ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtaaacattgtgaagtaacctacagtttattttatgtgtgcatcatgtataaacttatcacataatcaccattttcatatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG

Basic information

species	Oryza sativa
transcript	LOC_Os09g29404.1
intron #	18
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os09g29404.1 (Oryza sativa), 3'ss of exon 18
lower sequence: GRMZM2G076508_T01 (Zea mays), 3'ss of exon 6

gtaaacattgtgaagtaacctacagtt-tattttatgtgtgcatcatgtataaacttatcacataatcaccattttcatatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG
       ||||    |   | | ||||   ||||| ||||  |    ||||||  | || |  ||  || |||    ||| |  ||||||| ||||||||| ||||||| |||||||||||||| || |||||||| |||||||||||||||||||||||||||  ||| |||||||| || ||||
-----gtttgtattatcgaccatagttaccttttacgtgta-actgagtataacttgatgaatta--catcat----atacaatagGGCACTCCAATGATGCTGATGGGAGATGAATATGGTCACACGCGTTATGGAAACAACAATAGCTATGGACATGATACTCACATAAATAATTTTCAGTGGG

upper sequence: LOC_Os09g29404.1 (Oryza sativa), 3'ss of exon 18
lower sequence: GRMZM2G150796_T01 (Zea mays), 3'ss of exon 19

gtaaacattgtgaagtaacctacagtt-tattttatgtgtgcatcatgtataaacttatcacataatcaccattttcatatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG
||  | ||| |  |     | | ||||   ||||| ||||  |    |||||| ||||| |  ||     |||| | ||| |  |||| || ||||||||| ||||||| |||||||||||||| ||||||||||| |||||||||||||||||||||||||||  ||| |||||||| || ||||
gtttatattatcga-----ccatagttaccttttacgtgta-actgagtataa-cttatgagtta-----cattat-atacaatagGGTACTCCAATGATGCTGATGGGAGATGAATATGGTCACACACGTTATGGAAACAACAATAGCTATGGACATGATACTCACATAAATAATTTTCAGTGGG

upper sequence: LOC_Os09g29404.1 (Oryza sativa), 3'ss of exon 18
lower sequence: GLYMA06G10540.1 (Glycine max), 3'ss of exon 16

------gtaaacattgtgaagtaacctacagtttattttatgtgtgcatcatgtataaacttatcacataatcaccat---tttcatat------atcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG
      | |||| |   | |  ||  ||   |||   || | | ||    |||     ||| |   |||||| |   |   |||  ||       | ||||| || ||||||||| | ||||| ||||||||||| ||||| ||  |||| || |||||||| ||||| |||||||||  ||||||||||||        
tttaaagaaaacttgaagcacaaatatatgattttcattttttttgtgctatgctatcactgaaaccataatgattttaaattttgtaatgaatgaccagGGTACACCAATGATGCTAATGGGGGATGAATATGGCCATACTCGCAATGGAAATAACAATAGTTATGGGCATGATACTGCCATTAATAATTTTTT-----

upper sequence: LOC_Os09g29404.1 (Oryza sativa), 3'ss of exon 18
lower sequence: AT4G09020.1 (Arabidopsis thaliana), 3'ss of exon 19

gtaaacattgtgaagtaacctacagtttattttatg-tgtgcatcatgtataaacttatcacataatcaccattttcatatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG
||    |||       ||| |||| |   ||  ||  |||  |  || | ||||   ||   | |   |   |  |||   ||||||| || ||||||||| | ||||| || |||||||| |||||||| ||||| |||||||||||||| ||||||||||| | | |||| |||||||| ||| 
gtgcgtattt---gccaacatacactcgtttaaatactgtctaaaat-tctaaatgcatatgaaaccgatggtgatcaatgatcagGGAACACCAATGATGCTAATGGGAGACGAATATGGACATACACGGTATGGTAACAACAATAGCTACGGACATGATACCTCCCTTAACAATTTCCAGTGGA

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|66947191|gb|AU312035.1|AU312035

EST:     TGCGTTCAAGACAAATGAAAAACTTCCATGTAGCTTTAATGATTTCTCAG                         GGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGG
genomic: TGCGTTCAAGACAAATGAAAAACTTCCATGTAGCTTTAATGATTTCTCAGgtaaacattg ... catatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGG

EST: gi|33797263|gb|CF324494.1|CF324494

EST:     TGCGTTCAAGACAAATGAAAAACTTCCATGTAGCTTTAATGATTTCTCAG                         GGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGG
genomic: TGCGTTCAAGACAAATGAAAAACTTCCATGTAGCTTTAATGATTTCTCAGgtaaacattg ... catatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 75 (upstream exon), 74 (downstream exon)
Score: 16

GAGAAACAAATGATCTGAATGTGTTAAGTCTGCGTTCAAGACAAATGAAAAACTTCCATGTAGCTTTAATGATTTCTCAGgtaaacattg ... catatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG

<

<

<

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

tttatgtgtgcatcatgtataaacttatcacataatcaccattttcatatatcagGGCACCCCAATGATGTTGATGGGCGATGAATATGGTCATACACGTTATGGGAACAACAATAGCTATGGACATGATACTTGCATTAATAATTTCCAATGGG