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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttttggcggcgccagtattttcgattttcctcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA

Basic information

species Physcomitrella patens
transcript PP1S398_18V6.1
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: LOC_Os01g12730.2 (Oryza sativa), 3'ss of exon 2
ttttggcggcgccagtattttcgattttcct--cggtatctc-taactcgtgaaaccgttgcctcatctcgactaacatacgta---gagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
           | || || |  |    ||       || ||  | | | || |||| ||       |||  | | ||     ||   | | ||     |     |||||||||||||||| ||||| ||||| |||||||| || ||||| || |||||||| || || || || ||||| ||||| ||||| || |||||||  |
------aaatactagaatgtatggcaatcaaagtaataccttgtcatttgtcaaacagtaattctgtctacatttacgctaatacttgtgtttacctggaacttagATTTGGGACACAGCGGGACAGGAGCGATTTCAGAGTCTTGGTGTGGCATTTTACCGGGGAGCTGACTGCTGTGTTCTTGTATATGATGTCAATGTTACCA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM2G158887_T02 (Zea mays), 3'ss of exon 2
--ttttggcggcgccagtattttcgattttcctcggtatctcta-actcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
    |           |||  |||    |     | | |    | |   || |||  ||    ||  || |  ||   ||  ||      | |||  |   |||| |||||||| ||||| || ||  |||||||||| ||||| ||||| || ||||| || ||||||||||||||||| |||||||| ||||||  |||||
tgcgtacataataaatgtagattcagaataatatgatgtgaagagaaatgtcaaag-gtattgtccgctggtttagggtatttaacaacatatct--ttgtagATATGGGACACTGCTGGCCAGGAAAGGTTTCAGAGTCTCGGCGTTGCATTCTACCGGGGAGCAGATTGTTGTGTCCTAGTCTATGATGTTAATTCTATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM5G811797_T05 (Zea mays), 3'ss of exon 3
-----ttttggcggcgccagtattttcgattttcctcggtatctct--aactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
       |  |        |||  | |   ||       | ||     || |    ||    ||| ||  ||| |  ||   |   ||     | ||        |||| |||||||| ||||| || ||  |||||||||| || || ||||| || ||||| || ||||||||||||||||| |||||||| ||||||  |||||
gtgcacataagaaatctatgtagatccagattaatatcatgtcaagagaaatgtcaaaggtattgtct--tctggtttatggtgtttaacaagtcttt-----gtagATATGGGACACTGCTGGTCAGGAAAGGTTTCAGAGTCTTGGCGTTGCATTCTACCGGGGAGCAGATTGTTGTGTCCTAGTCTATGATGTTAATTCTATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM5G811797_T04 (Zea mays), 3'ss of exon 3
ttttggcggcgccagtattttcgattttcctcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA---------------------------------
                           |     ||      || |    ||    ||| ||  ||| |  ||   |   ||     | ||        |||| |||||||| ||||| || ||  |||||||||| || || ||||| || ||||| || ||||||||||||||||| |||||||| ||||||  |||||                                 
--------------------------atatcatgtcaagagaaatgtcaaaggtattgtct--tctggtttatggtgtttaacaagtcttt-----gtagatatgggacactgctggtcaggaaaggtttcagAGTCTTGGCGTTGCATTCTACCGGGGAGCAGATTGTTGTGTCCTAGTCTATGATGTTAATTCTATGAAATCGTTTGATAATCTTCACAACTGGCGTGAAG

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GRMZM5G811797_T01 (Zea mays), 3'ss of exon 3
-----ttttggcggcgccagtattttcgattttcctcggt--atctctaactcgtgaaaccgttgc--ctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
     | |||    |   || | | |   |    |  | |  || ||          ||  ||      |  | ||  ||    || |  |         |  |   |||| |||||||| ||||| || ||  |||||||||| || || ||||| || ||||| || ||||||||||||||||| |||||||| ||||         
gtctctattgtgcacataagaaatctatgtagatccagattaatatcatgtcaagagaaatgtcaaaggtattgtcttctggtttatggtgtttaacaagTCTTTGTAGATATGGGACACTGCTGGTCAGGAAAGGTTTCAGAGTCTTGGCGTTGCATTCTACCGGGGAGCAGATTGTTGTGTCCTAGTCTATGATGTTA---------

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA07G00660.1 (Glycine max), 3'ss of exon 3
--ttttggcggcgccagtattttcgattttcctcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
  |  |   |         || |  ||| |        | |  |  |  || ||  |  |  | |||      ||   |  | |  ||     | |   ||||||||||| || ||||| ||||||||||||||||| || || || || || ||||||||||||||||| ||||||||||| ||||| || || || ||||
tgtcatattgattaagcagttctgtattctgtgatccgtttgaagtttatggaagaga-gattaatcatttaaaattgagat-gttctcaccatacatgcagATTTGGGATACTGCTGGTCAAGAGCGGTTTCAGAGTCTTGGCGTGGCTTTCTACCGTGGTGCAGATTGCTGTGTCCTTGTTTATGATGTAAACGTCATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA08G21940.1 (Glycine max), 3'ss of exon 3
ttttggcggcgccagtattttcgattttcctcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
| |      |   |     |||  | ||| | | | | | | ||   |   |  |     | |  |     ||  |  |  |  |||    | |   ||||||||||| || ||||| ||||| |||||||| || || ||||| || || ||||||||||||||||| ||||||||||| ||||| || || || ||||
tgtcatattcattaagcagttctgtattctttgatctgtttgacgtttatgaaagagagattaattatctaaaattgagatgttcttaccatacatgcagATTTGGGATACTGCTGGTCAAGAACGGTTTCAAAGTCTTGGTGTGGCTTTCTACCGTGGTGCAGATTGCTGTGTCCTTGTTTATGATGTAAACGTCATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA12G04830.1 (Glycine max), 3'ss of exon 3
-ttttggcggcgccagtattttcgattttcctcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
 | |   |  |  |     |||| || |   |    |  | ||  |    | |   |    |  | |   || |   |  |  |  || ||    | |||||||||||| |||||||| |||||  | || || ||||| || ||||| || || |||||||| ||||||||||| ||||| ||||| ||||||   ||||
gtctattctacatctcatatttctatgtctttttaaagttttaggttacaatagt-tcaaattttattttcttattaattttaatgttcttaactttacagATTTGGGATACAGCTGGCCAAGAAAGATTCCAAAGCCTTGGAGTTGCTTTCTATCGTGGTGCTGATTGTTGTGTTCTTGTATATGATGTTAATTCAATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: GLYMA11G12630.3 (Glycine max), 3'ss of exon 3
ttttggcggcgccagtattttcgattttcctcg-gtatctctaactcgtga-aaccgttgcctcatctcg--actaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
 | |       |   |||||    || || |     |  | ||| |    | |  ||     | || | |  | |||  | | |     || ||    | |||||||||||| |||||||| |||||  | || || ||||| || ||||| || || |||||||| ||||||||||| ||||| ||||| ||||||   ||||
ctattctacatctcatatttctatttgtctttttaaagttttaagttacaatagtcgaaattttattttgttattaatttccatg----ttcttaactttacagATTTGGGATACAGCTGGCCAAGAAAGATTCCAAAGCCTTGGAGTTGCTTTCTATCGTGGTGCTGATTGTTGTGTTCTTGTATATGATGTTAATTCGATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: AT3G18820.1 (Arabidopsis thaliana), 3'ss of exon 2
--------------------------------------------------------ttttggcggcgccagtattttcgattttcctcggt-atctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
                                                        || ||       ||   |||   ||||   |   | | || |    | |||   |  |  ||  |  |        ||| | |    | |  |  | |||||| ||||| ||||||||||| ||  ||||||||||||| ||||| ||||||||||||||||| ||||| ||||| ||||| ||||| || |||   ||||
gttcctactagtgctttattagttagtcactcaatattttttttgaagtaagatgcttgtgctcagctcaacctttcacatttgtgtgaattacctttcttgcttgagctcactcacttgttcctgtc--tttactttgtcaatgtgtttattacagATCTGGGATACAGCTGGACAGGAAAGGTTTCAGAGCCTTGGTGTAGCCTTTTACCGTGGTGCTGATTGCTGTGTTCTTGTATATGATGTCAATTCCATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: AT1G49300.1 (Arabidopsis thaliana), 3'ss of exon 3
ttttggcggcgccagtattttcgattttcc-tcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctt---tttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
    | |      ||| | || ||||     | | |  |    |||    | |  | |  ||   || ||| |  | |  ||  |  |   ||| |  | |||||| |||||||||||||| |||||  |||||||||| || |||||||| ||||| |||||||| ||||| ||||| ||||| ||||| || ||       |
----gaccataagagtgtcttagattaagaattgatcccaagtactatatatattgctctctttgcttgaccataagagctaaggtcttgctttttttttacagATCTGGGACACAGCTGGTCAAGAAAGGTTTCAGAGTCTTGGTGTTGCGTTTTATCGTGGTGCTGATTGCTGTGTTCTTGTATATGATGTCAACTCCGCAA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: Vv09s0002g04220.t01 (Vitis vinifera), 3'ss of exon 3
ttttggcggcgccagtattttcgattttcctcggtat--ctctaactcgtg-aaaccgttgcctcatctcgactaacatacgtagagctttttctgctga--cagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
    |||   |  ||  | ||  |   |    ||| |  || || ||   | |||   ||   | |     | | |   ||      || ||| |    |  ||||| ||||||||||||||||||||  |||| || ||||| ||||| || ||||| ||||||||||| || ||||| ||||| ||||| || ||||| ||||
----ggcataga-agcttgttgaaaactgaatggtttttctttagctgacggaaatgatttgtttacaattatttaaggacaacattctgtttttatatattcagATATGGGACACAGCTGGACAAGAAAGGTTCCAAAGCCTTGGTGTGGCTTTTTATCGTGGTGCAGACTGCTGTGTTCTTGTGTATGATGTGAATGTGATGA

upper sequence: PP1S398_18V6.1 (Physcomitrella patens), 3'ss of exon 2
lower sequence: Vv03s0063g02420.t01 (Vitis vinifera), 3'ss of exon 4
ttttggcggcgccagtattttcg-attttcctcggtatctctaactcgtgaaaccgttgcctcatctcg-actaacatacg---tagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
     |   |   |   | |  | || |   || |  | | ||  | |  ||   | |  | | | | |  |||| ||       |   | |||  |  || ||||| ||||| |||||||| || ||  | || || || || |||||||| || ||||||||||| ||||| ||||| ||||| ||||| |||||||| ||||
-----gtaccattaaattctgggtatgtctttctgagtttttacttgggtaacatgctaacactttttgcgctaaaatctagtccacttcctttcgttttggcagATCTGGGATACAGCTGGCCAGGAAAGATTCCAAAGTCTAGGTGTTGCTTTCTACCGTGGTGCTGATTGCTGTGTTCTTGTATATGATGTTAATGTGATGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|162275204|gb|FC451180.1|FC451180
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|162239905|gb|FC415744.1|FC415744
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|22491664|gb|BU051587.1|BU051587
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|162278758|gb|FC454868.1|FC454868
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|162239904|gb|FC415743.1|FC415743
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|100394822|gb|BY989942.1|BY989942
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|208390030|gb|DC938085.1|DC938085
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|208365879|gb|DC951136.1|DC951136
EST:     ATTTTTGGNCAAAAGNAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTTCAGAGCCTCGGTGTTGCCTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGG-TTTCAGAGCCTCGGTGTTGCCTT
EST: gi|18342622|gb|BJ174657.1|BJ174657
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
EST: gi|100391677|gb|BY989497.1|BY989497
EST:     ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAG                         ATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT
genomic: ATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 29 (upstream exon), 33 (downstream exon)
Score: 9

GTATGTTAACAAGAAGTTCAGCAACCAGTATAAGGCCACGATTGGAGCAGATTTTTTGACAAAAGAAGTGCAAGTCGAAGACAGGCTTGTCACAATGCAGgtcagtaatc ... ctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA
                      gactaac  putative branch site (score: 9)
                                       ctttttctgct  putative PPT
                         taacata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttttggcggcgccagtattttcgattttcctcggtatctctaactcgtgaaaccgttgcctcatctcgactaacatacgtagagctttttctgctgacagATTTGGGACACAGCTGGACAAGAGCGGTTTCAGAGCCTCGGTGTTGCCTTTTACCGTGGTGCAGATTGTTGTGTCCTTGTCTATGACGTTAATGTTATGA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGAT