ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GAGGTGCTGCTTCCAGGTGGCCATGGCCTCATGGTTCGTGGGTATCGTCCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAAgtacgcatatctatccttttcttgttaatttggtaatcactagttgtggtttcacttccaccagccattcctagatcactgatcctgttctttgttcttcag

Basic information

species	Zea mays
transcript	GRMZM2G000052_T01
intron #	9
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G000052_T01 (Zea mays), 5'ss of exon 9
lower sequence: LOC_Os04g53190.1 (Oryza sativa), 5'ss of exon 9

GAGGTGCTGCTTCCAGGTGGCCATGGCCTCATGGTTCGTGGGTATCGTCCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAAgtacgcatatctatcc---ttttcttgttaatttggtaatcactagttgtggtttcacttccaccagccattcctagatcactgatcctgttctttgttcttcag
||||| |||||||| ||||||||||| |||||||||||||| |||||||| || ||||||||||| ||||||||| |||||||||||||   ||| | ||| ||||||| ||     ||||| |  | | ||   || ||  | |  ||||| ||  |||||||  | ||  | | | |   |   | || | | ||||| ||
GAGGTACTGCTTCCTGGTGGCCATGGTCTCATGGTTCGTGGATATCGTCCAGTAATAAATACTCTGGCAAAAGGCCTAGATATACGCCTTGGCCACAGgtatgcatatccatttagtttttccttctcactttaaaagcaaaattcatggttcca--tccaccattccttatt-gctaatccacattattttcttttctttag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|60356234|gb|DN223207.1|DN223207

EST:     CCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAA                         GGTTGTGGAAATTGTTCGCCACAGGAACAGGGTAGAGGTTACCGTAAGCAG
genomic: CCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAAgtacgcatat ... tgttcttcagGGTTGTGGAAATTGTTCGCCATAGGAACAGGGTAGAGGTTACCGTAAGCAG

EST: gi|71764900|gb|DR962837.1|DR962837

EST:     CCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAA                         GGTTGTGGAAATTGTTCGCCATAGGAACAGGGTAGAGGTTACCGTAAGCAG
genomic: CCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAAgtacgcatat ... tgttcttcagGGTTGTGGAAATTGTTCGCCATAGGAACAGGGTAGAGGTTACCGTAAGCAG

EST: gi|14242894|gb|BG840215.2|BG840215

EST:     CCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAA                         GGTTGTGGAAATTGTTCGCCATAGGAACAGGGTAGAGGTTACCGTAAGCAG
genomic: CCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAAgtacgcatat ... tgttcttcagGGTTGTGGAAATTGTTCGCCATAGGAACAGGGTAGAGGTTACCGTAAGCAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 88

GAGGTGCTGCTTCCAGGTGGCCATGGCCTCATGGTTCGTGGGTATCGTCCGGTTATAAATACTCTCGCAAAAGGCTTAGATATACGCCTCAACCATAAgtacgcatat ... tgttcttcagGGTTGTGGAAATTGTTCGCCATAGGAACAGGGTAGAGGTTACCGTAAGCAGCGGCCAAACGTTTGTTGCGGATGCTGCTGTGGTCACTGTTCCGTTGGGT

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 91

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 58