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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...gacgctctaattagctatgatttctgtattatcggttgtatttgattctgcacttggatatgttattcacacaaacaaacttgttctattttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCAGCGGAAGATTCGGCAAAGTATTGCTGATGAG

Basic information

species Oryza sativa
transcript LOC_Os01g21180.1
intron # 1
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: LOC_Os01g21180.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GRMZM2G180990_T01 (Zea mays), 3'ss of exon 1
-gacgctcta--attagctatg-atttctgtattatcggttgtatttgattctgcacttggatatgttattcacacaaacaaacttgttctattttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCAGCGGAAGATTCGGCAAAGTATTGCTGATGAG
 | ||   ||  || | ||| |     || | |||||| ||||     ||  |    |  | | |   ||  ||  |  |    ||| | | ||  |||| |||||||||||  |||||||||||||  ||||||||||||||||| ||||| || || ||||||||||||||||| |||||||||
aggcgacttacaatcatctaggcgaggcttttttatcgtttgttgccaatctttg--tcagttttaacatggacttagtct--tttgatattttacatctccagGTTCTCTTCAGAAGATATATCTGGACAGAATCAAGTTAAGGCTTCTGTACAACGAAAGATTCGGCAAAGTATCGCTGATGAG

upper sequence: LOC_Os01g21180.2 (Oryza sativa), 3'ss of exon 1
lower sequence: GLYMA04G41130.2 (Glycine max), 3'ss of exon 1
gacgc-tctaattagctatgatttctgtattatcggttgtatttgattctgcacttggatatgttattcacacaaacaaacttgt-tctattttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCAGCGGAAGATTCGGCAAAGTATTGCTGATGAG
||    | |  |     |||    || |||  |  || ||||   ||| |    ||   | |   ||    |     | | ||   | |   ||   | ||| || ||   ||||||| ||||||| ||||| ||||| ||||| ||||| || || || || ||||||||||||||||| |||
gatatatgtgctgcatcatgcacactctatatttcgtggtatc--attataattttaatttttcaatatttatctttagatttacatgtcagtttcttccagATTTTCAGGTGAAGATGTATCTGCACAGAACCAAGTGAAGGCATCTGTTCAACGAAAAATACGGCAAAGTATTGCTGAAGAG

upper sequence: LOC_Os01g21180.2 (Oryza sativa), 3'ss of exon 1
lower sequence: Vv14s0060g01920.t01 (Vitis vinifera), 3'ss of exon 1
gacgctctaattagctatgatttct---gtattatcg--gttgtatttgattctgcacttggatatgttatt-cacacaaacaa-acttgttctattttatctt-cagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCAGCGGAAGATTCGGCAAAGTATTGCTGATGAG
        |||| | | ||  ||||   ||||||      ||||   |  || || |     | || |   |  | ||     | | || || | || |||  |  |||||||| | ||||||| ||||  | || |||||||||||||| || ||||| || || ||||||||||| ||||| ||||||
--------aatttgatgtgcattctcttgtattactataattgtgcctatttatgtatgccaaaatttctctataaacttttgatatttcttttcttgtattctatagGTTCTCATCTGAAGATGTATCCTCACAAAATCAAGTTAAGGCATCGGTGCAACGCAAAATTCGGCAAAGCATTGCAGATGAG

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|88361922|gb|CI682096.1|CI682096
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88664198|gb|CI673276.1|CI673276
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88326211|gb|CI660024.1|CI660024
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88727487|gb|CI751142.1|CI751142
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|33688443|gb|CF316682.1|CF316682
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTGAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88692766|gb|CI736341.1|CI736341
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88584551|gb|CI702113.1|CI702113
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|33812764|gb|CF332270.1|CF332270
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88732421|gb|CI755598.1|CI755598
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88581529|gb|CI698523.1|CI698523
EST:     TCTCCTCCGCTTTAATCGCACCGNCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88582517|gb|CI699934.1|CI699934
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88573361|gb|CI664932.1|CI664932
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88329462|gb|CI648521.1|CI648521
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|218488231|gb|FG951457.1|FG951457
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATG-TCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88347712|gb|CI655065.1|CI655065
EST:     TTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88388325|gb|CI627864.1|CI627864
EST:     GCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: GCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88726864|gb|CI750519.1|CI750519
EST:     TCTCCTCCGCTTTAATCGCACCGNCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88732153|gb|CI757001.1|CI757001
EST:     TCTCCTCCGCTTTAAGCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|163928391|gb|EG714711.1|EG714711
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|55412623|gb|CV724999.1|CV724999
EST:     AATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: AATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88726544|gb|CI750199.1|CI750199
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAACATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88741938|gb|CI764443.1|CI764443
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88376867|gb|CI639192.1|CI639192
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|87012993|gb|CI297220.1|CI297220
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88405694|gb|CI609593.1|CI609593
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|25804409|gb|CA760370.1|CA760370
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|33665495|gb|CF296462.1|CF296462
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|58598583|gb|CK009111.1|CK009111
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGGCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88359324|gb|CI679851.1|CI679851
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88306080|gb|CI635086.1|CI635086
EST:     TTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88421818|gb|CI611697.1|CI611697
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|66917257|gb|CX104105.1|CX104105
EST:     TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAGAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TCTCCTCCGCTTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
EST: gi|88324957|gb|CI644968.1|CI644968
EST:     TTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAA                         GTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA
genomic: TTTAATCGCACCGCCGGCCGCCGGCGACCATGTTCAAAAAgtgagtcggc ... ttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCA


 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ttctgcacttggatatgttattcacacaaacaaacttgttctattttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCAGCGGAAGATTCGGCAAAGTATTGCTGATGAG
                                      ttctattttatcttc  CT-rich tract
                                   ttgttctattttat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
gacgctctaattagctatgatttctgtattatcggttgtatttgattctgcacttggatatgttattcacacaaacaaacttgttctattttatcttcagGTTCTCTTTTGAAGATATATCTGCGCAGAATCAAGTTAAGGCCTCTGTGCAGCGGAAGATTCGGCAAAGTATTGCTGATGAG

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCAAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGAT