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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...tcagcatttgtttccagattcttttactttgttgatgtcagttcttgagtttccttttgctgaactagtacttatttgccaccttttctcttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT

Basic information

species Zea mays
transcript GRMZM2G048455_T02
intron # 2
splice site 3'
intron type U2

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211115832|gb|FL357315.1|FL357315
EST:     AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAG                         CTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
genomic: AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
EST: gi|47962771|gb|CN845480.1|CN845480
EST:     AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAG                         CTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
genomic: AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
EST: gi|211329270|gb|FL352880.1|FL352880
EST:     AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAG                         CTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
genomic: AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
EST: gi|211222841|gb|FL345049.1|FL345049
EST:     AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAG                         CTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
genomic: AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
EST: gi|211302625|gb|FL366318.1|FL366318
EST:     AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAG                         CTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
genomic: AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
EST: gi|211523399|gb|FL360214.1|FL360214
EST:     AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAG                         CTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC
genomic: AGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 168

CGTTTACTTGGTTTATGAGCTCATGGACACTGATCTGCATCAAATAATTAAGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 157

CGTTTACTTGGTTTATGAGCTCATGGACACTGATCTGCATCAAATAATTAAGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 234

CGTTTACTTGGTTTATGAGCTCATGGACACTGATCTGCATCAAATAATTAAGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 178

CGTTTACTTGGTTTATGAGCTCATGGACACTGATCTGCATCAAATAATTAAGTCATCTCAACCACTATCCAATGACCACTGCCAGTATTTCCTTTTTCAGgtaagtttcg ... ttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tgagtttccttttgctgaactagtacttatttgccaccttttctcttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT
                                           tcttgac  putative branch site (score: 178)
                         cttatttgccaccttt  CT-rich tract
                       tacttattt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
tcagcatttgtttccagattcttttactttgttgatgtcagttcttgagtttccttttgctgaactagtacttatttgccaccttttctcttgactgtagCTGCTCCGAGGCCTGAAGTACCTCCATTCAGCCGGGATACTCCACAGAGACCTAAAGCCAGGGAACCTCCTGGTCAACGCAAACTGTGACCTGAAGATAT

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGAAGA
- -gcatttg
- - - - - - - - - - - - - - -tgttgat
- - - - - - - - - - - - - - - - - - - - gttcttg
- - - - - - - - - - - - - - - - - - - - - - - - - - - - -tgctgaa