ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

CGAATGAGGTACCGAGTCACTAGACTGCCGAAGTATCTGATCCTTCATATGCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagattttacacttgcttgattaactttgctgtcgcctgtcatgctccatggtagctgattttaatatgttttaatattcaacttggcag

Basic information

species	Zea mays
transcript	GRMZM2G091151_T02
intron #	7
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G091151_T01 (Zea mays), 5'ss of exon 7
lower sequence: LOC_Os09g24250.1 (Oryza sativa), 5'ss of exon 7

CGAATGAGGTACCGAGTCACTAGACTGCCGAAGTATCTGATCCTTCATATGCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagattttacacttgcttgattaactttgctgtcgcctgtcatgctccatggtagctgattttaatatgttttaatattcaacttggcag
|| |||||||| |||||||  ||||| || ||||||||||||||||| |||||| ||||||||||||||||||| ||||| ||||| || |||||||||||| | ||      |||| ||   | | ||   |  |  |||   | | |  |||||  |  || ||| ||||||  ||||| | |||  |||
CGTATGAGGTATCGAGTCATCAGACTTCCAAAGTATCTGATCCTTCACATGCGACGGTTTACCAAAAATAACTTCTTTGTAGAGAAGAATCCTACTCTAGgtaaaatc-----cttgttt---tgatttaattagcctctgcagtccact-tggtacat--ttctaagatgtttacatatttatctta-cag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|31250431|gb|CD219455.1|CD219455

EST:     GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAG                         TTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA
genomic: GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagatttt ... aacttggcagTTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA

EST: gi|60348304|gb|DN215277.1|DN215277

EST:     GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAG                         TTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA
genomic: GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagatttt ... aacttggcagTTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA

EST: gi|71434280|gb|DR815330.1|DR815330

EST:     GAAGGTTTTACCAAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAG                         TTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA
genomic: GAAGGTTT-ACCAAAAA-TAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagatttt ... aacttggcagTTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA

EST: gi|76927561|gb|DV171298.1|DV171298

EST:     GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAG                         TTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA
genomic: GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagatttt ... aacttggcagTTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA

EST: gi|76927562|gb|DV171299.1|DV171299

EST:     GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAA-CCCTACTCTAG                         TTAATTTTCCTGTGAAGAACTTGGAGTTGAGGGACTACATTCCATTACCTA
genomic: GCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagatttt ... aacttggcagTTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 113

CGAATGAGGTACCGAGTCACTAGACTGCCGAAGTATCTGATCCTTCATATGCGAAGGTTTACCAAAAATAACTTTTTTGTCGAGAAAAACCCTACTCTAGgtgagatttt ... aacttggcagTTAATTTTCCTGTGAAGAACTTGGAGTTGAAGGACTACATTCCATTACCTAAGCCGAAAGAGAATCATAAGCTACGTTCAAAGTATGACCTCATAGCAAA

Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 63

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 45