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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtaagtagctctttcgcctccctcgtcctaatctgccaatttttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG

Basic information

species Selaginella moellendorffii
transcript EFJ31718
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 4
lower sequence: GLYMA04G09080.1 (Glycine max), 3'ss of exon 5
------------------------------------------------------gtaagtagctctttcgcctccctcgtcctaatctgccaatttttttaa----cagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG
                                                      || |       | | |  |  ||     | || |     |  ||||      ||| |||||||||||||||||||| ||||||||  | |||||||| || ||| | || || ||||||||  | ||                            
gtgagtatctatctgaaatgatattgaaatgtaatgcatcttatttttcttaatgttatagagaatattgaatagctaacttttatttctttttccttttgggtttcagGATGTCTACTGAAATGGTTGCATGTATTGCAATCGAGGCTATCTCCATATTAGAGAAGATGCATTCTAGAGG----------------------------

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 4
lower sequence: GLYMA06G09190.1 (Glycine max), 3'ss of exon 5
-------------------------gtaa-gtagctctttcgcctccctc---------gtcctaatctgccaatttttttaa----------cagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG
                         |||| | | ||  ||   ||   |           |  | |   || | | |||||            ||| |||||||||||||||||||| ||||||||  | |||||||| || ||| | || || ||||||||  | ||                            
gtgagtatctctgaaatgttgaaatgtaatgcatcttatttttcttaatgttatagagaatattgaatagcgactctttttccttttgggtttcagGATGTCTACTGAAATGGTTGCATGTATTGCAATTGAGGCTATCTCCATATTAGAGAAGATGCATTCTAGAGG----------------------------

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 4
lower sequence: PP1S66_174V6.1 (Physcomitrella patens), 3'ss of exon 5
------------------------------------------------------gtaagtagct-ctttcgcctccctcgtcctaatctgccaatttttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG
                                                        |   |  | ||||   ||          | |  |   || ||| |  ||| ||||| || |||||||| ||||||||||| |||||| ||||||| || ||||| |||||||| |   | ||                            
gtaagtgacgctctattgacaggcttttcgcttgaataatgtggccttcgatcgacattcaattactttttactgtgaaactgaagttcgattatctttctg-cagGATGTCCACCGAAATGGTGGCCTGTATTGCCGTGGAGTCTATTTCAATCCTTGAGAAAATGCACTGTAGAGG----------------------------

upper sequence: EFJ31718 (Selaginella moellendorffii), 3'ss of exon 4
lower sequence: PP1S131_16V6.1 (Physcomitrella patens), 3'ss of exon 5
-----------------------------------------------gtaagtagctctt-tcgcctccctcgtc------ctaatctgccaattttttt--aacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG
                                               |  || | |  || || ||     |          | |||    |||||||||    ||| ||||| ||||||||||| |||||||| || |||||| |||| || || ||||| |||||||| |   | ||                            
gtaagtatttactaccctgatgttctcttcgtctattattactgctggcgaggatcgtttatcaccaattgcagagaaaaattgatcccgtaattttttttttgcagGATGTCAACTGAAATGGTGGCCTGTATAGCCGTGGAGTCTATCTCAATCCTTGAGAAAATGCACTGTAGAGG----------------------------

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|169096720|gb|FE496850.1|FE496850
EST:     GACATGCTTGGGCCTAGCTTGTGGGATGTGTGGAACTCGTCAGGACAAGC                         CATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
genomic: GACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
EST: gi|169114703|gb|FE516900.1|FE516900
EST:     GACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGC                         CATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
genomic: GACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
EST: gi|169036770|gb|FE443808.1|FE443808
EST:     GACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGC                         CATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
genomic: GACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
EST: gi|169104490|gb|FE503774.1|FE503774
EST:     GACATGCTTGGGCCTAGCTTGTGGGATGTGTGGAACTCGTCAGGACAAGC                         CATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
genomic: GACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
EST: gi|169066653|gb|FE475435.1|FE475435
EST:     GCTTGTGGGATGTTTGGAACTCGTCAGGACAAGC                         CATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
genomic: GCTTGTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
EST: gi|169094739|gb|FE491577.1|FE491577
EST:     GTGGGATGTTTGGAACTCGTCAGGACAAGC                         CATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT
genomic: GTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 34 (upstream exon), 34 (downstream exon)
Score: 27

CTCGAGTGCACCATAAGGGGCGTCAAGGAGATTACTACGTAATGGTTATGGACATGCTTGGGCCTAGCTTGTGGGATGTTTGGAACTCGTCAGGACAAGCgtaagtagct ... tttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

gtaagtagctctttcgcctccctcgtcctaatctgccaatttttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG
                             tcctaat  putative branch site (score: 27)
                                           ttttttt  putative PPT
                                         aatttttttaaca  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtaagtagctctttcgcctccctcgtcctaatctgccaatttttttaacagCATGTCTACTGAAATGGTTGCCTGTATTGCGGTGGAGGCTATTTCGATACTTGATAAAATGCATTCGCGGGGCTATGTTCACGGAGATGTGAAGCCAGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGAT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGATG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC