ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

...agcttttgttcaaaaaatgtattacagcaaaacagatatgtttctttttttaccaagtttttctcctgtaatgatgtgttaatctttttcattttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG

Basic information

species	Oryza sativa
transcript	LOC_Os01g52170.1
intron #	12
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: LOC_Os01g52170.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GRMZM2G129208_T01 (Zea mays), 3'ss of exon 11

------------------------------------------------------------agcttttgttcaaaaaat-gtattaca-gcaaaacagatatgtttctttttttaccaagtttttctcctgtaatgatgtgttaatctttttcattttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG
                                                            ||         || || | ||   |||  |      ||||    |   |  |    ||         || |  | ||   ||  | |||||| | ||| ||||||| ||||| || ||||| |||||||| |||| ||||||| |||||||||| ||||||||||| |||||||||||| |||||||||||||||| ||| |
gtaaatagactgaaagtagttcatggaagatgacctcatatacggacacaaaaaataataagaaagcaccaaataagttgtgcaacacacgttctggatacacatacataatacagaaaaagagacgcttttcttataaattgttatttttc-tgttgtcagTTTCTACAGCGGTGTCCTACGATAGATCCATTAAAGCAATCAGAGTGAAAGTGCCAGATTCAGACGAAGAGTTCTTGCTGCATCCAGCAACAGTTAGACG

upper sequence: LOC_Os01g52170.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GLYMA07G09820.1 (Glycine max), 3'ss of exon 12

---agcttttgttcaaaaaatgtattacagcaaaacagatatgtttctttttttaccaa--gtttttctcctgtaatgatgtgttaatct-ttttcattttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG
   ||   || |||     ||  |||  |     ||||||||   | | | |||| ||    | ||| | || | || ||    || | |  ||||| ||   |||||||||| || ||  ||||||||| |||||| |  ||||||  |||||| |||||||||||| | ||||| |||||  ||||||| |||||||| ||| |
ttcagaaattattctttctattgatttgaat---acagatatcagtttctctttagcattcatattttttctttgattataaaatactttactttcaatt---cagTTTCAACTGCGGTCACCTATGATAAATCAATCAAGGCAATCAAAGTGAAGGTACCAGATTCAAAGGAAGAATTCTTTCTGCATCCTGCAACAGTGAGACG

upper sequence: LOC_Os01g52170.1 (Oryza sativa), 3'ss of exon 12
lower sequence: GLYMA09G31980.1 (Glycine max), 3'ss of exon 12

---agcttttgttcaaaaaatgtattacagcaaaacagatatgtttctttttt-taccaagtttttctcctgtaatgatgtgttaatctttttcattttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG
   ||   || |||     ||  |||  |  | |  | ||  |||||| | |   |   |  |||| || |  | |||    | | |   ||||| ||   |||||||||| || ||  ||||||||| |||||| |  ||||||  |||||| |||||||||||| | ||||| |||||  ||||||| |||||||| ||| |
ttcagaaattattctttctattgattttaatacag-atatcagtttctctgtagcattcatattttttctttgattgaaaaataatttactttcaatt---cagTTTCAACTGCGGTCACCTATGATAAATCAATCAAGGCAATCAAAGTGAAGGTACCAGATTCAAAGGAAGAATTCTTTCTGCATCCTGCAACAGTGAGACG

upper sequence: LOC_Os01g52170.1 (Oryza sativa), 3'ss of exon 12
lower sequence: Vv11s0016g04250.t01 (Vitis vinifera), 3'ss of exon 11

agcttttgttcaaaaaatgtattacagcaaaacagatatgtttctttttttaccaagtt---tttctcctgtaatgatgtgttaatctttttcattttg-ccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG
     |    |    ||  ||||   |         |   |||     |   |   |||   |||   || || |         |   | ||| || || ||||| |||||||| ||  ||||||||| ||  || | ||||||| |||||||||||||||||||||| ||||||||| |  ||||||| || || || ||| |
----atcaaactgccaacatattttggggttgttactccatttggccctgagctttgttccatttggtctttattttaacaaatactatcttctttctgtccagTGTCAACAGCTGTCACCTATGATAAATTTATCAAAGCAATCAGAGTGAAAGTACCAGATTCAGAAGAAGAGTTCCTTCTGCATCCTGCTACTGTAAGACG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|29683979|gb|CB680254.1|CB680254

EST:     GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGG                         TTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG
genomic: GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG

EST: gi|27577753|gb|CB000448.1|CB000448

EST:     GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGG                         TTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG
genomic: GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG

EST: gi|66923650|gb|CX110498.1|CX110498

EST:     AAATCTTGGAGTTTGTGTTGTTCAGCAATGTGCTAAAATCAGACAACAGG                         TTTCAACAGCAGTTTTCTATGATAGATC
genomic: GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATC

EST: gi|38481133|gb|CF965222.1|CF965222

EST:     TGTGCTAAAATCAGACAACAGG                         TTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG
genomic: TGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG

EST: gi|29641473|gb|CB646480.1|CB646480

EST:     GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGG                         TTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG
genomic: GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG

EST: gi|29613739|gb|CB618752.1|CB618752

EST:     GAATCTTGGAGTTTGTGTTGTTCAGCAATGTGCTAAAATCAGACAACAGG                         TTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG
genomic: GAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 1 RNA-Seqlibrary.

Block sizes: 67 (upstream exon), 75 (downstream exon)
Score: 41

ATTCCTGAAGTAGTGGCTGATCCACAAGGGGATGTTGCTAAGACGTTTCAGAATCTTGGAGTTTGTGTTGTTCAGCAGTGTGCTAAAATCAGACAACAGGgtaagttagc ... attttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG

<

<

<

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

ttttttaccaagtttttctcctgtaatgatgtgttaatctttttcattttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG

                               tgttaat  putative branch site (score: 41)
                                     tctttttcattttgcc  CT-rich tract
                                 ttaatctttttcattt  TA-rich tract

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
agcttttgttcaaaaaatgtattacagcaaaacagatatgtttctttttttaccaagtttttctcctgtaatgatgtgttaatctttttcattttgccagTTTCAACAGCAGTTTCCTATGATAGATCAATTAGAGCAATCCGAGTGAAAGTACCAGATTCAGATGAAGAGTTCTTGTTGCATCCAGCAACAGTCAGAAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- cttttgt
- - - - - caaaaaa
- - - - - - - - - - - - cagcaaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - atgatgt