ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CGCATTCAGATGGATAACCTTACTGCTGACCCAGGAATTCAACTTCGCCGACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcgagcgcgcacactgtttcttctgctctggaattatctttggctctgtttctttgcctaaactttgtctgctgaacacag

Basic information

species	Zea mays
transcript	GRMZM2G068392_T01
intron #	8
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G068392_T01 (Zea mays), 5'ss of exon 8
lower sequence: LOC_Os01g68010.3 (Oryza sativa), 5'ss of exon 8

CGCATTCAGATGGATAACCTTACTGCTGACCCAGGAATTCAACTTCGCCGACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcgagcgcgcacactgtttcttctgctctggaattatctttggctctgtttctttgcct-aaactttgtctgctgaacacag
||||||| | ||||| ||  ||||| | |||||||| |||||||| || ||||| |||||||| ||||||||||||||||||||||||| ||| || |||||      | |     ||| ||| ||  ||    |  |  ||   |||   ||   ||| | |||| ||||||| ||||| ||   |||
CGCATTCCGGTGGATTACGCTACTGTTAACCCAGGAGTTCAACTTTGCTGACACTATTCACATATGGGACACACTATTAAGTGATCCTGATGGTCCTCAGgtagcaaacaaa---acaccctggtttatcc-tgcaaggcttggtttt---tccagttcgtcgcctgaaactttatctgccgagtgcag

upper sequence: GRMZM2G068392_T01 (Zea mays), 5'ss of exon 8
lower sequence: GLYMA05G28660.1 (Glycine max), 5'ss of exon 8

CGCATTCAGATGGATAACCTTACTGCTGACCCAGGAATTCAACTTCGCCGACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcgagcgcgcacactgtttcttctgctctg---gaattatctttggctctgt--ttctttgcctaaactttgtctgctgaacacag-------
 ||||| || ||||| ||  | |||||||| ||||||||||| || ||||||| | ||||||| ||||| || || ||||| ||||| | ||| || |||||        |     || | |    ||| | || ||   || ||   ||| |   |||  | |  |||||    | |     |   | | ||       
TGCATTTAGGTGGATTACTCTCCTGCTGACTCAGGAATTCAATTTTGCCGACAGCCTTCACATTTGGGATACTCTTTTAAGCGATCCAGATGGTCCACAGgtacaaatgtacaattcatatttaaccttgtactatgatcgacttgcatttagacttgtggtccactgcctcttgtataaaaacaatataaagctacttt

upper sequence: GRMZM2G068392_T01 (Zea mays), 5'ss of exon 8
lower sequence: GLYMA08G11760.1 (Glycine max), 5'ss of exon 8

CGCATTCAGATGGATAACCTTACTGCTGACCCAGGAATTCAACTTCGCCGACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcgagcgcgcacactgt-ttcttctgctctggaatt---atctttggctctgtttct-ttgcctaaa---ctttgt--ctgctgaacacag---
 ||||| || ||||| ||  | |||||||| ||||||||||| || || |||| | ||||||| |||||||| || ||||| ||||| | ||| || |||||      |        ||  |||  | |  |   | || |||   |  | |||| |  |  || |||  ||||   || | |  || ||  | ||     
TGCATTTAGGTGGATTACTCTCCTGCTGACTCAGGAATTCAATTTTGCTGACAGCCTTCACATTTGGGACACTCTTTTAAGCGATCCAGATGGTCCTCAGgtacaattcatattta-accttgtactataatcgacaggcatttaaacttatggcccactgcctcttgtataaaaaactatatagctacttgatactttta

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211178282|gb|FL421437.1|FL421437

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCAC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|28985507|gb|CB350674.1|CB350674

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|78076513|gb|DV504947.1|DV504947

EST:     ACACCATTCACATTTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|60342736|gb|DN209709.1|DN209709

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|148935026|gb|EC879067.2|EC879067

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|18164380|gb|BM334219.1|BM334219

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|71309792|gb|DR791178.1|DR791178

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|71309794|gb|DR791179.1|DR791179

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|18171551|gb|BM341391.1|BM341391

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

EST: gi|18170879|gb|BM340719.1|BM340719

EST:     ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAG                         GAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC
genomic: ACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 41 (downstream exon)
Score: 20

CGCATTCAGATGGATAACCTTACTGCTGACCCAGGAATTCAACTTCGCCGACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcg ... ctgaacacagGAAACCTTGCTCAGAATATGCTGCGCGATGCTGATCCTTGTCCGGAAGCGCCTCCTGGCCGGCGACTTCACCTCCAACCTCAAGCTCCTGCAGAATTACC

Block sizes: 47 (upstream exon), 42 (downstream exon)
Score: 14

Block sizes: 45 (upstream exon), 42 (downstream exon)
Score: 6

Block sizes: 45 (upstream exon), 25 (downstream exon)
Score: 13

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CGCATTCAGATGGATAACCTTACTGCTGACCCAGGAATTCAACTTCGCCGACACCATTCACATCTGGGACACACTATTAAGTGATCCTGGTGGCCCCCAGgtgagccgcgagcgcgcacactgtttcttctgctctggaattatctttggctctgtttctttgcctaaactttgtctgctgaacacag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TGGTGG