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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...acaaatatcttctttttcgattggtccacaaagttaatcacatccaattaagtttgttggtatctaattgctgaatccagtggccctttacgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC

Basic information

species Zea mays
transcript GRMZM2G112782_T01
intron # 3
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60359241|gb|DN226214.1|DN226214
EST:     ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|110194413|gb|EC876311.1|EC876311
EST:     ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|149070488|gb|EE158891.2|EE158891
EST:     ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|71442014|gb|DR823064.1|DR823064
EST:     ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|14204198|gb|BG837875.1|BG837875
EST:     ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|110193128|gb|EC875026.1|EC875026
EST:     TGATATGTCATATAATCTTGGCCTCAGGGGTTGTATCCTTTTTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: TGATATGTCATATAATCTTGACCTCA-GGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|60337811|gb|DN204784.1|DN204784
EST:     CTTGACCTCAGGGTTGTATCTTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: CTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
EST: gi|211054682|gb|FL184554.1|FL184554
EST:     ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAG                         AGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG
genomic: ATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 96

TTGGAATAGCTTGTGGAAATCCAAGAGGGATGAACAACGCCCATTAAGATATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 37

TTGGAATAGCTTGTGGAAATCCAAGAGGGATGAACAACGCCCATTAAGATATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 27

TTGGAATAGCTTGTGGAAATCCAAGAGGGATGAACAACGCCCATTAAGATATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 159

TTGGAATAGCTTGTGGAAATCCAAGAGGGATGAACAACGCCCATTAAGATATGATATGTCATATAATCTTGACCTCAGGGTTGTATCCTTATTAACACAGgtatatcgat ... cgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aattaagtttgttggtatctaattgctgaatccagtggccctttacgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC
                atctaat  putative branch site (score: 159)
                                      ccctttac  putative PPT
               tatctaatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
acaaatatcttctttttcgattggtccacaaagttaatcacatccaattaagtttgttggtatctaattgctgaatccagtggccctttacgttttacagAGATTTAATGGGCTAGCGGAGAGAAGCAGAAATTATTTGACAAGTTGGAAGGAAAATTCCAGGACTTCTGGAAGGACGGGTGGGCTTCATATACAAGGCC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGGAAG
- - - - - - - - - - - - -ccacaaa
- - - - - - - - - - - - - - - - - - - - catccaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - tgttggt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - tgctgaa