1|FL162229
EST:     AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACT                         GACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG
genomic: AGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 147

GGAAACATTGCCAAGTGGCTTAAGAAGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 208

GGAAACATTGCCAAGTGGCTTAAGAAGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 221

GGAAACATTGCCAAGTGGCTTAAGAAGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 224

GGAAACATTGCCAAGTGGCTTAAGAAGGAAGGAGACAAGGTCTCCCCTGGTGAGGTTCTCTGCGAAGTGGAAACTgtaagtttct ... tggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttacttaagctgtagcagaacgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG
                                         ttttt  CT-rich tract
                       aaataactttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtttcttaatttgtagaagttatggaacatatgttatttgtatttatttttacttaagctgtagcagaacgaaataacttttgagttgatttttggtgatcagGACAAAGCCACTGTGGAGATGGAGTGCATGGAAGAGGGCTATCTTGCTAAGATTATTCATGGAGATGGTGCGAAGGAGATTAAAGTTGGTGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CATGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAT