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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

...acttcaatcaaaacttagatcatatgcatttatgtaacagtggtcctcgtttttttcttgcagggaagttaatgattttgtatgtttccttgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA

Basic information

species Zea mays
transcript GRMZM2G049347_T01
intron # 10
splice site 3'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G049347_T01 (Zea mays), 3'ss of exon 10
lower sequence: LOC_Os08g20544.1 (Oryza sativa), 3'ss of exon 9
---acttcaatcaaaac-ttagatcatatgcatttatgtaacagtggtcctcgtttttttcttgcagggaagttaatgattttgtatgtttccttgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA
     |  ||| |  |  ||   |  | |   |||  | || | | |       ||      | |   |  || || | ||||| ||| ||   | ||   ||||| |||||||||||||| || |||||||  |  || || ||||||||| | |||||||||||  ||||||||||  ||||||| ||||||||||| ||||
aatgttaaaataatcatgtttctttgtctatcttttcgcaataattggatagaattgggaaattccttggggtgaa-gcttttgaatgatt---tattacacagGTACACCTGGAGCTCCTGTATTACCCTTTTGGTAAACAGGAAGGGGTTTCCAATCCTTTTGCAGATCAGATTCAGTTAACTTCTTTGGAGAAGGTCCTCA
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|33092748|gb|CF052742.1|CF052742
EST:     ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT
EST: gi|211290677|gb|FL187015.1|FL187015
EST:     ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT
EST: gi|60398772|gb|DN231588.1|DN231588
EST:     GACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: GACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT
EST: gi|32794245|gb|CD946481.1|CD946481
EST:     TCTTGTGAAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: TCTTGTG-AAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT
EST: gi|211392050|gb|FL249966.1|FL249966
EST:     ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT
EST: gi|32824459|gb|CD964181.1|CD964181
EST:     GTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: GTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT
EST: gi|32932662|gb|CF037474.1|CF037474
EST:     ATCTTGTGAAGGACCTGNAAATTCAACGAGACAAGAAACCTCGTGGTCAG                         GTTCACCTGGAGCTCCTCTACTACCCTTACGCTAAGCATGAAGGGGTTCCT
genomic: ATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 150

AGTAGACTTAGCTGATATACAGCCAGGAAAAGTGAAGGATTTATGGTTAGATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 182

AGTAGACTTAGCTGATATACAGCCAGGAAAAGTGAAGGATTTATGGTTAGATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 247

AGTAGACTTAGCTGATATACAGCCAGGAAAAGTGAAGGATTTATGGTTAGATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA


Block sizes: 47 (upstream exon), 47 (downstream exon)
Score: 232

AGTAGACTTAGCTGATATACAGCCAGGAAAAGTGAAGGATTTATGGTTAGATCTTGTGAAGGACCTGGAAATTCAACGAGACAAGAAACCTCGTGGTCAGgtcagctgat ... tgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA




 atgc   intronic sequence     ATGC   exonic sequence

Intronic sequence truncated to 55 bases.

ctcgtttttttcttgcagggaagttaatgattttgtatgtttccttgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA
                     agttaat  putative branch site (score: 232)
                                     tgtttccttgttcttc  putative PPT
                       ttaatgattttgtat  TA-rich tract
















Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
acttcaatcaaaacttagatcatatgcatttatgtaacagtggtcctcgtttttttcttgcagggaagttaatgattttgtatgtttccttgttcttcagGTTCACCTGGAGCTCCTCTACTACCCTTACGCGAAGCATGAAGGGGTTCCTAATCCTTTTGCTAATCAGATTCAACTAACTTCATTGGAGAAGGTTCTCA

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TTGGAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GGAGAA
- - -aatcaaa
- - - - - - - - - - - -atgcatt
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -atgtttc