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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctattactgatgatccttatttgttcctcctctcagcctgtatctgttttattttctgaactaaccttcatcgttcacatttgcag

Basic information

species Zea mays
transcript GRMZM2G049672_T01
intron # 7
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM2G049672_T01 (Zea mays), 5'ss of exon 7
lower sequence: LOC_Os02g55200.2 (Oryza sativa), 5'ss of exon 7
TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctattactgatgatccttatttgttcctcctctcagcctgtatctgttttattttctgaactaaccttcatcgt--------tcacatttgcag
|||||| |||||||| |  |||||||||||||||||||||||||||  |||||||||||| ||||||||||| |||| | |  | |  || || ||||||||||||| |||| ||  |  |    ||    || ||   ||     | | | |  |  |||  ||| || || ||| |          || |||||||
TCTTGGGAACGAAAGCATGGCAGTTGAGGGTTTTGAGGAAGCAATTAAATGCCTTGAGAATTTGAAGCTGGAATCTGGAGAGGCCAGTCTTGAGCAGCGGgtagctactacttatccttgtgtactgcctttcatccatgcgctcctatttgtggtgctcttgactgacttttatcatgtactgtgctacttttgcag

upper sequence: GRMZM2G049672_T01 (Zea mays), 5'ss of exon 7
lower sequence: LOC_Os01g01420.1 (Oryza sativa), 5'ss of exon 7
TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctattactgatgatccttatttgttcctcctc--tcagcctgtatctgttttattttctg-aactaaccttcatcgttcacatttgcag--------
|||||| |||||||| |  |||||||||||||||||||||||||||  |||||||||||| ||||||||||| |||| | |  | |  || || ||||||||||||| |||| ||  |  |    ||    || ||  |  ||   ||| |||  |  | ||    ||| || || ||| |  ||  |   |         
TCTTGGGAACGAAAGCATGGCAGTTGAGGGTTTTGAGGAAGCAATTAAATGCCTTGAGAATTTGAAGCTGGAATCTGGAGAGGCCAGTCTTGAGCAGCGGgtagctactacttatccttgtgtactgcctttcatccatgcgctcctatttgtggtgctctcgacgactgacttttatcatgtactgtaatacttttgcag
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|60340202|gb|DN207175.1|DN207175
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|12972826|gb|BG268175.1|BG268175
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|18171342|gb|BM341182.1|BM341182
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|149087898|gb|EE287177.2|EE287177
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|211393982|gb|FL446401.1|FL446401
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|78179829|gb|DV550202.1|DV550202
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|78025114|gb|DV493501.1|DV493501
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|18179814|gb|BM381024.1|BM381024
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|14245501|gb|BG874083.1|BG874083
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|13516136|gb|BG518412.1|BG518412
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|67021056|gb|CO449805.1|CO449805
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|5361197|gb|AI795617.1|AI795617
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|148999090|gb|EE028420.2|EE028420
EST:     GCCTTGAGAAGTTGAAGCTGGATTTTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTTTTGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|5706152|gb|AI941928.1|AI941928
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCCCAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|32863820|gb|CF003502.1|CF003502
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|148958312|gb|EC904534.2|EC904534
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|149051662|gb|EE155775.2|EE155775
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|13150752|gb|BG321074.1|BG321074
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|18655055|gb|BM499860.1|BM499860
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|33100566|gb|CF060526.1|CF060526
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|22490004|gb|BU049927.1|BU049927
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|211234748|gb|FL433592.1|FL433592
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|6022117|gb|AW067045.1|AW067045
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|32909333|gb|CF014145.1|CF014145
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|14244109|gb|BG841797.2|BG841797
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|16926910|gb|BM079978.1|BM079978
EST:     AAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: AAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|83278529|gb|DV942537.1|DV942537
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|32909332|gb|CF014144.1|CF014144
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|6859948|gb|AW355942.1|AW355942
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTAGCCTCGAACAACGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|16925308|gb|BM078376.1|BM078376
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|13515841|gb|BG518117.1|BG518117
EST:     AAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: AAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
EST: gi|4967168|gb|AI692078.1|AI692078
EST:     GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGG                         CGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC
genomic: GCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 186

TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGACATAGTCTTCTCCTAAGAAGGCACCACCAGCAGATATCTCCTCATGGTAA


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 159

TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGACATAGTCTTCTCCTAAGAAGGCACCACCAGCAGATATCTCCTCATGGTAA


Block sizes: 48 (upstream exon), 47 (downstream exon)
Score: 150

TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGACATAGTCTTCTCCTAAGAAGGCACCACCAGCAGATATCTCCTCATGGTAA


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 226

TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctatta ... acatttgcagCGCCTCTCGGTTCTCGACTTCCTGCACAACCAGTTGGCCGATAAGTAAGACATAGTCTTCTCCTAAGAAGGCACCACCAGCAGATATCTCCTCATGGTAA


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
TCTTGGTAACGAAAGTACCGCAGTTGAGGGTTTTGAGGAAGCAATTCGATGCCTTGAGAAGTTGAAGCTGGATTCTGAACAAACTACCCTCGAACAGCGGgtagctattactgatgatccttatttgttcctcctctcagcctgtatctgttttattttctgaactaaccttcatcgttcacatttgcag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - AGAAGT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  TTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGC
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGCTG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGGA