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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...attcccttttggttatttaatcaacatcacaagttgcaacaatttcttcttaaaatgccctgttcagctcagctgatatttatttttgttcaccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG

Basic information

species Zea mays
transcript GRMZM2G011068_T01
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G011068_T01 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os05g44590.2 (Oryza sativa), 3'ss of exon 5
-----attcccttttggttatttaatcaacatcacaagttgcaacaatttcttcttaaaatgccctgttcagctcagctgatatttatttttgttcaccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG
||| | | ||| || | | |||| | ||| | | || ||| || | ||||| ||||| | || || | | ||||| | ||| |||| ||| |||| |||||||||||||||||||| |||||||||||||||||||||||| | | | | |||||||| ||||||||| |||
cggtcattagccccttagtat--gatttatgtataaagtgatgtataattcctttcgaacagcctcaca-aggtaagctggtattt--tgttatttctttaacagATAAGACAACTTGGGAGGATGGATGATGGTTCATTAAATGTTGTAGCCCGTGGGCAGCAAAGATTTCGTCTGAAGCGTCATTGGATGGATGTTGATGGGG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71766061|gb|DR963998.1|DR963998
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
EST: gi|71448594|gb|DR829644.1|DR829644
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
EST: gi|71436330|gb|DR817380.1|DR817380
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
EST: gi|31664610|gb|CD573543.1|CD573543
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTTG
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTG
EST: gi|16926249|gb|BM079317.1|BM079317
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
EST: gi|211143110|gb|FL166797.1|FL166797
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTT
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTT
EST: gi|76288774|gb|DV028342.1|DV028342
EST:     TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAG                         ATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT
genomic: TCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 110

GTTCTCATGCGACGACTCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 58

GTTCTCATGCGACGACTCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 47

GTTCTCATGCGACGACTCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG


Block sizes: 45 (upstream exon), 48 (downstream exon)
Score: 42

GTTCTCATGCGACGACTCCCAAATCATCGACATTATGCTACTGCTTCAGTTGGAACAACCGCAGAGgtcttgcaca ... caccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cttcttaaaatgccctgttcagctcagctgatatttatttttgttcaccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG
                         agctgat  putative branch site (score: 42)
 tttttgttcacctt  putative PPT
 tgatatttatttttgt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
attcccttttggttatttaatcaacatcacaagttgcaacaatttcttcttaaaatgccctgttcagctcagctgatatttatttttgttcaccttatagATACGTCAATTTGGAAGGTTGGAAGATGGTTCATTAAATGTTGTTGCCCGTGGGCAGCAAAGATTTCGTTTAAGGAGGCATTGGATTGATGTTGATCGGG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGGAG
- - - ttttggt
- - - - - - - - - - - aacatca
- - - - - - - - - - - - - - - -agttgca
- - - - - - - - - - - - - - - - - - - - - - - - - - - -tgccctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tcagctg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttttgtt