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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC

Basic information

species Selaginella moellendorffii
transcript EFJ31718
intron # 11
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: LOC_Os05g11140.2 (Oryza sativa), 3'ss of exon 13
------------------------------------------gtattgtcgctgttattg-atggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                          ||      | |||||||  |    || || | || | | |      |||||     |||||||| | |||  | |||||||| |||||||| ||||| |||||||| ||||||||||| ||||| ||||||| ||| || || || || ||| | |||||
attagttggtgtgtacatggaaagtttgtgtgcacaggatacgttcccctgttgttattacactagca-tgtccttatagattgtaactgattttga-gcagGGACGCAATATGCCCAACAATCCTACAAAGTAAGCGACTCATTTCCCTTCAAGTGGATAAACAAGAAGTGGAAGGAGGGTTTCTATGTCACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: LOC_Os01g10150.1 (Oryza sativa), 3'ss of exon 13
-------------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                            || ||| | | ||  | |||  ||            |||||   ||| | |  | |||| || |||||  | || ||||| |||||||| || ||||||||||| ||||| ||||| ||||||||||||| ||| ||||| || || ||| || ||||
gagaagccattcacagcgcaaacacttgggtgtcttgtaatgtttactgttggtatttattatgagcaagaagccaagaactgact--tgaatgcttctcagGAACGCCATATGCACAGCAATCCTACAAAGTAAGTGATTCATTTCCCTTCAAATGGATAAACAAAAAGTGGAAGGAGGGGTTCTATGTCACTGCCTTGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: LOC_Os01g54100.2 (Oryza sativa), 3'ss of exon 12
--------------------------gtattgtcgctgttat-tgatg-gccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                          ||  | |  ||||  | | |||    | |     | |  || ||     || |      ||| |||   || || |||||||| |||||||| || ||| | || || ||||| ||||| ||||| || |||||||| || ||||| ||||||||||||||
gtaaccagttaaagcatgctgtttcagtgctattcctgtcttataatgtataaattattctttgactaacttagcatgcttttttagGAACAATGTATACACAACAATCCTACAAAGTAAGTGATACTTTCCCTTTCAAATGGATAAACAAGAAATGGAGGGACGGTTTTTATGTGACTTCCATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GRMZM2G158177_T01 (Zea mays), 3'ss of exon 6
----------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgat-tcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                        | ||  | | ||  |||| |    |     |    |  ||||  ||  | |   |  |||| |||| |||  | || || || |||||||| |||||| ||||||| ||||| ||||| ||||||||||||| ||| || || || || ||| | |||||
ttttggttctatgctgtccaaagtgggagtggtaacccttgaatatttgttgccattggtatataaataaacaagtaatgactggttctctgttctccagGAACACAATATGCCCAGCAGTCCTACAAAGTAAGCGATACATTTCCCTTCAAATGGATAAACAAAAAGTGGAAGGAGGGCTTCTATGTCACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GRMZM2G116151_T03 (Zea mays), 3'ss of exon 13
---------------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                              |  | | ||  || | |||   |  | | || |    ||||   |   ||| |  |||| |||| |||  | || ||||| |||||||| ||||| ||||| || ||||||||||| |||||||| |||| ||| || ||||| || ||| | |||||
gtcaagctgcgattgcaagcttttagctggaggctgtgacagtttatggtattgcacttgtagatacactgtagaatgga-attgac---tttgtcctctccagGAACACAATATGCCCAGCAATCTTACAAAGTAAGCGACTCATTCCCTTTCAAGTGGATAAACAAAAAATGGAAGGAGGGTTTTTATGTTACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GRMZM2G045259_T02 (Zea mays), 3'ss of exon 13
-------------------------------------gtattgtcgctgttattgatggccactggc--attgttgctgacgcgtgattcc---cccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                     | |||   | |   |    | |||        |    |  |||| |  | || |    |  |||| |||| |||  | || || || |||||||| || ||| ||||||| ||||| ||||| ||||||||||||| ||| || || || || ||| | |||||
ggaaaatgagctactcaatccccgggaatcccagaggggatttaagtttccaa-actagccctaaataaacaagtaatgactctggttttctgttctccagGAACACAATATGCCCAGCAGTCCTACAAAGTAAGTGATACATTTCCTTTCAAATGGATAAACAAAAAGTGGAAGGAGGGCTTCTATGTCACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA07G01890.1 (Glycine max), 3'ss of exon 13
-------------------gtattgtcgctgtta--ttgatggccactggcattgtt----gctgacgcgtgattccc--ccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                    ||||| |  ||| |  |||             |||||    ||||||   ||||      |  |||| ||||  ||   ||| ||||| || ||||| || || |||||||| ||||||||||| ||||||||||||||||| || |||||||||||||| |||||
gttagtacctctaaagtatatattggcaatgtcaatttggcaaatttcaattttgttttccgctgac-tatgatcttggactccagGCACACAGTATTTGCAGCAATCATATAAAGTTAGTGACTCATTTCCTTTCAAGTGGATTAACAAAAAGTGGAGGGAGGGCTTTTACGTGACTTCAATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA08G21570.1 (Glycine max), 3'ss of exon 13
--------------------gtattgtcgctgtta--ttgatggccactggcattgtt----gctgacgcgtgattccc--ccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                     ||| | |  ||| |  |||             |||||    ||||||   ||||      |  |||| ||||  ||   ||| ||||| |||||||| || ||||||||||| || |||||||| ||||||||||||||||| || |||||||||||| | |||||
gttagttcctcttaaagtatatatcgcctatgtcagtttggcaattttcaattttgttttccgctgac-tatgatcttggactccagGCACACAGTATTTGCAGCAATCATACAAAGTTAGTGATTCATTTCCTTTTAAGTGGATTAACAAAAAGTGGAGGGAGGGCTTTTACGTGACTGCAATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA06G09190.1 (Glycine max), 3'ss of exon 13
---------------------------------------------------------------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattc---ccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                                                                              |||| |  ||| |  || |    |||| | || || ||      ||  |    ||    ||| || |  |||  ||| ||||| || ||||| || ||||||||||| || ||||||||||||||||| ||||| ||||| ||||| || ||| | |||||
gttaatcccctgaagtatttattcttcatgtattgtgtctttgggatggcgataattggcttgagtttagatgatcacatgttttgcttttgcatattttgactggttatgtttaa-actgtc-ttcttacttgtatttggattgatccttttagGTACCCAGTACTTGCAGCAATCCTATAAAGTCAGTGATTCATTTCCATTTAAGTGGATCAACAAAAAATGGAGAGAAGGATTTTATGTCACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA16G04580.1 (Glycine max), 3'ss of exon 14
-------------------------------gtattgtcg-ctgttattgatggccactggcattgttg----ctgacgcgtgattcccccgc-----agGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                               | |   ||  ||||| || |       || | || |      || | |  ||| |||    |     ||| || || |||||||| || || ||||||||||| || |||||||| ||||| ||||| ||||| |||||||  ||||| ||  | || || || ||| |
tattttagctcgtttaacttctgtgatgaaaggaacatcaactgttcttcagaatttatgactttatatttatctcatgtttgaatcctttactttatagGAACTCCTTACACGCAGCAGTCCTACAAAGTGAGTGAATCATTTCCTTTCAAATGGATAAACAAGAAGTGGAAAGAAGGATTCCATGTTACCTCAATGAC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA04G09070.2 (Glycine max), 3'ss of exon 13
-------------------------------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattc---ccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                                              |||| |  ||| |  || |    | | |  || || ||      ||  |    ||    ||| || |  |||   || ||||| || ||||| || ||||| ||||| || ||||||||||||||||| ||||| ||||| ||||| || ||| | |||||
gttaatacccctgaagtttttattcttcatgtattgtgtatttggggtggcaataatatacatattttgactggttatgttta--aattgtcttcttacttgtatttggattgatccttttagGTACCCAGTACTTACAGCAATCCTATAAAGTCAGTGATTCGTTTCCATTTAAGTGGATCAACAAAAAATGGAGAGAAGGATTTTATGTCACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA04G09080.1 (Glycine max), 3'ss of exon 13
