Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC

Basic information

species Zea mays
transcript GRMZM2G053027_T01
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: LOC_Os08g42640.1 (Oryza sativa), 3'ss of exon 1
--tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC
  | | | |     | ||| || ||  || |  |||  || || ||||||| | | ||    | |   ||| | | | |||||| ||   ||| | ||  ||| || ||||| || ||||| ||||||||||| || |||||||||||||| |||||||||||||||||||| ||||||||||| |||||||| |  || ||
catgtattcatagcctcatctgtgtacatcttcatgcatgtat-aaaaataaactttggcgcccttcacaggtggttaaactaa-cattttttccctgttagACTGCATTGGATCGGCATTTAATGCAAAAGGGGCCATGCAATGCCCTAATTGTCGGAAGATTGAGAAAGGCCGCTGGCTTTACGCAAGTGGGCACCATCC

upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA20G38050.2 (Glycine max), 3'ss of exon 1
tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacat-tatgata-tcttaagcagattgctgaactaagcaaaatttt---cttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC
         |||   || |  |  ||     || | |   ||| ||  | || |   ||| |  ||  |  ||   |   || |  |  | |||    |    |||||| ||||| || ||||||||   ||||||||| ||||| ||||| || ||||||||||||||||||||||  ||||| ||||| | |||  |||   |
-----gatgtcatgaatttttgcatacactgatttcttattgacaacaatatatgcaatagtaatattcttatgcttatgcttattattactttacattatttagATTGCATTGGTTCGGCATTCAACATAAAAGGAGCAATGCAGTGCCCCAATTGTCGGAAGATTGAGAAAGGTCAATGGCTATATGCTAATGGTTGTCGGTC

upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: GLYMA10G29230.1 (Glycine max), 3'ss of exon 1
-tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgc---cagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC
    ||    |||   || |  |  ||     || | |   ||  ||  | || |||  || |||     |||  |   || |  |  | |||         |||||| ||||| || ||||||||   ||||||||| ||||| |||||||| ||||||||||||||||||||||  ||||| ||||| | |||  ||| | |
aatagtgatgtcatgaatttctgcatacactgatttcttgttggtaacaatat-atggaatattag---cattcttatgcttattattactttacaatatttagATTGCATTGGTTCAGCATTCAACATAAAAGGAGCAATGCAGTGCCCTAATTGTCGGAAGATTGAGAAAGGTCAATGGCTATATGCTAATGGTTGTCGCTC

upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: AT3G05545.1 (Arabidopsis thaliana), 3'ss of exon 1
tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaa--aattttcttgcc----agATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC
  | ||| |  |     |    || ||    ||| || ||||     |   |||||   | || |  |        ||||   ||     |||  |||      |||||||||||| || || ||||||||||| |||| |||||||||||| |||||||||||  | || ||||| |  |||||||||||||  ||  |||   |
attttcctcagattagggattattcatt---atgctt-catggttggtttcttatgt--ttttgacaaagccatctaacttcacatctgttttctttgtttttaagATTGTATTGGTTCGGCTTTCAATGCAAAGGGAGTGATGCAATGCCCCAACTGTCGGAAAGTAGAAAAAGGCCAGTGGCTTTATGCAAACGGTTGTCGTTC

upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: AT2G47700.1 (Arabidopsis thaliana), 3'ss of exon 1
tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgc---cagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC-
  |        |   | |   | |  |   | | | ||  ||   |||   ||  | ||| ||  ||       | | |         |||  || |   ||||||| ||||| || ||||| ||    |||||||| |||||||||||||||||||| ||  | || || ||||  ||| | |||||||  ||     || | 
-gtagatatagaatgaggcttgctagttctattatctggctgctgaatttgtt--gctattttcggctttccctttagcagcttgttttttggtttcttgcagATTGCATTGGATCAGCATTTAACATGAAAGGAGCCATGCAATGCCCTAACTGTCGCAATGTCGAAAAGGGTCAGTGGTTGTATGCAAACGGTT-CTACTCG

upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: Vv14s0060g02280.t01 (Vitis vinifera), 3'ss of exon 1
tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatat--cttaagcagattgctgaactaagcaaaatttt-cttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC
    ||     |   ||    | |||     | || |  |  |     | ||| |  | |  |   | |  |||  ||   | |     ||  | ||  |||||||| ||||| || |||||||||   ||||| || |||||||| ||||| |||||||| ||||| |||||||  ||||||||||||| |||  |||   |
---atcattctattaatacttgattagtgatacgtatctagcacttgcatattgtcctttggccataacttattcttgtgattattgtcataatgctctccagATTGCATTGGTTCAGCATTCAATATCAAAGGGGCAATGCAATGTCCTAATTGTCGGAAAATTGAAAAAGGTCAATGGCTTTATGCAAATGGTTGTCGTAC

upper sequence: GRMZM2G053027_T01 (Zea mays), 3'ss of exon 1
lower sequence: Vv07s0005g00420.t01 (Vitis vinifera), 3'ss of exon 1
--------tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC-
         ||||    | |   ||      | |||| || |||    | |   ||   | ||||||        |  ||  |   || |     || |  |   ||||||| ||||| ||||||||||||   ||||| || ||||||||||| ||||| || |||||||||| |||||| ||| | | ||||| ||| |    | | 
tatgttttactgttttgtaaaatataactaatcatgtaaaggttgtggtcattgatttttaatgata--------aacttagttctcttattgttatgtattatacagATTGCATTGGTTCTGCATTCAATATGAAAGGGGCAATGCAATGCCCCAACTGCCGAAAGATTGAGAGAGGTCGGTGGTTATTTGCAAATGG-CTCTGCTCG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211386049|gb|FK987343.1|FK987343
EST:     CGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAG                         ATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACT
genomic: CGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACT
EST: gi|211386047|gb|FK987341.1|FK987341
EST:     CGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAG                         ATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACT
genomic: CGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACT
EST: gi|31360282|gb|CD444639.1|CD444639
EST:     CGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAG                         ATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACT
genomic: CGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 148

GCCGGGGCCGCGGCGTGCTCCATCTGCCTGGATCCGGTGCTCGCCTGCGTCGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 111

GCCGGGGCCGCGGCGTGCTCCATCTGCCTGGATCCGGTGCTCGCCTGCGTCGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 108

GCCGGGGCCGCGGCGTGCTCCATCTGCCTGGATCCGGTGCTCGCCTGCGTCGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 64

GCCGGGGCCGCGGCGTGCTCCATCTGCCTGGATCCGGTGCTCGCCTGCGTCGCGGGCCGGTCCGTCGCCAAGCTGCAATGCGGACACGAGTTCCACCTAGgtcagattcc ... ttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

aatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC
                                           ttttcttgcc  CT-rich tract
     attatgatatcttaa  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tctgtcccctcacccatgtgcgtttatgtgaatgtttgcatgaaaaatacattatgatatcttaagcagattgctgaactaagcaaaattttcttgccagATTGTATTGGGTCTGCATTCAATGCAAAAGGAGCGATGCAATGCCCTAACTGTCGGAAGATTGAGAAAGGTCGCTGGCTTTATGCAAGTGGACGTCACCC

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AGTGGA
- - - - - - - catgtgc
- - - - - - - - - - - - - -gtgaatg
- - - - - - - - - - - - - - - - - - tgcatga
- - - - - - - - - - - - - - - - - - - - - - aaataca
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgctgaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aagcaaa