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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtacgttaacgacactgcctgccgtgggccggaatttgctagttcttgaacgaacattgtccagttataggctataacataataaatatatacaatgtgcttcgaaacttttttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAGGAGCATTGTATGACAAATGCAGGGGAGAGATTGCCAAGGCGATGAATCT

Basic information

species Zea mays
transcript GRMZM2G085711_T02
intron # 6
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76011900|gb|DT939070.1|DT939070
EST:     GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGT-                         GTAAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAA
genomic: GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGT-AAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAA
EST: gi|67027734|gb|CO456483.1|CO456483
EST:     GATGTCCAAGAACGGACCATTCAGCTCCTGCGTGCTGCGCGGATTTAGTG                         GTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAG
genomic: GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAG
EST: gi|86474415|gb|DY240785.1|DY240785
EST:     GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTG                         GTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAG
genomic: GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAG
EST: gi|211540961|gb|FL453890.1|FL453890
EST:     GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTG                         GTAAATATACCTACAATGGACAGCAGTTCG
genomic: GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCG
EST: gi|93283843|gb|EB676107.1|EB676107
EST:     GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTG                         GTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAG
genomic: GATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 3 RNA-Seq libraries.


Block sizes: 40 (upstream exon), 14 (downstream exon)
Score: 2

CTACTTGCGCTATGGTTCCTTTGCATCACGAGCGGAGATTCTGATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAGGAGCATTGTATGACAAATGCAGGGGAGAGATTGCCAAGGCGATGAATCT


Block sizes: 36 (upstream exon), 45 (downstream exon)
Score: 6

CTACTTGCGCTATGGTTCCTTTGCATCACGAGCGGAGATTCTGATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAGGAGCATTGTATGACAAATGCAGGGGAGAGATTGCCAAGGCGATGAATCT


Block sizes: 36 (upstream exon), 48 (downstream exon)
Score: 7

CTACTTGCGCTATGGTTCCTTTGCATCACGAGCGGAGATTCTGATGTCCAAGAACGGACCATTCAGCTCCTGCATGCTGCGCGGATTTAGTGgtacgttaac ... ttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAGGAGCATTGTATGACAAATGCAGGGGAGAGATTGCCAAGGCGATGAATCT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

taggctataacataataaatatatacaatgtgcttcgaaacttttttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAGGAGCATTGTATGACAAATGCAGGGGAGAGATTGCCAAGGCGATGAATCT
    ctataac  putative branch site (score: 7)
                                        cttttttttgt  putative PPT
     tataacataataaata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtacgttaacgacactgcctgccgtgggccggaatttgctagttcttgaacgaacattgtccagttataggctataacataataaatatatacaatgtgcttcgaaacttttttttgtgaagGTAAATATACCTACAATGGACAGCAGTTCGATGCGACTGCTAGACCAGAAGGAGCATTGTATGACAAATGCAGGGGAGAGATTGCCAAGGCGATGAATCT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGCA