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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttttatttatctctatgtttgcttgtttatgtaagcagaggtccgttcaatttttctttacaatttggccattatcaattctccatcctga...

Basic information

species Zea mays
transcript GRMZM2G000645_T02
intron # 8
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: LOC_Os06g05180.2 (Oryza sativa), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttttatttatctctatgtttgcttgtttatgtaagcagaggtccgttcaatttttctttacaatttggccattatcaattctccatcctga-
|||| || ||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||| |||||||| |||||||||||||||||||||||||  | |       || |  | | | |||||||||| ||||   | |    |   |   |  || ||| | |||       | || |      |  |    
CACACAACATTTCTGCTGTTTGTTTCCATCCTGAGCTTCCTATAATCATTACTGGATCTGAAGATGGCACAGTTCGCATATGGCATTCGACAACTTACAGgttgctttcacagtttcacttgtgtttttgcttgttgatgtggactgtttcctagttc-tcattgtttgctattcaagtgctctctacgaaagaaacaact

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: GLYMA08G22140.1 (Glycine max), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaac---atgtt-tttatttatctctatgtttgcttgtttatgtaagcaga---ggtccgttcaatttttctttacaatttggc-cattatcaattctccatcctga
|||| ||| | ||||||||||| || |||||||| || ||||||||||||||||| ||||| ||||| ||||| || || |||||||| |||||||| ||||||    |||||  | |   ||   |  |||      ||||| |   || |     |  | |  |||  ||||  |  |||| | || | |||   |||        
CACACAATGTATCTGCTGTTTGCTTTCATCCTGAACTACCTATAATCATTACTGGCTCTGAGGATGGAACAGTGCGCATTTGGCATTCAACAACTTATAGgtaattttatgttgctcacagattattgagttgagagttttattttttcatatttaatttggtgcattgctcttgtctttttgtctcaatgtcatcactc--------

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: GLYMA07G03890.1 (Glycine max), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaac---atgtt-tttatttatctctatgtttgcttgtttatgtaagcaga---ggtccgttcaatttttctttacaatttggc-cattatcaattctccatcctga
|||| ||| | |||||||| || || |||||||| || ||||||||||||||||| ||||| ||||| ||||| || || |||||||| |||||||| ||||||    ||||| || |   ||  ||  |||      ||||| |   |  |     |  | |  |||  ||||  |  |||| | || | |||   |||        
CACACAATGTATCTGCTGTATGCTTTCATCCTGAACTACCTATAATCATTACTGGCTCTGAGGATGGCACAGTGCGCATTTGGCATTCAACAACTTATAGgtaattttatgttgttcacagattactgagttgagagctttattttttcttatctaatttggtgcattgctcttgcctttttgtctcaatgtcatcactc--------

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: GLYMA13G43680.2 (Glycine max), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttttatttatctctatgtttgcttgtttatgtaagcag-aggtccgttcaatttttctttacaatttggccattatcaattctccatcctga---
|||| ||| | |||||||| || || |||||||| || ||||||||||||||||| ||||| ||||| ||||| || |||||||| || |||||||| ||||||  ||     ||  |    | || |   || |    || | ||  | | |  ||| ||||  | |       | ||  || |     |  |||| | |   
CACACAATGTATCTGCTGTATGCTTTCATCCTGAACTTCCTATAATCATTACTGGTTCTGAGGATGGCACAGTACGCATATGGCACTCCACAACTTATAGgtaatgtga---aatgcacagat-tgctgagttatgagggttaacattatgcctattctatttggcatatgccgctagcttttcttctcccatcatcatcactc

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: GLYMA15G01680.1 (Glycine max), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttt-tatttatctctatgtttgcttgtttatgtaagcagaggtccgttcaatttttctttacaatttggccattatcaattctccatcctga---
|||| ||| | |||||||| || || |||||||| || ||||||||||||||||| ||||| ||||| ||||| || |||||||| || |||||||| ||||||  ||   |  |   ||  ||  |||     |||||    |    | |    ||| ||||  | |     | |  |  || |     |  |||| | |   
CACACAATGTATCTGCTGTATGCTTTCATCCTGAACTTCCTATAATCATTACTGGTTCTGAGGATGGCACAGTACGCATATGGCACTCCACAACTTATAGgtaatgtgaagtgcacagattactgagttatgaggttta----acattacgcttattctatttggcatatgcctctaccttttcttctcccatcatcatcactc

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: AT1G79990.1 (Arabidopsis thaliana), 5'ss of exon 12
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttttatttatctctatgtttgct--tgtttatgtaagcagagg-tccgttcaatttttctttacaatttggccattatcaatt--ctccat-cctga-------
|||| ||| ||||||| || | ||| ||||| ||||| |||||||| ||||| ||||| || ||||| || ||||| ||||||||  | || |||||||||||||  || |    |   |  |     ||||  || || |||    | |   | | |||||||||||   |   ||   |||  |    ||  ||  || | |||       
CACACAATGTTTCTGCAGTATCTTTTCATCCAGAGCTACCTATAATTATTACAGGATCCGAGGATGGAACTGTTCGCATATGGCACGCCACCACTTACAGgtaactcgtatgctgtctgcattggagctgctattgattttgtctttaaaaacttccttcaatttttcaaaaatcttcatccaacagttttttacttaatacttgacatctag

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: PP1S243_63V6.1 (Physcomitrella patens), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt-tttatttatct---ctatgtttgcttgtttatgtaagcagaggtccgttcaatttttctttacaatttggccattatcaattctccatcctga-
| |||||| | ||||| || |||||||||||||||||||| || ||  | ||||| || |||||||| || |||||||| |||||| | || || ||||||||  || || | ||||| |     | || |     |  | |  | |    | ||  || | ||   | || | |   |   ||| |||   | |  |   ||  
CTCATAATGTGTCTGCAGTGTGTTTCCATCCTGAGCTCCCCATCATTTTAACTGGTTCAGAAGATGGGACTGTTCGTATTTGGCATGCAACCACATACAGgtaggattttgtctatttttaaaaaccatttggaaatactaagatgattgaatgttagtgcgatgcat-ttaataccata--catgatcg--tgtttaagttggt

upper sequence: GRMZM2G000645_T02 (Zea mays), 5'ss of exon 8
lower sequence: PP1S79_180V6.1 (Physcomitrella patens), 5'ss of exon 8
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttttatttatctctatgtttgcttgtttatg---taagcagaggtccgttcaatttttctttacaatttggccattatcaattctccatcctga
| || ||| | ||||| |  ||||| ||||| ||||||||||| ||| ||||||| || |||||||| ||||||||||| |||||| | || || ||||||||     ||  | ||| |  || | ||   | | || ||   |  |  ||  | ||||   |||    ||  ||| |||    | |  || ||  |   || 
CTCACAATGTATCTGCAGCGTGTTTTCATCCCGAGCTCCCTATCATCCTTACTGGTTCAGAAGATGGAACAGTTCGTATTTGGCATGCAACTACGTACAGgtatacgtttcctctttcttcctctcttccatagcttttggattgtgtggactttcgtt-gttttcagatttgaatatgggttctctgtat-ctttaaaatg-

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|26457397|gb|CA828980.1|CA828980
EST:     ACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAG                         ACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATA
genomic: ACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt ... tattctgtagACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATA
EST: gi|32865242|gb|CF004924.1|CF004924
EST:     GAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAG                         ACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATA
genomic: GAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt ... tattctgtagACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATA

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 180

CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt ... tattctgtagACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATACATGAAGGGGTCTAGAAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 60

CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt ... tattctgtagACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATACATGAAGGGGTCTAGAAG


Block sizes: 45 (upstream exon), 49 (downstream exon)
Score: 64

CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt ... tattctgtagACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATACATGAAGGGGTCTAGAAG


Block sizes: 44 (upstream exon), 48 (downstream exon)
Score: 107

CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtt ... tattctgtagACTTGAGAACACACTAAACTATGGCCTTGAGAGAGTCTGGGCTGTTGGATACATGAAGGGGTCTAGAAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CACATAATATTTCTGCTGTTTGTTTCCATCCTGAGCTCCCTATAATCATTACTGGATCTGAAGATGGTACAGTTCGTATATGGCATTCGACAACTTACAGgtaacatgtttttatttatctctatgtttgcttgtttatgtaagcagaggtccgttcaatttttctttacaatttggccattatcaattctccatcctga

- - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGATG