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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagcattccatcttcctaacctttaatttcccctttttgatattttttagttaataatgatatatggttgtggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG

Basic information

species Zea mays
transcript GRMZM2G131118_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G131118_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os11g06930.1 (Oryza sativa), 3'ss of exon 6
gtaagcattccatc----------------ttcctaacctttaatttcccctttttgatatttttta-gttaataatga-tatatggttgtggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG
||||||||| ||                  |   ||  ||  | |||    | ||| |||||| ||| |  | |||||  ||| |  ||||| | || ||||||||||||||||| ||||| |||||| | |||||| | |||||||||||||||| ||||||||||
gtaagcatttcaatacttttatggaataattctttattctagagtttatattattttatatttcttaaggcactaatggctatgtcattgtgaaattcagCTCAAAGGATGTGCTTATGCGCGTTGTGACACAAAATCTCTCTCCTGAGTTAACTCATGTAGAAAAG

upper sequence: GRMZM2G131118_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA05G32930.1 (Glycine max), 3'ss of exon 6
gtaagcattccatct-tcctaacctttaatt-----tcccctttttgatattttttag-ttaataatgatat-atggttgtggatttt-----------agCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG
 | |   | |||||   || || | |   ||     |||| |  ||  || |||  |  ||||| ||  ||  ||| ||   | |||            || ||||||||  | || |||||||||||||| |||||| ||||||||||||| ||||| || ||||||
-tgattttcccatccgccccaatcctctgttactcatcccatccttcctaatttgcaccttaatcattgtaagatgcttaatgttttctatactcatgcagTTCAAAGGACATACTAATGCGTGTTGTGGCTCAAAATCTTTCTCCTGAGTTGACTCTCGTGGAAAAG

upper sequence: GRMZM2G131118_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA08G00560.2 (Glycine max), 3'ss of exon 6
------------------------------------------------------------------------------------gtaagcattccatcttcctaacctttaatttcccctttttgatattttttag-ttaataatgatat-atggttgtggatttt-----------agCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG
                                                                                    | |    |  ||   |        | || |     | | || ||      || ||||| ||  ||  ||| ||   | |||            || ||||||||  | || |||||||||||||| |||||| ||||||||||||| ||||| || ||||||
gtaagatctttttcagaggctttaatctcttcttttggaagttgagggtgtggaataatatacaaaattgcatgttttcgtagtgaagaactctcaaggtttggggtataaaatattattctctggtaggagccaggttaatcattgtaagatgcttaatgctttctatgctcatgcagTTCAAAGGACATACTAATGCGTGTTGTGGCTCAAAATCTTTCTCCTGAGTTGACTCTCGTGGAAAAG

upper sequence: GRMZM2G131118_T01 (Zea mays), 3'ss of exon 4
lower sequence: Vv07s0031g02750.t01 (Vitis vinifera), 3'ss of exon 6
------------------------------------------------------------------gtaagcattc-catcttcctaacctttaatttccccttt-ttgatattttttagttaataatgatatatggttgt-------ggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG
                                                                   |  |||||  || | |   |     |  |||    | | || |||| |  | ||   || | || | || ||||          ||  || ||||||||| | || ||||| |||||||| |||||  ||||||||||||| ||||| || ||||||
gtattattcatttctctactggctcctatcttttctgactgtaactttgtatcaacaaggttttatttttgcattagcagcatttcaggaggtgttttgatgtctgtttatatctactggtggttacttatgtctgtttgtttcaactaacttgcagTTCAAAGGATATTCTTATGCGGGTTGTGGCACAAAACCTTTCTCCTGAGTTGACTCTGGTGGAAAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 31 (downstream exon)
Score: 28

CCCTTTCAGATCCTGTTTGTGTAGCAACCCGGAAAATGCGCGACTTACGTGTTAATTCAGTAATTATTATGGCTGGAAATTCGCTTCATGGGATCTTTACgtaagcattc ... tggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG


Block sizes: 47 (upstream exon), 27 (downstream exon)
Score: 22

CCCTTTCAGATCCTGTTTGTGTAGCAACCCGGAAAATGCGCGACTTACGTGTTAATTCAGTAATTATTATGGCTGGAAATTCGCTTCATGGGATCTTTACgtaagcattc ... tggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG


Block sizes: 46 (upstream exon), 31 (downstream exon)
Score: 12

CCCTTTCAGATCCTGTTTGTGTAGCAACCCGGAAAATGCGCGACTTACGTGTTAATTCAGTAATTATTATGGCTGGAAATTCGCTTCATGGGATCTTTACgtaagcattc ... tggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG


Block sizes: 49 (upstream exon), 31 (downstream exon)
Score: 19

CCCTTTCAGATCCTGTTTGTGTAGCAACCCGGAAAATGCGCGACTTACGTGTTAATTCAGTAATTATTATGGCTGGAAATTCGCTTCATGGGATCTTTACgtaagcattc ... tggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

atttcccctttttgatattttttagttaataatgatatatggttgtggattttagCTCAAAGGATGTGCTGATGCGTGTTGTGGCCCAAAATATTTCTCCTGAGTTAACTCTTGTAGAAAAG
                       agttaat  putative branch site (score: 19)
        tttttgatatttttta  TA-rich tract