ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
PPT, BS, UA-tract

Sequence

atgc intronic sequence ATGC exonic sequence

gtagtttatgccacctattatcaatactgtaagtagaggtatgtcctgctcttagacttcttatcctaaaatgttgactatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA

Basic information

species	Zea mays
transcript	GRMZM2G468479_T01
intron #	11
splice site	3'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G468479_T01 (Zea mays), 3'ss of exon 11
lower sequence: LOC_Os01g09760.1 (Oryza sativa), 3'ss of exon 10

gtagtttatgccacctattatcaatactgtaagtagaggtatgtcctgctcttagacttcttatcctaaaatgttgactatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA
   ||  |  |||||  | |||||| ||   ||||  |  |     ||   | |       ||  || || | | |  ||  ||||||| | ||  |||| ||| || |||||||||||  |||||||||||||||| |||||||||||||| ||||| |||||||||||||||||||||    |||
---gtacac-ccaccattcatcaatgct---agtat-gccacagattgagttcaa---ctgtagtcttaattctggcttactttcagGCTAGGAGATTGCAGCATTGCTTGTCATCTGAGTCACTTCGTAGATGGTGTACATATGAGTGGTTTTACAGCGCAGTTGATTATCCATGGTTTATGGATA

upper sequence: GRMZM2G468479_T01 (Zea mays), 3'ss of exon 11
lower sequence: GLYMA20G37820.1 (Glycine max), 3'ss of exon 11

--------------gtagtttatgccacctat----tatcaatactgtaagtag----agg-----tatgtcctgctcttaga------cttcttatcctaaaa-tgttgact---------atgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA---
               || ||||       |||    | |  |  || | ||  |    | |     | | | |  ||  ||||      ||| || | |||||| |  | |||         |  | |||||||||  ||| ||    || ||| ||||| |     |||| |||||||  |  | ||| ||||| ||||||||| ||||||||||||||||| | || |   
gtacaagctttttcttaatttaaattgattatgtgtttttgaagctttgaggggcttcaagctttttgtttacaacttatagaaaaaagctttttgttctaaaaataataacttcctctataacttgcagGCAAAG--GTTTCTA-GTTGCTTGGCATCTAATATGGTTCGCAGATGGTTTATTTTTGAATGGTTTTACAGTGCAATTGATTATCCATGGTTTGCCAAAAGGG

upper sequence: GRMZM2G468479_T01 (Zea mays), 3'ss of exon 11
lower sequence: AT3G21430.2 (Arabidopsis thaliana), 3'ss of exon 12

---------------------------------------------------gtagtt-tatgccacctattatcaatactgtaagtagaggtatgtcctgctcttagacttc-----ttat-cctaaaatgttgactat-gttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTT-TCAAATA--
                                                   | |||  ||   |    | | | || |   | | |     |      |  |||  || |||     ||||   || |   |   ||||  ||| |    |||||||   ||| ||  | ||   |   | |   || |||||||| |  | ||||||||||||||||||| |||| ||||||||||||  || | |  
gtgagtgaactttctgatactcagactttattcccaaatgataaagaaaccgcagtgatacatctttcaatcttaagatctttaatttctatggaatttagtctgtgagttccctgattatatttataccatatcctattattctgcagGAGAAGTTTTCGCATTGCATTTCTAGTTTCCGAGCACGGAGATGGTGTATTTTTGAGTGGTTCTACAGTGCAATTGACTATCCATGGTTTGCCAGACAAG

upper sequence: GRMZM2G468479_T01 (Zea mays), 3'ss of exon 11
lower sequence: AT5G27610.1 (Arabidopsis thaliana), 3'ss of exon 11

gtagtttatgccacctattatcaatactgtaagtagaggtatgtcctgctcttagacttcttatcctaaaatgttgactatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA---
         |  |     || |   | | |   || |   ||    |     | | || |||||       || | | |   || |     ||||||||    | ||| |||||| |  |    | ||| ||||||||| |||||| ||||||||||||||  |||| ||||| |||||| |||| |   
---------gtgagtctctaacttcatttttcttatacaaatcaaataaatctggtct-cttatt------tgcttattccatttaca-gGAGAAGTTGTCAAATTGTCTGTCATATCCATTGGTACGTCGATGGTGCATATATGAATGGTTCTACAGTGCTATTGACTATCCCTGGTTTGCAAAGATGG

upper sequence: GRMZM2G468479_T01 (Zea mays), 3'ss of exon 11
lower sequence: Vv05s0020g01610.t01 (Vitis vinifera), 3'ss of exon 10

gtagtttatgccacctattat---caatactgtaagtagaggtatgtcctgctcttagacttcttatcctaaaatgttgactatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA---
   ||| |       | ||||   || | || |||     |||   |     | | |   || || |    | || |  | | |  |      || |  || ||  | ||  | || |   | |   | || ||||||||  | | ||||||||||||||||||| ||||||| ||||||||| | || |   
---gttaaaattttttgttatgtccatttctataatgtttggttccttaaagtttaaatatttttgt----atatataaattttaattgcagGAAAAGCTTTCTA-ATTGCCTATCTTGCTATCGGGTACGGAGATGGTGTGCTTTTGAGTGGTTCTACAGTGCAATTGATTACCCATGGTTTGCTAAAAAGG

upper sequence: GRMZM2G468479_T01 (Zea mays), 3'ss of exon 11
lower sequence: Vv14s0128g00880.t01 (Vitis vinifera), 3'ss of exon 2

--gtagtttatgccacctattatcaatactgtaagtagaggtatgtcctgctcttagacttcttatcctaaaatgttg-----actatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTT-TCAAATA--
    | |||    |  |  | |    | | | || |  || ||| |         |       |  | |||| ||||      ||| | ||     ||  |  || ||    |  || |||||| |       |||||||||||||| |  |||||||||||||||||| ||||||| || |||||| | ||| |  
gttgaatttgcatcttccttcactttttcagaaacttcagttatctaagatataaaataaagctgacataaattgtttttttcactgtatttgtagAAGAAGATTTCT-TGTTCCTTATCATCTTACATGGCACGTAGATGGTGCACTTTCGAGTGGTTCTACAGTGCAATTGATTACCCTTGGTTTGTTAAAAAAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|32930123|gb|CF034935.1|CF034935

EST:     AAAGCCTATGTCGTGCAATGAAGGTTCAGATAATCTTCAG                         GCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGC
genomic: AAAGCCTATGTCGTGCAATGAAGGTTCAGATAATTTTCAGgtagtttatg ... ctatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 35

CAAGCCTGAATTTATAATGTCTGGAAGGACCAGGTTGAAATCTCAGATGCATTGCAAAACAAAGCCTATGTCGTGCAATGAAGGTTCAGATAATTTTCAGgtagtttatg ... ctatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA

Block sizes: 43 (upstream exon), 44 (downstream exon)
Score: 20

Block sizes: 41 (upstream exon), 47 (downstream exon)
Score: 18

Block sizes: 46 (upstream exon), 44 (downstream exon)
Score: 23

atgc intronic sequence ATGC exonic sequence

Intronic sequence truncated to 55 bases.

gtagaggtatgtcctgctcttagacttcttatcctaaaatgttgactatgttcagGCAAAGAAGTTGCTGCACTGTTTGTCATCTGAACCACTTCGTAGATGGTGCACATATGAGTGGTTCTACAGTGCAGTTGATTATCCATGGTTTTCAAATA