-----------------------------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattc---ccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                                           ||||| |  ||| |  || |    | | |  || || ||      ||  |    ||    ||| || |  |||   || ||||| || ||||| || ||||| ||||| || || |||||||||||||||||||| ||||| ||||| || ||| | |||||
gttaatacccctgaagtttttattcttcatgtattgtatttggggtggcaataatatacgtattttgactggttatgttta--aattgtcttcttacttgtatttggattgatccttttagGTACCCAGTACTTACAGCAATCCTATAAAGTCAGTGATTCGTTTCCATTTAAATGGATCAACAAAAAGTGGAGAGAAGGATTTTATGTCACTGCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA10G44050.1 (Glycine max), 3'ss of exon 13
----------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattc---ccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                         || ||   |||  | || |  || |||   |||||| |||    | ||         ||||| || |  || || |||||||| ||||||||||| || || || || ||||| ||||| ||||| |||||||| ||||| ||| | || ||||| |||||
gcttttgtttgaacacatggatttctgaagacctctacatataatgcttctgcaactgtta-cctctgattttgttg-tgatttattatctaatttttgcagGTACGCAGTATACTCAACAATCCTACAAAGTGAGTGAATCTTTCCCCTTCAAATGGATAAACAAGAAGTGGAGAGAAGGTTTTCATGTCACTTCAATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: GLYMA20G38770.1 (Glycine max), 3'ss of exon 13
---------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattc---ccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                        |||  |       |    |  || |||   |||||| |||    ||||       | ||||| || |  || || |||||||| ||||||||||| || || || || ||||| ||||| ||||| |||||||| ||||| ||| | || ||||| |||||
tgcttttgtttgaacacgtgggtttctgaagacctctacatataatgctcagcaactgttacctctgattttgttg-tgatttatgatctaatttctgcagGTACGCAGTATACTCAACAATCTTACAAAGTGAGTGAATCTTTCCCCTTCAAATGGATAAACAAGAAGTGGAGAGAAGGTTTTCATGTCACTTCAATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: AT2G25760.2 (Arabidopsis thaliana), 3'ss of exon 13
--------------------------gtat-tgtcgctgttattgatggccactggcattgttgctgacg-cgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                          | |  |||  | ||||||   |     |  |  | |  | |    | | |||      ||||||| |  ||    |||||||| |||||||| || ||||| ||||| ||||||||||| || ||||||||||||||||||||||| || || || |||||
gtattcttgttttactccttttgaatgcaggtgtaacagttatttccgttttatctctgtttcacagttttcatcatttgtattcagGGACCCAGTATTTACAACAATCCTACAAAGTCAGTGATTCTTTTCCCTTCAAGTGGATAAATAAAAAGTGGAGGGAAGGGTTTTATGTTACATCAATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: AT3G13670.1 (Arabidopsis thaliana), 3'ss of exon 13
--------------------------------gtattgtcgctgt----tattgatggccactggcattgttgctgac-----gcgtgattcccc---------cgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                 | | |||  | |    | ||  | | | |||   |||| ||| |      |  || ||  |           | ||| || |  || || || || || |||||||| ||||||||||| |||||||||||||| ||||| || ||||| ||||| ||  | || || || |||||
gtatctacacttttcatgaatacttttgaacactttcgtcattatggtattttactagtctctgaatttgtggcttattctatgtttggttttcaaatgaattatgaagGTACGCAGTATACACAGCAGTCTTACAAAGTAAGCGATTCATTCCCGTTCAAGTGGATAAACAAGAAATGGAGAGAAGGCTTCCATGTAACCTCAATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: AT3G03940.1 (Arabidopsis thaliana), 3'ss of exon 14
--------------------------------------gtattg--tcgctgttattgatggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                       |||||  ||        |||  |||  |    || || |     | || |    |   ||| || || || || || ||||| |||||||| ||||| |||||||| ||||||||||| || ||||||||||  ||||| ||| | || || |||||| |
