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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...ttatgtgcatactttagtctctgtgcgcaaatctttgaatatatgggaatctgaccacagctactaattgtgggccctttatgatttaatattttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG

Basic information

species Zea mays
transcript GRMZM2G387076_T02
intron # 5
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G387076_T02 (Zea mays), 3'ss of exon 5
lower sequence: LOC_Os01g64650.2 (Oryza sativa), 3'ss of exon 4
----ttatgtgcatactttagtctctgtgcgcaaatctttgaatatatgggaatctgaccacagctactaattgtgggccctttatgatttaatattttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG
    |||| | |     | | |   | | | || | |||    |  ||  |  |   ||  |   | || || |||     |||||| | |||||||| ||||||||||||||||||||||||| ||    ||||| || ||||||||||||||||||||||| || || || |||||||||||||| |||
catattatctactgttatgaattgatat-ctcataacttatttttcatatgggtaaaactgc--ttgctgat-gtgcattgtttatgttctaatatttgaacagCATAAAACTATTGAGAGTGTGCTCCAAAGAGGGGAAAGATTGGATAGCTTGGTTGAGAAGAGTTCAGACCTGAGTGCTGCTTCACAG

upper sequence: GRMZM2G387076_T02 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA09G13840.1 (Glycine max), 3'ss of exon 4
ttatgtgcatactttagtctctgtgcgcaaatctttgaatatatgggaatctgaccacagctactaattgtgggccctttatgatttaatatttt--aacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG
|| ||| |||   ||  |        |      || |  | | |||   ||||      |    |  || |   |   | |||  ||  | |||   ||||||||||||||||||| ||||| || || ||||| ||||   ||||||| || |||||||| || |||||||||||||| ||||| |||
ttttgttcatttcttcctacacaattggcttgtttaggttctctggttttctggtgt--gtagatttttttcaccttatgatgtctttctgtttataaacagCATAAAACTATTGATAGTGTGCTAGCTAGAGGGGAGAAGCTGGATAGTTTAGTTGAGAAGAGTTCTGATCTGAGTGCCGCTTCGCAG

upper sequence: GRMZM2G387076_T02 (Zea mays), 3'ss of exon 5
lower sequence: GLYMA13G19950.1 (Glycine max), 3'ss of exon 4
ttatgtgcatactttagt-ctctgtgcgcaaatctttgaatatatgggaatctgaccacagctactaattgtgggccctttatgatttaatatttt--aacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG
 | ||| |||   ||  | | |  |  ||   | || |  | | | |   ||||      |    |   | |   |   | |||  ||  | |||   ||||||||||||||||||| ||||| || || ||||| ||||   |||| || || |||||||| || |||||||||||||| || || |||
ctttgttcatttcttcctgcacaataggcttgt-ttaggttctctagttttctggtgt--gtagatctctttcaccttatgatgtctttctgtttataaacagCATAAAACTATTGATAGTGTGCTGGCTAGAGGGGAGAAGCTGGACAGTTTAGTTGAGAAGAGTTCTGATCTGAGTGCCGCATCGCAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|74235349|gb|DT643263.1|DT643263
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
EST: gi|211529456|gb|FL249093.1|FL249093
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTTGAGAGTGTCC
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATT-GAGAGTGTCC
EST: gi|211460687|gb|FL127589.1|FL127589
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAARACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
EST: gi|211529460|gb|FL249097.1|FL249097
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
EST: gi|60354098|gb|DN221071.1|DN221071
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
EST: gi|149057998|gb|EE156679.2|EE156679
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
EST: gi|211529454|gb|FL249091.1|FL249091
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTGAGAGTGTCCTTGCCAGAGGAG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAG
EST: gi|44681914|gb|CK786962.1|CK786962
EST:     ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTA                         CATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG
genomic: ACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 42 (upstream exon), 43 (downstream exon)
Score: 53

GATCCTGCGGAAGCTGACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG


Block sizes: 43 (upstream exon), 49 (downstream exon)
Score: 60

GATCCTGCGGAAGCTGACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 60

GATCCTGCGGAAGCTGACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG


Block sizes: 48 (upstream exon), 43 (downstream exon)
Score: 65

GATCCTGCGGAAGCTGACAAATTAATGAAAATTCAGAGGGACTTGGATGAAACAAAAATTATCCTAgtaagtgtga ... attttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ggaatctgaccacagctactaattgtgggccctttatgatttaatattttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG
                                tttatgatttaatatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ttatgtgcatactttagtctctgtgcgcaaatctttgaatatatgggaatctgaccacagctactaattgtgggccctttatgatttaatattttaacagCATAAAACTATTGAGAGTGTCCTTGCCAGAGGAGAGAGATTGGATAGCTTGGTTGAGAAAAGCTCTGATCTGAGTGCTGCTTCTCAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGCTGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGCT
- - -tgcatac
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -cagctac