ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

GGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagttattgcttgattctgcatatcaattattagctaatttggtagctgcagcttcactattattgctccaaaaggccagtgttgaccatcttcctttctttttgtaacag

Basic information

species	Zea mays
transcript	GRMZM2G366935_T05
intron #	6
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G366935_T07 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os01g40840.2 (Oryza sativa), 5'ss of exon 5

GGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagttattgcttgattctgcatatcaattattagctaatttggtagctgcagcttca--ctattattgctccaaaaggccagtgttgaccatctt--cctttctttttgtaacag---
|||||||||||||||||||||||||||||||||||||||| ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||| |    ||| ||||||||||||||||||| ||| | |||| ||||||||| | ||||  ||  | |||||  ||| |  ||   |||||||||||  ||||||||| |   | |    
GGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCAACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgtga----ttgtttgattctgcatatcaattcttaacaaattcggtagctgctgattcattcttctgttgctgaaaaggagcatgcttgaccatcttttcctttctttcttctaaaatag

upper sequence: GRMZM2G366935_T07 (Zea mays), 5'ss of exon 5
lower sequence: GLYMA06G08480.2 (Glycine max), 5'ss of exon 5

GGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagttattgcttga-ttctgcatatcaattattagctaatttggtagctgcagcttcactattattgctccaaaaggccagtgttgaccatcttcctttctttttgta-acag---------------------------------------------------------
|| |||||||| | |||||||||||||||||||| |||| | || ||||||||||| ||||| ||||| |||||||| || || |||||||||||||||||| |||   |||||  | |  ||| |  |||   | ||     | | |     ||   |||| || |  |||   |||           ||| ||  ||| |||| |    | |  |||                                                         
GGACCAAGCTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTCCCTGTGGATCTTGTTCGGGAATTTGGACGTCAGCTTTTGGAATCTGTAGCATgtctgtggattattcttggccttcagtttatttctcatgtttcattcttaagtctcttgctt-acaa--attaaaccatggctttttccttggcctgctttctttgtccagggatgcagtgtatcttggttgtgtctgcttactttaacttctttcttgttctttatcatattcag

upper sequence: GRMZM2G366935_T07 (Zea mays), 5'ss of exon 5
lower sequence: Vv04s0008g04220.t01 (Vitis vinifera), 5'ss of exon 6

GGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagttattgcttgattctgcat-atcaattattagctaatttggtagctg---cagct--tcactattattgctccaaaaggccagtgttgaccatcttc--ctttctttttgtaacag------------------------------------------------------
|| ||||| || | |||||||||||||||||||| |||| | || || |||||||| ||||| ||||| |||||||| || || ||||||||||||||||||||||   || |   | |    ||| | |||  | |||   || |||  |  ||     | |   | ||    ||   |      |||| || |||    ||     || |   | ||||  |                                                       
GGACCAAGTTTATTTGATTTTCTAAAGAGAAATAAATACTGCCCATTTCCTGTGGATCTTGTTCGGGAATTTGGACGTCAGCTTTTGGAATCTGTAGCATgtgtgtgcattgtccttggcccttgctttatctctcattctgtattttattttctaacctaaccaattacccagatctgttttttgggcctgttttgtttgtccaggactgtacctctgtattatgtttgtgtcctttttgttttaacatgattttccttgctttacaatatccaacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|21331443|gb|BQ486824.1|BQ486824

EST:     TGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCAT                         ATATGCATGACTTGAGGCTTATCCACACTGATCTGAAGCCAGAAAACATAC
genomic: TGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagt ... tttgtaacagATATGCATGACTTGAGGCTTATCCACACTGATCTGAAGCCAGAAAACATAC

EST: gi|148930529|gb|EE285130.2|EE285130

EST:     TGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCAT                         ATATGCATGACTTGAGGCTTATCCACACTGATCTGAAGCCAGAAAACATAC
genomic: TGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagt ... tttgtaacagATATGCATGACTTGAGGCTTATCCACACTGATCTGAAGCCAGAAAACATAC

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 140

GGGCCAAGCTTGTATGATTTTCTAAAGAGAAATAGATACCGACCTTTCCCTGTGGAACTTGTGCGGGAGTTTGGACGGCAACTGTTGGAATCTGTAGCATgtgtgttagt ... tttgtaacagATATGCATGACTTGAGGCTTATCCACACTGATCTGAAGCCAGAAAACATACTACTTGTGTCTTCTGAGTATATAAAAGTTCCAAGTACAAAG

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 109

Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 152