ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CCAGTTAGATCCTGATGATCTTAGTGAACAATTGAAGCGGCAAGGTGCTTCGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttcttacacttccatttaaatatacgcttttcttcaattctaatgcacatgtttcaactagttaccttattgctgtaaaatgtggaagttgtcca...

Basic information

species	Zea mays
transcript	GRMZM5G809546_T01
intron #	7
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM5G809546_T01 (Zea mays), 5'ss of exon 7
lower sequence: LOC_Os08g15460.2 (Oryza sativa), 5'ss of exon 7

CCAGTTAGATCCTGATGATCTTAGTGAACAATTGAAGCGGCAAGGTGCTTCGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttcttacacttccatttaaatatacgcttttcttcaattctaatgcacatgtttcaactagttaccttattgctgtaaaatgtggaagttgtcca-
 || || |||||||||||||||||||| ||||||||||||||||||||||| ||||| |||||  | ||||| |||||||||||||| |  || ||||||||||||| ||   ||||||||  |||| |   | |||||  |    | | |  || |||    ||  | | | ||   | |          |   |  || 
TCAATTGGATCCTGATGATCTTAGTGAGCAATTGAAGCGGCAAGGTGCTTCAATACCGCTTGTACGGCCAGGGAAAAGTACAGCTGCCTTCATAAAAACAgtgagttattgttcttccattagaataaatattgttctttcaccgca-tccgaatttttagtttatgtgcataatgtatatgttcctgctcatcagctcat

upper sequence: GRMZM5G809546_T01 (Zea mays), 5'ss of exon 7
lower sequence: GLYMA06G30020.1 (Glycine max), 5'ss of exon 7

CCAGTTAGATCCTGATGATCTTAGTGAACAATTGAAGCGGCAAGGTGCTTCGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttcttacacttccatttaaata---tacgcttttcttcaattctaatgcacatgtttcaactagttaccttattgctgtaaaatgtggaagttgtcca
 ||  | || ||||||||| | |||||||||||||| || ||||||||||| || |||||||| |||||||| | || ||||||| | | ||| |||||||| | | | | |  ||| | ||    |   | |  | ||||||   |   || |     ||||   ||   |  |  |  ||  |   | |  |         
ACAACTGGACCCTGATGATGTGAGTGAACAATTGAAACGTCAAGGTGCTTCAATTCCACTTGTCAGACCAGGCAGAACTACAGCTACATTTATCAAAACAgtaattccgtccttttctagttttgcactttgctatcttcttctgattatatactgtcttttctcttaaaaatatactccctccagtctttatataagaa---

upper sequence: GRMZM5G809546_T01 (Zea mays), 5'ss of exon 7
lower sequence: Vv11s0065g00940.t01 (Vitis vinifera), 5'ss of exon 7

CCAGTTAGATCCTGATGATCTTAGTGAACAATTGAAGCGGCAAGGTGCTTCGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttcttacacttccatttaaatatacgcttttc---ttcaattctaatgcacatgtttcaactagttaccttattgctgtaaaatgtggaagttgtcca
 || || || ||||||||| | |||||||| ||||| || |||||||| || || ||||||||  |||| || |||||||||||||| | ||||||||  || | ||  |    |   | | |||| |    | |     | || |  |  ||      ||| || |  | | |||  ||  ||||    || | || ||   
ACAATTGGACCCTGATGATGTGAGTGAACAGTTGAAACGTCAAGGTGCATCAATCCCACTTGTGCGACCCGGTAAAAGTACAGCTGCATTTATTAAAATGgtaattttctcttttcttaatgaaatgtgtagtctatcattgcattatttgtgtggtgtttttaatt--tcatcttgatgtggtaatgcatg-aggtggtttt

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|71315712|gb|DR794487.1|DR794487

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|60353250|gb|DN220223.1|DN220223

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|94473420|gb|EB702378.1|EB702378

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|149099085|gb|EE185105.2|EE185105

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|148963940|gb|EE015061.2|EE015061

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|148951011|gb|EC896663.2|EC896663

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|149098781|gb|EE184792.2|EE184792

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTTTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|86475140|gb|DY241510.1|DY241510

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|148936039|gb|EC880097.2|EC880097

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|78022434|gb|DV490821.1|DV490821

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|5901117|gb|AW042217.1|AW042217

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTG-CTTGG-ATCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCT

EST: gi|78106672|gb|DV525090.1|DV525090

EST:     AAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: AAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|22542657|gb|BU093095.1|BU093095

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|149101790|gb|EE187903.2|EE187903

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

EST: gi|71426546|gb|DR807596.1|DR807596

EST:     CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACA                         GTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT
genomic: CGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 67

CCAGTTAGATCCTGATGATCTTAGTGAACAATTGAAGCGGCAAGGTGCTTCGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttctt ... gtcattgcagGTTCTTAGTCGCATATCTGTCTTGGGATCTGCATTTCTGGCTGTGCTAGCTGCTGGACCATCTTTAGTTGAACAGACAAGTCACTTGACAGCATTCCGTG

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 205

Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 33

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 78

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CCAGTTAGATCCTGATGATCTTAGTGAACAATTGAAGCGGCAAGGTGCTTCGATACCACTTGTTAGACCAGGAAAAAGTACAGCTGCTTATATTAAAACAgtgagttcttacacttccatttaaatatacgcttttcttcaattctaatgcacatgtttcaactagttaccttattgctgtaaaatgtggaagttgtcca

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGCT