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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagctgattccgttctctgcttaacaatttccaaatccaattttctcctgcagtccgtaatctctaaaatctgtggcctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG

Basic information

species Zea mays
transcript GRMZM5G859691_T01
intron # 8
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G859691_T01 (Zea mays), 3'ss of exon 8
lower sequence: PP1S337_21V6.2 (Physcomitrella patens), 3'ss of exon 4
-------------------------------------------------------------------gtgagctgattccgttctctgcttaacaa--tttccaaatccaattttctc-ctgcagtccgtaatctctaaaatctgtggcctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG
                                                                   |||    | |   ||  |   | |   ||  |||     ||    |      |||    | ||  |   | |    ||          ||||||||||||| |||   ||||||||||||| |||||| || || ||||||||||| ||| | || || ||||||||  | |   | ||| |||| |||||
gtgagtgatgggagaaggggtgaggtgaatgcagcatgtgcgttggttgcgaaagtggtggaggtgtgtggagaggtggggtgtttgacatgggaaggtttgtgggtcggggtaagaaactgagagcagttgtgg-tgatgaatgcaatgcaatgcagGTATCGTGCTGGACAGTGGAGATGGTGTGACCCACACGGTGCCAATCTACGAGGGTTACGCACTGCCTCACGCCATATTGCGATTAGACTTGGCGGGTCG
















RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 33 (upstream exon), 49 (downstream exon)
Score: 53

GAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCCGCTATGTACGTCGCCATCCAGGTCGTGCTCTCTCTGTATGCCAGTGGTCGTACCACAGgtgagctgat ... cctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG


Block sizes: 17 (upstream exon), 47 (downstream exon)
Score: 16

GAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCCGCTATGTACGTCGCCATCCAGGTCGTGCTCTCTCTGTATGCCAGTGGTCGTACCACAGgtgagctgat ... cctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG


Block sizes: 10 (upstream exon), 49 (downstream exon)
Score: 8

GAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCCGCTATGTACGTCGCCATCCAGGTCGTGCTCTCTCTGTATGCCAGTGGTCGTACCACAGgtgagctgat ... cctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG


Block sizes: 30 (upstream exon), 49 (downstream exon)
Score: 124

GAGAAGATGACCCAGATCATGTTTGAGACCTTCAACACCCCCGCTATGTACGTCGCCATCCAGGTCGTGCTCTCTCTGTATGCCAGTGGTCGTACCACAGgtgagctgat ... cctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

caaatccaattttctcctgcagtccgtaatctctaaaatctgtggcctctctcagGTATCGTGCTCGACTCGGGAGATGGTGTGAGCCACACCGTCCCCATCTACGAGGGATACACCCTCCCCCACGCCATCCTTCATCTTGACCTGGCCGGTCG
                              ctctaaa  putative branch site (score: 124)
                                             cctctctc  putative PPT
       aattttct  TA-rich tract