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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

gtgagtgcccgaggagtttacaactaatgcacagtctctaactctctatgactcatgccaggtgtttatgcactaaccgtatacattctttgtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGGAACAGTCCAACAGCCATCCGGGTGCTGAAATCTGCACTCAATGCAGCTG

Basic information

species Oryza sativa
transcript LOC_Os01g47350.1
intron # 7
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os01g47350.1 (Oryza sativa), 3'ss of exon 7
lower sequence: AT1G60550.1 (Arabidopsis thaliana), 3'ss of exon 7
---------gtgagtg--cccgaggagtttacaactaatgcacagtctctaactctctatgactcatgccaggtgttt-atgcactaaccgtatacattctttgtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGGAACAGTCCAACAGCCATCCGGGTGCTGAAATCTGCACTCAATGCAGCTG
            |||   ||| |     ||   ||  || ||||      || ||  | |   |  ||  |  |||||  | |  | || |    |  | ||| ||  |  |||| |  |||||||||  |||||| |||||||||||| || |||||||  ||||||||||||| || ||||| || || ||  | ||||| ||||||| ||
gtaaattttaccagtaaaccccatagtgttgtcacagatacacaa---aaaaatcagtctatttgttggtaattgttttgttccttcactgagacctatttttctgt-tccagCTAGAGGATTTGGAGAAAGAAACTGTGAAATGGTGCAGAGAAATCTTACGGAACAGTCCAACTGCAATCCGAGTACTTAAGGCAGCACTTAATGCAGTTG
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|29668522|gb|CB664797.1|CB664797
EST:     ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCA                         CTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
genomic: ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
EST: gi|58675867|gb|CK064554.1|CK064554
EST:     AGAATGGGTCTCGTCAATGTAGTAGTACCA                         CTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
genomic: AGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
EST: gi|29656537|gb|CB652812.1|CB652812
EST:     ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCA                         CTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
genomic: ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
EST: gi|38479469|gb|CF963584.1|CF963584
EST:     ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCA                         CTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
genomic: ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
EST: gi|29668603|gb|CB664878.1|CB664878
EST:     ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCA                         CTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
genomic: ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
EST: gi|29668562|gb|CB664837.1|CB664837
EST:     ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCA                         CTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG
genomic: ACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 1 RNA-Seqlibrary.


Block sizes: 74 (upstream exon), 75 (downstream exon)
Score: 13

GTAGGACCAAAGAAAGCTCGTGAGATGTGGTTTCTGTCACGGTTCTACACAGCTGATGAGGCAGACAGAATGGGTCTCGTCAATGTAGTAGTACCAgtgagtgccc ... gtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGGAACAGTCCAACAGCCATCCGGGTGCTGAAATCTGCACTCAATGCAGCTG




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 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

tatgactcatgccaggtgtttatgcactaaccgtatacattctttgtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGGAACAGTCCAACAGCCATCCGGGTGCTGAAATCTGCACTCAATGCAGCTG
                        cactaac  putative branch site (score: 13)
                                       ttctttgt  putative PPT
                                 tatacatt  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gtgagtgcccgaggagtttacaactaatgcacagtctctaactctctatgactcatgccaggtgtttatgcactaaccgtatacattctttgtgcatatagCTCGCTGATTTGGAGCGAGAAACGGTGAAATGGTGCCGAAAAATCTTGAGGAACAGTCCAACAGCCATCCGGGTGCTGAAATCTGCACTCAATGCAGCTG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCAGCT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CAGCTG