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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tatatgacaaaattactattattaaaatggaattcaattcctatgcgtaagtaaattgagttggagaagaattctgaactttatatggaattttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT

Basic information

species Zea mays
transcript GRMZM5G885392_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G885392_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os08g41460.8 (Oryza sativa), 3'ss of exon 3
tatatgacaaaattactattattaaaatggaattcaattcctatgcgtaagtaaattgagttggagaaga-attctgaactt---tatatggaattttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT
  | |    | || ||  ||||| | ||       | ||    |||  | || |      || |  ||   || ||| | |     || ||  |||||  |||||||||||||||||||||||| || ||||| |||||||| || ||||||||||| ||||| ||| |||||||| ||||
--tgtagagacatgacc-ttattcacatcatcactattttgggtgctaatgtgatgctgtttagttaatctatcctggagtaacaaatttgttatttt-cgcagGTCTACAGTGACTTGACTGTCGATTACTTCAAGATGTTCCTGCTAAATGTTCCAGCAGCAGTTTTAGCTCTGACACT
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32924858|gb|CF029670.1|CF029670
EST:     ACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: ACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|71760700|gb|DR958637.1|DR958637
EST:     GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|32925008|gb|CF029820.1|CF029820
EST:     ACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: ACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|32926006|gb|CF030818.1|CF030818
EST:     ACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: ACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|6827379|gb|AW331022.1|AW331022
EST:     GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|32926318|gb|CF031130.1|CF031130
EST:     GTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: GTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|78074964|gb|DV503398.1|DV503398
EST:     GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|13381842|gb|BG458517.1|BG458517
EST:     GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGTATATTATGTTGCTCCTAAAT
genomic: GAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTG-A-A-GATGTTGCTCCTAAAT
EST: gi|32942294|gb|CF047113.1|CF047113
EST:     GTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: GTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
EST: gi|110538710|gb|EE042664.1|EE042664
EST:     ATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAG                         GTTTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT
genomic: ATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 40 (upstream exon), 44 (downstream exon)
Score: 14

AAAGAAGGAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 92

AAAGAAGGAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT


Block sizes: 21 (upstream exon), 48 (downstream exon)
Score: 3

AAAGAAGGAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 81

AAAGAAGGAAGCGACATTGTACAGGAAGGTCCAAGATGCAGCCTACGGAGTCGAAAGgtatgtactg ... ttttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

cgtaagtaaattgagttggagaagaattctgaactttatatggaattttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT
                                  tttatatggaatttt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tatatgacaaaattactattattaaaatggaattcaattcctatgcgtaagtaaattgagttggagaagaattctgaactttatatggaattttgtgcagGTCTACAGTGACTTGACTGTTGACTACTTGAAGATGTTGCTCCTAAATGTTCCCGCAGCTGTTGTAGCTCTGGCACT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGTT
- - -gacaaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -gagaagaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -ttctgaa