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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ggccggcctatccaactttctttgacagtgataaaaggtattgcttgatgtatattttgactaaaaagagttaattacagctcaacttgaatgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC

Basic information

species Zea mays
transcript GRMZM2G074024_T01
intron # 2
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G074024_T02 (Zea mays), 3'ss of exon 2
lower sequence: LOC_Os03g40260.1 (Oryza sativa), 3'ss of exon 2
-ggccggcctatccaactttctttgacagtgataaaag-gtattgcttgatgtatattttgactaaaaagagttaattacagctcaacttgaatgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC
| || | | | || | || | || | | || | | | || | || || || | | | | | ||| | |||||| || | ||| || ||||| ||||||||||||||||| ||||||||||| || || || || ||| | ||||| ||| || |||||||||| ||||| |||||||| ||
tgagcgccagagctaagatgagttaattctgttgcacccataacagtggttttacttgttc-ctgaagaaaatcactcacatc-caacttaaaaatatgtagGGGCGAATGATTTTGGCCAGTTGGGAGACGGGACAGAGGAGAGTGCAAAGGAACCCCAAAAGGTGAAGGCACTGGAGACTGAATTTGTGAAATCAGTGTC
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|76933450|gb|DV173464.1|DV173464
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|31663993|gb|CD573173.1|CD573173
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|211129102|gb|FL371420.1|FL371420
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|29543619|gb|CB603999.1|CB603999
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|211047338|gb|FL129969.1|FL129969
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|211158187|gb|FL395436.1|FL395436
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|211019672|gb|FL390358.1|FL390358
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GNGCTAATGAGTTTGGCCAGTTGGGAGATGGG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGG
EST: gi|76021288|gb|DT948458.1|DT948458
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|71420798|gb|DR805186.1|DR805186
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
EST: gi|76930470|gb|DV172399.1|DV172399
EST:     CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGG                         GTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG
genomic: CCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 50

TTCAAATTGGGGAATGGGAAGAGCCTCAGGTGGACCGATATTGCATGTGGCCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 45

TTCAAATTGGGGAATGGGAAGAGCCTCAGGTGGACCGATATTGCATGTGGCCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 37

TTCAAATTGGGGAATGGGAAGAGCCTCAGGTGGACCGATATTGCATGTGGCCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 39

TTCAAATTGGGGAATGGGAAGAGCCTCAGGTGGACCGATATTGCATGTGGCCGTGAGCATACCGCTGCAGTTGCTTCCGATGGATCACTGTTCACCTGGGgtaagttctg ... atgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgatgtatattttgactaaaaagagttaattacagctcaacttgaatgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC
     tatattttgactaaaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ggccggcctatccaactttctttgacagtgataaaaggtattgcttgatgtatattttgactaaaaagagttaattacagctcaacttgaatgcaatcagGTGCTAATGAGTTTGGCCAGTTGGGAGATGGGACAGAGGACAGCGCGAAAGAGCCCAAGAAGGTCAAGTCATTGGAGACTGAGTTTGTAAAATCAGTATC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - -gataaaag
- - - - - - - - - - - - - - - - - - - - -tgcttga
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aaaaaga
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tacagct