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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...aatagtggtgtcttggatgaagtagtactgctgttgtaatattgaattgcaattgattccatatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA

Basic information

species Zea mays
transcript GRMZM2G145578_T01
intron # 4
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G145578_T01 (Zea mays), 3'ss of exon 4
lower sequence: LOC_Os12g31830.1 (Oryza sativa), 3'ss of exon 4
-------aatagtggtgtcttggatgaagtagtactgctgttgtaatattgaattgcaattgattcca-tatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA
       | || |  ||   | | |||  |  |   |        |  | |  ||||| |||||  |  ||||||||||       | ||| ||   |   ||||||||||||||| | ||||| |||||||| |||||||||||  | ||||||||||||||||||||||||||||| ||||||||||| |||  |||||| |||||
gattgaaactaatactgcaatag-tgatatcatggagtgagacatacgtcgggttgcagttgataacgctatatgtgat-------ggtgtatttatttatttatcagGTGATACTGTCGTTGCAGTGGATAGTCCTTTTGGACGACTTGGGCTTACTGTTTGCTATGATTTGAGATTTCCAGAGCTTTACCAATGTTTGCGCTTTAA

upper sequence: GRMZM2G145578_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA10G43950.1 (Glycine max), 3'ss of exon 4
aatagtggtgtcttggatgaagtagtactgctgt-tgtaatattgaattgcaattgattccatatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA
|  |||| |     |     |  || ||   ||  | | |||| |||     |  |||    | |   ||     || |  ||||||     | || |||||  |   ||||||||| || || || ||| |||| || ||||| || | ||||||||||||||||||||| |||||| | || ||| |  |||||||  |
aggagtgtttgaaagagaagataagcacacttggatatgatat-gaaccatgaaggatggtgttttcatgtgaaattcttctgttttggtgatgttttcagGCAAGGATATTGTTGCAGTAGACAGTCCTGTTGGGCGTTTGGGCCTCAGTGTTTGCTATGATTTGAGATTTCCAGAGATGTACCAATTGCTGCGTTTCCA

upper sequence: GRMZM2G145578_T01 (Zea mays), 3'ss of exon 4
lower sequence: GLYMA20G38690.1 (Glycine max), 3'ss of exon 6
aatagtggtgtcttggatgaagtagtactgctgt-tgtaatattgaattgcaattgattccatatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA-
    ||| |     |     |  || ||   ||  | | |||| | |     |  |||      |   ||     || |  ||||||     | || |||||    | ||||||||| || || || ||| ||||||| ||||| || | ||||||||||||||||||||| |||||| | || ||| |  | |||||  | 
----gtgtttgaaagagaagataagcacacttggatatgatat-gcaccatgaaggatggggctttcatgtgaatttcttctgttttggggatgttttcagGCTGGA-TATTGTTGCAGTAGACAGTCCTATTGGACGTTTGGGCCTCAGTGTTTGCTATGATTTGAGATTTCCAGAGATGTACCAATTGCTACGTTTCCAA

upper sequence: GRMZM2G145578_T01 (Zea mays), 3'ss of exon 4
lower sequence: Vv13s0067g03530.t01 (Vitis vinifera), 3'ss of exon 4
-aatagtggtgtcttggatgaagtagtactgctgttgtaatattgaattg-caattgattccatatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA
   |  |||||| |   | | | ||||     | |   |  ||||||| |   |||       | | ||||  ||   ||  |   ||       || |||||  |     ||||||| || || |||||  |||| || |||||  | || |||||||||||||||||||||||||||||||||||     |  | ||   
tttttctggtgtttcccaagtaatagttgccttatgacataattgaatggatgatttgacttttttgtgtg--tgagaattctaaattggtgaagttttcagGGAAGGAAGTTGTTGCGGTAGACAGCCCCATTGGTCGTTTGGGTGTAACGGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAGCAGCTAAGGTTCCT

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|71773229|gb|DR971138.1|DR971138
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|93013637|gb|EB639157.1|EB639157
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|149099913|gb|EE185966.2|EE185966
EST:     CGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: CGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|211174534|gb|FL157623.1|FL157623
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|91877591|gb|EB407548.1|EB407548
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTTGACGGTTGGGGGCT-ACT
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGG-CTTACT
EST: gi|149099914|gb|EE185967.2|EE185967
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|32937778|gb|CF042597.1|CF042597
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|101385197|gb|EB814644.1|EB814644
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|67026709|gb|CO455458.1|CO455458
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|5296166|gb|AI783041.1|AI783041
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|148930223|gb|EC872658.2|EC872658
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|211148755|gb|FL046692.1|FL046692
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|78078129|gb|DV506544.1|DV506544
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|71435450|gb|DR816500.1|DR816500
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|22490063|gb|BU049986.1|BU049986
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAG
EST: gi|50328228|gb|CO523354.1|CO523354
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|148928133|gb|EC872657.2|EC872657
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|60359149|gb|DN226122.1|DN226122
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|93013636|gb|EB639156.1|EB639156
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATATTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|76910593|gb|DV163937.1|DV163937
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|87152344|gb|DY397133.1|DY397133
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|78180385|gb|DV550758.1|DV550758
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|60346599|gb|DN213572.1|DN213572
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|211174535|gb|FL157624.1|FL157624
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCA-                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|6695954|gb|AW289062.1|AW289062
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|76016655|gb|DT943825.1|DT943825
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|71773228|gb|DR971137.1|DR971137
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|6127314|gb|AW129839.1|AW129839
EST:     CGTTTCCCAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: CGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|76294351|gb|DV033919.1|DV033919
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
EST: gi|12058143|gb|BF733058.1|BF733058
EST:     TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAG                         GTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG
genomic: TGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 112

GTTTGATGTTGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 193

GTTTGATGTTGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 215

GTTTGATGTTGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA


Block sizes: 49 (upstream exon), 45 (downstream exon)
Score: 154

GTTTGATGTTGATGTACCTGGCAATATGGTGTACAAGGAAAGTCGTTTCACAGCCGCAGgtgattattt ... ctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

attgcaattgattccatatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA
     aattgat  putative branch site (score: 154)
                                   tgttttctctctctta  putative PPT
     aattgatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
aatagtggtgtcttggatgaagtagtactgctgttgtaatattgaattgcaattgattccatatatgtgattgcttatgatgttttctctctcttatcagGTGATACTATTGTTGCTGTGGATAGCCCTTTTGGACGGTTGGGGCTTACTGTTTGCTATGATTTGAGATTCCCAGAGCTTTATCAAATTTTGCGTTTTAA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  AGAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GAGCTT
- - - - - - ttggatg
- - - - - - - - - - - - - - -gctgttg
- - - - - - - - - - - - - - - - - - - - - - aattgca
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgattgc