ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq
cis-regulatory elements

Sequence

atgc intronic sequence ATGC exonic sequence

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatctagtacccagtatcattactacacccttttattatctgatgttttagattttaagtacacttaccgtgctgcctttttaaaattacatccc...

Basic information

species	Zea mays
transcript	GRMZM2G092129_T02
intron #	6
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G092129_T02 (Zea mays), 5'ss of exon 6
lower sequence: LOC_Os07g22640.4 (Oryza sativa), 5'ss of exon 6

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatctagtacccagtatcattactacacccttttattatctgatgttttagatttta-agtacacttaccgtgc-tgcctttttaaaattacatccc-
||||| |||||||| ||||| ||  |  | |||||| |||||||||| || || || ||||||||||| |||||||||||||| ||||| ||||| || | |   |||||  |   |  || |||  |    |  |||   |   ||| ||||   |   | | ||||       ||| || ||    | |      ||   
CTCAAAGATCTTCAAGTCTACACCATTGTCCGTGTTGTATTTCAACTATCAGAGGAGATCCCTTGCATCTCTGCTGTTGTTGTTGCTCTTCTTGCAGAGgtatacatctacaa---aaaatgatttatctttctatttcaaaggagattttttttctaagacaatacaggagatctgcatgtctaagcagagacgagtcttg

upper sequence: GRMZM2G092129_T02 (Zea mays), 5'ss of exon 6
lower sequence: GLYMA03G02370.1 (Glycine max), 5'ss of exon 6

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatctagtacccagtatcattactacacccttttattatctgatgttttagattttaagtacacttaccgtgctgcctttttaaaattacatccc-
 | |||||||| ||||| |  ||  |  | ||||| |||||||||||  ||||||| ||||| |||||||||||||||||||| ||||| |||||||| | ||   |   |      |       | ||||     ||  ||| ||| |   |  | ||    || | ||   || ||  | ||    |   | |     
TTGAAGGATCTACAGGTTTTCACCATTGTCCGTGTGATATTTCAACTTGCTGAAGAGATCCCCTGCATTTCTGCTGTTGTTGTAGCTCTGCTTGCTGAGgtaatccttgtgaggagggagaggagaatacaatgagttcatatatgaaggaatgtaatt-gcatatagttcttgttctttccttacccatcaataatatt

upper sequence: GRMZM2G092129_T02 (Zea mays), 5'ss of exon 6
lower sequence: GLYMA18G45720.1 (Glycine max), 5'ss of exon 6

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatctagtacccagtatcattactacacccttttattatctgatgttttagattttaagtacacttaccgtgctgcctttttaaaattacatccc-
 | |||||||| ||||| |  ||  | || ||||||||||| |||||  |||| || || || |||||||||||||||||||| || || ||||| || | || | |  || |   |  |                         |||| |   |   |||||| |   |      |   ||         | |   || 
TTGAAGGATCTCCAGGTTTTCACCATTATCCGTGTTATATTCCAACTTGCTGACGAGATTCCCTGCATTTCTGCTGTTGTTGTTGCCCTACTTGCCGAGgtaacttttgag-atggaaaaatgaaggaggggagggggggggggggatgatgatgcacttaagttccagtgtttcacaatctcccattttgtgctctcct

upper sequence: GRMZM2G092129_T02 (Zea mays), 5'ss of exon 6
lower sequence: GLYMA07G09070.1 (Glycine max), 5'ss of exon 7

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtat--ctagtacccagta--tcattactacacccttttattatctgatgttt-tagattttaagtacacttaccgtgctgcctttttaaaattacatccc
 | |||||||| ||||| |  ||  |  | ||||| ||||| |||||  ||||||| |||||||||||||||||||||||||| ||||| |||||||| | ||   |  ||     |  |||  |   ||| | |   | |  |      || ||| | | ||||  || |        |  |||  ||   || ||       
TTGAAGGATCTACAGGTTTTCACCATTGTCCGTGTGATATTCCAACTTGCTGAAGAGATCCCTTGCATTTCTGCTGTTGTTGTAGCTCTGCTTGCTGAGgtaatccttgctgaaggcatgtaaatgcatacgaaaggatatagtggctctatctttattggttttttgttcctcctttttattgctattccgaagtttt-----

upper sequence: GRMZM2G092129_T02 (Zea mays), 5'ss of exon 6
lower sequence: AT3G61050.1 (Arabidopsis thaliana), 5'ss of exon 7

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatctagtacccagtatcattactacacccttttattatctgatgttttagattttaagtacacttaccgtg-ctgcctttttaaa--attacatccc-
 | ||||||||||| || |  || ||   ||||||||| ||||||||  | || || || ||||| || |||||||||||||| ||||| |||||||| | |    | |  |    ||   ||  |  | |  | ||| |  | |    ||||| || |   ||   |||||  || |   || ||||||  ||  || ||  
TTGAAGGATCTTCAAGTTTTCACAGTTGCACGTGTTATCTTTCAACTTGCGGATGAGATTCCTTGTATATCTGCTGTTGTTGTTGCTCTACTTGCTGAGgtacacttttcctgtaaaggtccagaataagattcatttttcttatatcattttatatctcctgt---cttacaatgaccaactatttaaatgatctcaaccag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|211220819|gb|FL457378.1|FL457378

EST:     GCTGTTGTTGTGGCTCTCCTTGCTGAT                         CCAGAGCCAAAAATTCAGTACACCCTSAAGGCCATTGGAGSAAGCCTAACT
genomic: GCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT

EST: gi|211335355|gb|FL444766.1|FL444766

EST:     CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGAT                         CCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT
genomic: CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT

EST: gi|148971559|gb|EE022272.2|EE022272

EST:     TGAAGACATCCCTTGCATTTCTGCTGGGTGT-GTGGCTCTCCTTGCTGAT                         CCAGAGCCAAAAAATCAGTACACCCTGAAGGCCA
genomic: TGAAGACATCCCTTGCATTTCTGCTGT-TGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCA

EST: gi|211220820|gb|FL457379.1|FL457379

EST:     CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGAT                         CCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT
genomic: CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT

EST: gi|71331353|gb|DR802511.1|DR802511

EST:     CTGAAGACATCCCTTGCATTTCTGCTGTTGGTGTGGCTCTCCTTGCTGAT                         CCAGAGCCAAAAATTCAGTACACCCTGGAGGTCATTGGAGGAAAGC-TAAC
genomic: CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAA-GCCTAAC

EST: gi|111145863|gb|EE258112.1|EE258112

EST:     CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGAT                         CCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT
genomic: CTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACT

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 47 (upstream exon), 49 (downstream exon)
Score: 158

CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatct ... tgtgctccagCCAGAGCCAAAAATTCAGTACACCCTGAAGGCCATTGGAGGAAGCCTAACTGCAGTTCCTGGACTTTCTGACATGATTGAT

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 233

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 160

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 191

Putative cis-regulatory sequences

atgc	intron	ATGC	exonic elements by Pertea et al.
ATGC	exon	atgc	putative intronic elements
		ATGC	putative exonic elements identified for retained introns

        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
CTCAAGGATCTTCAGGTCTATACTGTCATACGTGTTATATTTCAACTGTCTGAAGACATCCCTTGCATTTCTGCTGTTGTTGTGGCTCTCCTTGCTGATgcaagtatctagtacccagtatcattactacacccttttattatctgatgttttagattttaagtacacttaccgtgctgcctttttaaaattacatccc

- - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - GCTGTT
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GCTGAT