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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtcattcattttgtttcaaatattccttcatttgagttttattttcacataccaagttctgatgtccttacatttttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG

Basic information

species Zea mays
transcript GRMZM2G032944_T02
intron # 3
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71765663|gb|DR963600.1|DR963600
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|78124011|gb|DV542395.1|DV542395
EST:     GCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: GCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|149085005|gb|EE174515.2|EE174515
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|71448268|gb|DR829318.1|DR829318
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|93284224|gb|EB676488.1|EB676488
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|76914812|gb|DV165698.1|DV165698
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|31355055|gb|CD439412.1|CD439412
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|71438631|gb|DR819681.1|DR819681
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTTCTTGGAGAGA
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGA
EST: gi|89761751|gb|DY689640.1|DY689640
EST:     TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTG                         ATCCCAGTCACCATGGGATTAGAGAAGAGCACCCAGTCTTCCTTGG
genomic: TTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 43 (downstream exon)
Score: 28

CACCCCTTTTTAATGGATGTTTCTAAAGAAACTTTCCCAGATTGGTTATTTTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 41

CACCCCTTTTTAATGGATGTTTCTAAAGAAACTTTCCCAGATTGGTTATTTTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG


Block sizes: 45 (upstream exon), 48 (downstream exon)
Score: 19

CACCCCTTTTTAATGGATGTTTCTAAAGAAACTTTCCCAGATTGGTTATTTTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG


Block sizes: 47 (upstream exon), 40 (downstream exon)
Score: 17

CACCCCTTTTTAATGGATGTTTCTAAAGAAACTTTCCCAGATTGGTTATTTTGCAAAGCCTGTCAAACTTCGCTGGGAAAAGCTGCTGCATCTTTACTTGgtcattcatt ... ttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tttgagttttattttcacataccaagttctgatgtccttacatttttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG
                                  tccttac  putative branch site (score: 17)
                                          tttttgctttc  CT-rich tract
      ttttattttcacata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtcattcattttgtttcaaatattccttcatttgagttttattttcacataccaagttctgatgtccttacatttttgctttcagATCCAAGTCACCATGGAATTAGAGAAGAGCACCCAGTCTTCCTTGGAGAGAACCAATGTTGTGCTGGTGGCATGGATTTTATTACCATG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CATGGA