gtcggtagtcacgtctcatgataactttgccaatgaacttattgattcataaagcatgaaagccgttttacttatt-ccacttcatg-taatgcgatagGAACTCCTTATACCCAGCAATCTTACAAAGTCAGCGACTCATTTCCATTCAAGTGGATAAATAAAAAGTGGAAAGAAGGTTTTCATGTAACATCCATGAC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: Vv09s0002g03680.t01 (Vitis vinifera), 3'ss of exon 13
-----------------gtattgtcgctgttattgatgg---ccactggcattgttgctga---cgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                 |  |  | | | |   |||        |     ||| |   |||   |   | |||  | | |||| |||| ||||  |||||| || |||||||| || ||||||||||| ||||||||||| || ||||| |||||||| || || || || || || |||||
gttagtctaacatatttggctgatggatttctatgacaaaatttatctatattttgcttgacttcatttcattgactc-cagGTACACAATACTTGCAACAGTCTTACAAAGTTAGTGATTCATTTCCCTTCAAGTGGATAAATAAAAAATGGAGGGAGGGTTTCTATGTCACCTCTATGGC

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 11
lower sequence: PP1S66_174V6.1 (Physcomitrella patens), 3'ss of exon 13
---------------------------------------------------------------------------------------------------------------gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgatt-cccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                                                                                                               || || | ||| || ||  |  |   | ||||  |  || ||| ||| ||    | | ||| ||  | |||||||| || || |||||||||||||| || ||||| || ||||||||||| || || ||| | ||||| |||||||||||||| |||||
gtattttattggatagtatcctttgatgtatattgaatctattttcagagttgatctcaagggtgtttgatgatttattcttataatttgtcatttggagacctttttgtggttttctggctcttgtt--ttacttgttgcatgatgtctcacg-gtgtttatttctgtagGAACGTCGTACACGCAGCAGTCTTACAAAGTGAGCGACTCTTTTCCATTTAAGTGGATCAATAAGAAATGGCGAGAAGGCTTTTACGTGACTTCAATGGC

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|169079095|gb|FE488619.1|FE488619
EST:     TGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCGTGTCAAAAG                         GGACACCGTACACGCAACAATCATACAAAGTGAGCGATTCATTTCCGTTCA
genomic: TGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCATGTCAAAAGgtattgtcgc ... tcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCA
EST: gi|169077785|gb|FE485463.1|FE485463
EST:     TGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCATGTCAAAAG                         GGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCA
genomic: TGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCATGTCAAAAGgtattgtcgc ... tcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCA
EST: gi|169051034|gb|FE458204.1|FE458204
EST:     TGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCATGTCAAAAG                         GGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCA
genomic: TGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCATGTCAAAAGgtattgtcgc ... tcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 64

GAGTGGATCATGGAACAATGGGAGAAAAACTTCTACATTAGTGCCATTGCAGGAGCCAACAACGGCAGCTCGCTGGTTGTCATGTCAAAAGgtattgtcgc ... tcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgtcgctgttattgatggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC
                               tgctgac  putative branch site (score: 64)
                                            ttcccccgc  putative PPT
        ttattgat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtattgtcgctgttattgatggccactggcattgttgctgacgcgtgattcccccgcagGGACACCATACACGCAACAATCGTACAAAGTGAGCGATTCATTTCCGTTCAAGTGGATCAACAAAAAGTGGAGGGAAGGGTTTTACGTGACTTCCATGGC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGTGGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGG