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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gttagtcaattttattcgagtgactactgagtactgactggacaagactttttccttggacggtgcttatgttgttttaccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCCCCTTCTAGCAATGGGGACGCTAAGGAGCCAACTGAATCACAAGACAACG

Basic information

species Oryza sativa
transcript LOC_Os01g66520.1
intron # 12
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88585671|gb|CI703327.1|CI703327
EST:     GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
genomic: GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
EST: gi|88589158|gb|CI707017.1|CI707017
EST:     GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
genomic: GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
EST: gi|86482980|gb|CI125613.1|CI125613
EST:     GCGCTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
genomic: GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
EST: gi|89166141|gb|CI103231.1|CI103231
EST:     TCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGCGGTGAGAACAACTCAATAAATCCATCC
genomic: TCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
EST: gi|33797724|gb|CF324721.1|CF324721
EST:     GCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
genomic: GCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
EST: gi|88584733|gb|CI702306.1|CI702306
EST:     GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
genomic: GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC
EST: gi|86535377|gb|CI175583.1|CI175583
EST:     GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAG                         AACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCGATAAATCCATCC
genomic: GCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 50 (upstream exon), 71 (downstream exon)
Score: 3

GATTCTGCTGAGGTGCCTGATTGCACTCTTGCTTCAAAAGATTCAAATGAACCTGAAACTTTTGCCAAAGAGgttagtcaat ... taccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCCCCTTCTAGCAATGGGGACGCTAAGGAGCCAACTGAATCACAAGACAACG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

actgactggacaagactttttccttggacggtgcttatgttgttttaccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCCCCTTCTAGCAATGGGGACGCTAAGGAGCCAACTGAATCACAAGACAACG
 tactgac  putative branch site (score: 3)
                                          ttttaccct  putative PPT
                                       ttgtttta  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gttagtcaattttattcgagtgactactgagtactgactggacaagactttttccttggacggtgcttatgttgttttaccctgcagAACGAAACGAGCACAAGTTGCAGTGGTGAGAACAACTCAATAAATCCATCCCCTTCTAGCAATGGGGACGCTAAGGAGCCAACTGAATCACAAGACAACG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AAGGAG