Skip to another splice site:
1  
 5'  3'   		2  
 5'  3'   		3  
 5'  3'   		4  
 5'  3'   		5  
 5'  3'   		6  
 5'  3'   		7  
 5'  3'   		8  
 5'  3'   		9  
 5'  3'   

Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaaatggaactttaaaatttaatatcgtcacaaacaaatcaatttttgtatcagacataatggcaaacacattaaatgaacaacacaaacact...

Basic information

species Zea mays
transcript GRMZM5G814159_T01
intron # 8
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G814159_T01 (Zea mays), 5'ss of exon 8
lower sequence: LOC_Os06g35030.3 (Oryza sativa), 5'ss of exon 8
GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaaatggaactttaaaatttaatatcgtcacaaacaaatcaatttttgtatcagacataatggcaaacacattaaatgaacaacacaaacact-
||| |||| ||||||||||| ||||||||||||||||||||||||||||| |||||||||||||| ||||||||| |||  | || ||| |  || ||||| |  |   |   |  |  | |  | | |    || ||  ||   |    |||   |||       ||  
GTTGCTCGACTGAGGCTACTGGGTGATTATGTGCACTCCACATGCATCGCGTTTGTTGAGTTTGTTCAAgtaagtaaaacagtaaattttactttaaatattgcaagttaatgagaatgtatcattttatgt-tattgaaaagtgctgctaattgtcaaagaggttacca
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32911170|gb|CF015982.1|CF015982
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTTCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|78180693|gb|DV551066.1|DV551066
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|148929870|gb|EE285590.2|EE285590
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGTTTTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|6827601|gb|AW331244.1|AW331244
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|31363320|gb|CD447677.1|CD447677
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|12046152|gb|BF728291.1|BF728291
EST:     TTGGTGATTATGTGCACTCCCCATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|67020345|gb|CO449094.1|CO449094
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|32860477|gb|CF000159.1|CF000159
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|71772178|gb|DR970115.1|DR970115
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|110932361|gb|EE152587.1|EE152587
EST:     TTGGGGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGTTTTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|18164011|gb|BM333850.1|BM333850
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|32835281|gb|CD974959.1|CD974959
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|31909455|gb|CD650927.1|CD650927
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|14242646|gb|BG840348.2|BG840348
EST:     TGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|211513327|gb|FL030343.1|FL030343
EST:     TTGGTGATTACGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|211122862|gb|FL458470.1|FL458470
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|6851486|gb|AW352496.1|AW352496
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|110190841|gb|EC872739.1|EC872739
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTTTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|33091830|gb|CF051824.1|CF051824
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|76292409|gb|DV031977.1|DV031977
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|86467992|gb|DY234362.1|DY234362
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|211513328|gb|FL030344.1|FL030344
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|32911777|gb|CF016589.1|CF016589
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|211375738|gb|FL457579.1|FL457579
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|32165016|gb|CD670334.1|CD670334
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|14243113|gb|BG840833.2|BG840833
EST:     CCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: CCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|18173999|gb|BM349387.1|BM349387
EST:     TTGGTGATTATGTGCACT-CACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|14243826|gb|BG841499.2|BG841499
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|86467993|gb|DY234363.1|DY234363
EST:     TTGGAGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|32937185|gb|CF042004.1|CF042004
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|37395891|gb|CF635231.1|CF635231
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|67021641|gb|CO450390.1|CO450390
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|149099389|gb|EE185429.2|EE185429
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTTTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|31362976|gb|CD447333.1|CD447333
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
EST: gi|16926655|gb|BM079723.1|BM079723
EST:     TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAA                         GCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG
genomic: TTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 392

GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACGCTTCCTGTTAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 251

GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACGCTTCCTGTTAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 180

GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACGCTTCCTGTTAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 502

GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaa ... gtttatgcagGCAGATAGTGCTATTCTCGCTCTGAACTGCAGTGGCATTGTTCTTGGAACGCTTCCTGTTAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTTTCTCGTCTGAGGCTACTTGGTGATTATGTGCACTCCACATGCATCGCTTTTGTTGAGTTTGTCCAAgtaagttaaaatggaactttaaaatttaatatcgtcacaaacaaatcaatttttgtatcagacataatggcaaacacattaaatgaacaacacaaacact

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - acaaacaa
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - aacacaaa