ERISdb: a Database of Plant Splice Sites

General
Orthologs
EST
RNA-Seq

Sequence

atgc intronic sequence ATGC exonic sequence

TTTATTCCAGCTTTCATTACCTCTGAACTTCCTTGGGAGTGTGTATCGAGAATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAGgtaaagttttatattgaaattatcaagtctttaaagaccttaatttcagtttcatactcaactcatgctctctgatactttagattatgttttaattttttaag

Basic information

species	Zea mays
transcript	GRMZM2G032936_T01
intron #	6
splice site	5'
intron type	U2

Orthologous splice sites

atgc intronic sequence ATGC exonic sequence

upper sequence: GRMZM2G032936_T01 (Zea mays), 5'ss of exon 6
lower sequence: LOC_Os06g03770.1 (Oryza sativa), 5'ss of exon 11

TTTATTCCAGCTTTCATTACCTCTGAACTTCCTTGGGAGTGTGTATCGAGAATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAGgtaaag-ttttatattgaaattatcaagtctttaaagacct-taatttcagtttcatactcaactcatgctctctgatacttta--gattatgttttaattttttaag
| ||||||| ||||| || ||||||||||||||||| ||||| |||||||||||||| |||||||| |||||||||||||| |||||||| ||||||||||||||   ||  |    |  |  |   | | | || | | |  | |||||||||||| ||    |||    | | || |||| |  | |  | ||| | ||| || ||
TCTATTCCAACTTTCTTTGCCTCTGAACTTCCTTGGAAGTGTCTATCGAGAATCTAGGCAAAGCCTGATTGACATGAAGTCTATGTTTCAATTGCTTGAGgtaaaaacttcgtgactatctgttgggggcctaaatggcttgcagtttcagtttcatcctt---tcacaaccccagaaacttcattgctgttatttcactttgttcag

upper sequence: GRMZM2G032936_T01 (Zea mays), 5'ss of exon 6
lower sequence: GLYMA14G38800.1 (Glycine max), 5'ss of exon 10

TTTATTCCAGCTTTCATTACCTCTGAACTTCCTTGGGAGTGTGTATCGAGAATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAGgtaaagttttatattgaaatt--atcaagt-ctttaaagaccttaatttcagtttcatactcaactcatgctctctgatactttagattatgttttaattttttaag---
| | ||||| || ||  | || || || |||||||| ||||| ||||| ||  |||  || || ||  ||||||||||||| ||||||||||| |||||||||   |      | ||| ||  || || | | ||  ||   ||||||| |  |   || |    |  |    | ||| | ||     | | ||||| ||   ||||   
TCTCTTCCAACTATCTCTTCCACTCAATTTCCTTGGTAGTGTTTATCGTGAGACTATTCAGAGTCTAGTTGACATGAAGTCCATGTTTCAGTTACTTGAGgtatcatccc-cgtggaattttaatgaattaccttgcag---ttaattttaaatatgtatt--gtttgttgattatgaaattt----ctttcttttacttcagtaagtac

upper sequence: GRMZM2G032936_T01 (Zea mays), 5'ss of exon 6
lower sequence: Vv06s0004g08350.t01 (Vitis vinifera), 5'ss of exon 13

TTTATTCCAGCTTTCATTACCTCTGAACTTCCTTGGGAGTGTGTATCGAGAATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAGgtaaagttttatattgaaattatcaagtctttaaagaccttaatttcagtttcatactcaactcatgc--tctctgatactttagattatgttttaattttttaag
| |||| ||||| ||  | || || ||||| || || ||||||||||| ||| ||| |||||| ||  |||||||||| || |||||||| || |||||||||    || |     | ||  || ||| |||   | |   | ||| || |||||      |   | |  | |||| || |||    | |  | || ||| ||   
TCTATTTCAGCTATCTCTTCCACTCAACTTTCTCGGCAGTGTGTATCGTGAAACTATACAAAGTCTTGTTGACATGAAATCCATGTTTCAATTACTTGAGgtattacttaag--caatatcttctagtttttcctggc---agtttaagattcattgatgtcaagttcaatttctgttagttttatctgttatatagtttatttt-

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments

ATGC    EST sequence
ATGC    genomic sequence (exon)
ATGC    genomic sequence (truncated intron)

EST: gi|160481626|gb|EY254458.1|EY254458

EST:     AATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAG                         GAAAAACCTGCAATAAAGGATGAGCCTCATGCACAACCTTTGCAGTTCAAG
genomic: AATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAGgtaaagtttt ... attttttaagGAAAAACCTGCAATAAAGGATGAGCCTCATGCACAACCTTTGCAGTTCAAG

RNA-Seq data

ATGC Supported by RNA-Seq exonic block that spans over the splice site

atgs Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.

Found data for 4 RNA-Seq libraries.

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 82

TTTATTCCAGCTTTCATTACCTCTGAACTTCCTTGGGAGTGTGTATCGAGAATCTAGACAAAGCCTCATTGACATGAAGTCAATGTTTCAGTTGCTTGAGgtaaagtttt ... attttttaagGAAAAACCTGCAATAAAGGATGAGCCTCATGCACAACCTTTGCAGTTCAAGGGAGGGTGCATTGAATTCGAGAATGTTCATTTTGG

Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 117

Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 108