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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacgaaaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG

Basic information

species Physcomitrella patens
transcript PP1S61_57V6.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: LOC_Os01g68950.1 (Oryza sativa), 3'ss of exon 1
---gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacgaaaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
   | |     | || | ||         | | ||       |||  |   | | |      |  | || ||  |  | | || ||    |  |||| ||||||||||    ||||| |||||  | ||||| ||  || |     ||   |||||||||||||| || |||||||| ||||||||||||       ||||||
catgatattttggttatgcagcactctcgaatgagaaattatttgaca-atagt-tgtgtttttttgtgctcgattcgttaac-aatttcctttgttctgtagGATGGGAACGAGGTATTCTTCCGCATCAAGAGATCTACGCAGCTGAAGAAGCTGATGAACGCCTATTGTGACCGTCAGTCTGTGGATATGAATGCTATTG

upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: LOC_Os01g68940.1 (Oryza sativa), 3'ss of exon 1
gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacgaaa------aatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
              | | | |||    | |   |   | |   |    | || |  | ||  |||||| || | ||     ||       | ||  |||  ||||||||||    |||||||||||| | ||||| ||  |  ||    ||   |||||||||||||| |||||||| |||||||| ||||||      |||||||
-----tgtttgcgatgatgagatgttgccagattttttttcttttgttagtaaagtgcattttatgttttg-gcgccataaaaccttatcattccttgtgctgtagGATGGCAACGAGGTGTTCTTTCGTATCAAGAGATCAACCCAACTGAAGAAGCTGATGAACGCCTACTGTGATCGCCAGTCCGTGGATATCAAATCTATTG

upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: GRMZM2G082390_T02 (Zea mays), 3'ss of exon 1
gttggcacgctttttcaagggataaaactaaggtgcctgttggtat-gcaatatacagaataatatgttctgtgagc--tgacga-----aaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
  |  || |   | |  |     |||  | |    | | ||| ||| ||||| |  ||  | || |||    |||    |  | |     | ||| |   || | ||||||||    |||||||||||| | || || ||  |  ||    ||   ||||||||||||||||| ||||||||||||||||||||        | ||||
--ttacatgtcatattga-----aaaggttacacactt-ttgatatcgcaatctcaagtgttatctgtcacttgactcttttccatctttataataataattgtatagGATGGCAATGAGGTGTTCTTTCGCATAAAGAGGTCCACCCAGCTGAAGAAGCTGATGAACGCTTATTGTGACCGCCAGTCTGTGGACATGAATGCCATTG

upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: GRMZM2G053898_T02 (Zea mays), 3'ss of exon 1
gttggcacgctttttcaagggataaaactaaggtg----cctgttggtat-gcaatatacagaataatatgttctgtgagctgacga-----aaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
          |||| || |  |||    ||||||      || ||| |||  || | |  ||  | || |||    |||     | |     | ||| |   || | ||||||||    |||||||||||| | || || ||  |  ||    ||   |||||||||||||| || || |||||||||||||||||        | ||||
----------ttttacatgtcatattgttaaggttacactcttttgatatcacattctcaagtgttatctgtcacttgactcttccatctttataataatatttatatagGATGGCAATGAGGTGTTCTTTCGCATAAAGAGGTCCACCCAGCTGAAGAAGCTGATGAACGCCTATTGCGACCGCCAGTCTGTGGACATGAATGCCATTG

upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: AT5G55160.1 (Arabidopsis thaliana), 3'ss of exon 1
gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacga------aaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
||||| ||    ||     || || |  ||  ||   || |  |  | ||     | | |||||   |   |||  | || |        |||   | || ||||||||||    || || |||||||||||||| || |   |     |    || |||||||| |||||||||||||| ||||||||||||     ||| ||||
gttggtacttggtt-----ggttatagatacagtttttggtattgagaaaatcgtata-taatactcttcttgaagtaacaatcattggtaatttttcttgtgtagGATGGTAATGAAGTCTTCTTTAGGATCAAGAGAAGCACTCAGCTCAAAAAACTGATGAATGCTTACTGTGACCGTCAGTCTGTGGATTTCAACTCAATTG

upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: AT5G55160.2 (Arabidopsis thaliana), 3'ss of exon 2
gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacga------aaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
                                ||   || |  |  | ||     | | |||||   |   |||  | || |        |||   | || ||||||||||    || || |||||||||||||| || |   |     |    || |||||||| |||||||||||||| ||||||||||||     ||| ||||
--------------------------------gtttttggtattgagaaaatcgtata-taatactcttcttgaagtaacaatcattggtaatttttcttgtgtagGATGGTAATGAAGTCTTCTTTAGGATCAAGAGAAGCACTCAGCTCAAAAAACTGATGAATGCTTACTGTGACCGTCAGTCTGTGGATTTCAACTCAATTG

upper sequence: PP1S61_57V6.1 (Physcomitrella patens), 3'ss of exon 1
lower sequence: AT4G26840.1 (Arabidopsis thaliana), 3'ss of exon 1
--gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacgaaaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
    |  || | |  | | |      |  |    |     |||  | |   |||| | |  |   | |||   ||  |   | | |  |   |||||| |||||||||   ||||| |||||||||||||| || |   |     |    ||||||||||| |||||||||||||| || ||||||||      ||| ||||
actctttcaggttactaccaaaagagagccatgtgatttagttattgtttcatatgcggtctctaacgtttattgttccttctatatgtt--tgttttatagGATGGAAACGAGGTTTTCTTTAGGATCAAGAGAAGCACTCAGCTCAAGAAGCTGATGAATGCTTACTGTGACCGGCAATCTGTGGACATGAACTCCATTG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|162272448|gb|FC448255.1|FC448255
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162253843|gb|FC430152.1|FC430152
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162238524|gb|FC414610.1|FC414610
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|18328994|gb|BJ161000.1|BJ161000
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|67692582|gb|BJ952815.1|BJ952815
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162201142|gb|FC377649.1|FC377649
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162167758|gb|FC344072.1|FC344072
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|67569322|gb|BJ942146.1|BJ942146
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|18356302|gb|BJ188361.1|BJ188361
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162273907|gb|FC450046.1|FC450046
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162267422|gb|FC443965.1|FC443965
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|100413413|gb|BY956659.1|BY956659
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162270077|gb|FC446777.1|FC446777
EST:     CGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: CGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|208355471|gb|DC938816.1|DC938816
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACCGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162238525|gb|FC414611.1|FC414611
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|37833234|gb|BJ591246.1|BJ591246
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|208344666|gb|DC905115.1|DC905115
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|67575717|gb|BJ948541.1|BJ948541
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162216231|gb|FC392648.1|FC392648
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|18361093|gb|BJ193157.1|BJ193157
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162267423|gb|FC443966.1|FC443966
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|18337202|gb|BJ169226.1|BJ169226
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|67699199|gb|BJ959432.1|BJ959432
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
EST: gi|162216232|gb|FC392649.1|FC392649
EST:     AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAG                         GATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG
genomic: AACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 35 (upstream exon), 35 (downstream exon)
Score: 90

GGGGGTGGAGGACAGCAGCAACCCGGGTGTGCAGCATCAGGACGAGAAGAAACCTCTCGACGGAGCAGGGCAGCACATCAATCTCAAGGTCAAGGGCCAGgttagtagct ... tgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgcaatatacagaataatatgttctgtgagctgacgaaaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG
                            agctgac  putative branch site (score: 90)
     tatacagaataatat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttggcacgctttttcaagggataaaactaaggtgcctgttggtatgcaatatacagaataatatgttctgtgagctgacgaaaaatcagtgttttgtagGATGGAGGGGAGGTGTTCTTTAGGATCAAAAGCACTGCCACTTTGCGCAAGCTGATGAACGCTTACTGTGACCGCCAGTCTGTGGATCCTTCCTCTATTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGAT