ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TCACACAAACCTACGTAGAGTGACATACCTTGTGTTGGATGAGGCGGACCGGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttcgtttagaaatgcagaattcttactaatatctattgtcttgcctagctctaatgcttgtccaacacttctgctatccttag

Basic information

species	Zea mays
transcript	GRMZM2G362850_T03
intron #	6
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G362850_T01 (Zea mays), 5'ss of exon 6
lower sequence: LOC_Os01g68320.3 (Oryza sativa), 5'ss of exon 5

TCACACAAACCTACGTAGAGTGACATACCTTGTGTTGGATGAGGCGGACCGGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttcgtttagaaatgcagaattcttactaatatct--attgtcttgcctagctctaatgcttgtccaacacttctgctatccttag
|||||| ||||| || ||||| |||||||| ||  |||||||||| ||||| ||||| || ||||| ||||||||||| ||||| |||||||| ||||||| |  | ||  |||||||       ||   | ||  | | |    |  ||  ||    | |      ||   |||  || || | |   |||
TCACACTAACCTGCGAAGAGTAACATACCTCGTCCTGGATGAGGCTGACCGCATGCTTGACATGGGTTTTGAGCCCCAGATTCGTAAAATAGTGGCACAGgcatggcttgttttagaatgcttatatcagttctggtgttttggctaactctccattttttctattgtgctaaacgtttttgttgttaatag

upper sequence: GRMZM2G362850_T01 (Zea mays), 5'ss of exon 6
lower sequence: GLYMA05G02590.1 (Glycine max), 5'ss of exon 5

TCACACAAACCTACGTAGAGTGACATACCTTGTGTTGGATGAGGCGGACCGGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttcgtttagaaatgcagaattcttactaatatctattgtcttgcctagctctaatgcttgtccaacacttctgctatccttag-------------
 |||||||||||    |||||||| |||||||| |||||||| || || || |||||||| ||||| ||||| || || || |||||||| ||||| |||||||  ||| |     ||      |    ||| || |   || ||        |    |      | |    || || ||  | |||||              
GCACACAAACCTGAAGAGAGTGACTTACCTTGTCTTGGATGAAGCTGATCGAATGCTAGACATGGGTTTTGAACCTCAGATACGGAAAATTGTAGCCCAGgtacttttttgcaactaacctgtaacaagttagtactgagtagtggggaaaagaaacattgaatctatttc-catttttg--aaccttaagtgtgctgttgtc

upper sequence: GRMZM2G362850_T01 (Zea mays), 5'ss of exon 6
lower sequence: EDP09760 (Chlamydomonas reinhardtii), 5'ss of exon 4

TCACACAAACCTACGTAGAGTGACATACCTTGTGTTGGATGAGGCGGACCGGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttcgtttagaaa----tgcagaattcttactaatatc---tattgtcttgcctagctctaatgcttgtccaa-cacttctgctatccttag--
   ||| ||||| ||  | ||||||||||| ||| |||| ||||||||||| ||||| || ||||| |||||||| || || || || || || |  |||||   |   ||    | |     || |     |     ||        |  |    ||   ||   ||  |  | ||   |||  | |  |  | ||   
CATCACCAACCTGCGGCGTGTGACATACCTGGTGCTGGACGAGGCGGACCGCATGCTGGACATGGGCTTTGAGCCGCAGATCCGCAAGATTGTGGACCAGgtggggcggcggggcggaggcggtgtatggagcggggcaaggcggggcagggcagggcagggcagcaagccggggccgggcacgacgggcagtcctacag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|31354193|gb|CD438550.1|CD438550

EST:     GGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAG                         ATCCGCCCACACAGGCAAACCTTGTACTGGAGTG
genomic: GGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttc ... ctatccttagATCCGCCCAGACAGGCAAACCTTGTACTGGAGTG

EST: gi|88752664|gb|DY536805.1|DY536805

EST:     GGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAG                         ATCCGCCCAGACAGGCAAACCTTGTACTGGAGTGCCACATGGCCTAGAGAA
genomic: GGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttc ... ctatccttagATCCGCCCAGACAGGCAAACCTTGTACTGGAGTGCCACATGGCCTAGAGAA

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 465

TCACACAAACCTACGTAGAGTGACATACCTTGTGTTGGATGAGGCGGACCGGATGCTAGATATGGGGTTTGAGCCCCAAATTCGGAAAATAGTAGCACAGgtacagtttc ... ctatccttagATCCGCCCAGACAGGCAAACCTTGTACTGGAGTGCCACATGGCCTAGAGAAGTTGAAGCCTTAGCAAGACAGTTTCTACAAAATCCTTACAAG

Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 464

Block sizes: 48 (upstream exon), 48 (downstream exon)
Score: 451