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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtaaacagtttagcatttttttcaagtcactttgacaatttgagcagggccatatatgacctctctcttctgttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAG

Basic information

species Oryza sativa
transcript LOC_Os01g50770.2
intron # 6
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os01g50770.2 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G162145_T01 (Zea mays), 3'ss of exon 6
gtaagtaaacagtttagcatttttttcaagtcactttgacaatttgagcagggccatatatgacctctctcttctgttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAG
||||| ||| | | |  |  |||| |  |   | |  |     |    |     ||   ||||||  ||||     || ||  |||||| ||  | || |||||||| |||||||||||||||||||| |||||||||||||| |||||||| ||||||||||||||||| |||||||| |||
gtaagcaaaaaatatttctcttttcttcaccaaatccgttgcatc--actaattca-gcatgaccattctccc---ttctttttagGTGTGTACGTTTAGCTAGATTCGAGAATGATAGAACTATATCGTTCATACCTCCTGATGGATCTTTTGATCTAATGACATACAGGCTTAGCACTCAG

upper sequence: LOC_Os01g50770.2 (Oryza sativa), 3'ss of exon 6
lower sequence: GRMZM2G436835_T02 (Zea mays), 3'ss of exon 7
gtaagtaaacagtttagcatttttttcaagtcactttgacaatttgagcagggccata-tatgacctctctcttctgttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAG
    |||| ||   ||    | ||||   | ||||    |  ||  | ||       | | |||||  ||||     || ||  |||||| ||  | |||||||||||||||||||||||||||||||| |||||||||||||| |||||||| ||||||||||||||||| |||||||| |||
----gtaagcaaaatactgctcttttt--ggcactaaatctgttgcatcactaattcagtgtgaccattctccc---ttctttttagGTGTGTACGTTTGGCTAGATTTGAGAATGATAGAACTATATCGTTCATACCTCCTGATGGATCTTTTGATCTAATGACATACAGGCTTAGCACTCAG

upper sequence: LOC_Os01g50770.2 (Oryza sativa), 3'ss of exon 6
lower sequence: Vv00s0225g00220.t01 (Vitis vinifera), 3'ss of exon 6
----------------------------------------------------------------------------gtaagtaaacagtttagcatttttttcaagtcactttgacaatttga--gcagggccatatatgacctctctcttctgttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAG
                                                                             |  | |||      ||   |  |||   |  |  ||   | | | |  ||| |  |  | |  |  | |   |  | || ||  ||| || ||  | |||||| |||||||||||||| |||| |||||||| || ||||| || |||||||| ||||| |||||||| || || || || |||
gtatgtagtcaaacttttttgtgtagttgtaatctgctgctggtaattaatttttattttttttctgataattatcatgtgcaaaactgcaagggctgatttattgcaaactttctattcttactgcaagatctcaaactaaatgtgcttatttttgtt-ttagATGTGTTCGTTTGGCTCGATTTGAGAATGATCGAACAATATCCTTTATTCCTCCAGATGGATCTTTTGATCTCATGACATATAGGCTCAGTACTCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|27554506|gb|CA998406.1|CA998406
EST:     CGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCA                         GTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACC
genomic: CGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAgtaagtaaac ... ttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACC
EST: gi|163923569|gb|EG713310.1|EG713310
EST:     CGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCA                         GTGCGTGAGGTTGGCTAGATTTGAGGATGATAGAACTATATCCTTCATACC
genomic: CGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAgtaagtaaac ... ttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 71 (upstream exon), 69 (downstream exon)
Score: 45

CTGAGTGCAAACTTGGATTGAACGATAGGGTTCTTTTGGAGGCTCAAGGCCGAGCTACTAAAGGAAAAGCAATAGATCTTGATGATATTAAATTTCACCAgtaagtaaac ... ttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cactttgacaatttgagcagggccatatatgacctctctcttctgttattactagGTGCGTGAGGTTGGCTAGATTTGAGAATGATAGAACTATATCCTTCATACCTCCTGACGGATCTTTCGATCTAATGACATACAGACTTAGCACCCAG
  ctttgac  putative branch site (score: 45)
                                cctctctcttctgtta  putative PPT
                                             ttattacta  TA-rich tract