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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gaaaattctttctcaccttgtaactgtttggccttttttctagcaaaggaagaaataactcatgacctttcatattttgtatattcatgtgtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA

Basic information

species Zea mays
transcript GRMZM2G151015_T02
intron # 3
splice site 3'
intron type U2

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 91

GTGCTCGTCGTGGCCACAAGCGTGTTGTCCCCCGACGCAGAGAAGAGgtcgggcatg ... gtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 134

GTGCTCGTCGTGGCCACAAGCGTGTTGTCCCCCGACGCAGAGAAGAGgtcgggcatg ... gtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 149

GTGCTCGTCGTGGCCACAAGCGTGTTGTCCCCCGACGCAGAGAAGAGgtcgggcatg ... gtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA


Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 118

GTGCTCGTCGTGGCCACAAGCGTGTTGTCCCCCGACGCAGAGAAGAGgtcgggcatg ... gtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aaggaagaaataactcatgacctttcatattttgtatattcatgtgtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA
       aaataac  putative branch site (score: 118)
 tgttttt  putative PPT
 tttcatattttgtata  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gaaaattctttctcaccttgtaactgtttggccttttttctagcaaaggaagaaataactcatgacctttcatattttgtatattcatgtgtttttacagAATTGTTGCGGTAGATGCAACCGAAGAGGGTCTTCAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAGG
- - - - - - - - - - - actgttt
- - - - - - - - - - - - - - - - - - - - - -caaaggaa
- - - - - - - - - - - - - - - - - - - - - - - - - - aaataac
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - attcatg