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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtgattatatagggttgagatgaaatttctttttgagtagtcttggaacatattctagcagtttcttcaactttattagagattgctctttttcacccctaaatctgcaatataccccag

Basic information

species Zea mays
transcript AC209858.4_FGT002
intron # 6
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: AC209858.4_FGT002 (Zea mays), 5'ss of exon 6
lower sequence: LOC_Os03g05720.1 (Oryza sativa), 5'ss of exon 6
GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtgattatatagggttgagatgaaatttctttttgagtagtcttggaacatattctagcagtttcttcaactttattagagattgctctttttcacccctaaatctgcaatataccccag
||| ||  |  |||||||||| ||||||||| ||||||||||||| ||||| || || |||||||||||||||||||||||||||||||| |||||||||||||   |||||| ||| |  || |   |||   | || |||| |    |||  |  | | ||   | |||  | | || |    || |           |||    | |  ||||           
GTGTCTTGCCATCAGCAGTCGTGGTGATTTTATTGTAACAGGTTCTCATGACCGCTCTATTCGGCGTTGGGATCGTACTGAAGAACAACTCTTCATTGAGgtatgcagtgattgtattgttttaatg-gaatcctgttattgaatg--tttgctatctttgcttagatttttcttagctct----gaaacc---------cacaaaaataaatgcag----------

upper sequence: AC209858.4_FGT002 (Zea mays), 5'ss of exon 6
lower sequence: GLYMA03G34360.1 (Glycine max), 5'ss of exon 7
GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtgattatatagggttgagatgaaatttctttttgagtagtcttggaacatattctagcagtttcttcaactttattagagattgctctttttcacccctaaatctgcaat---ataccccag-----------
||| ||  || |||| | ||| ||||||||| | || || || || ||||||||||| ||||| || |||||||| |||||||| ||  | |||||||||||||       || |||  | |||     | | ||  |   |   |   |   |  | |   |   |   |    |  |||  ||| |  |  |  ||         || | || |||   |||  |||            
GTGTCTTGCAGTCAGTAATCGTGGTGATTTTATCGTCACTGGATCTCATGATCGTTCTATTCGCCGCTGGGATCGCACTGAAGAGCAGTTCTTCATTGAGgtatacacaacttgtatgagattgg--ctacacttaatgcaggacaaagtctaattacaaatttataacattagtacttttcatagggtgtttttcttcctggattaaattttggaatgagatagaccatttcatttgcag

upper sequence: AC209858.4_FGT002 (Zea mays), 5'ss of exon 6
lower sequence: Vv08s0007g04690.t01 (Vitis vinifera), 5'ss of exon 6
GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtgattatatagggttgagatgaaatttctttttgagtagtcttggaacatattctagcagtttcttcaactttattagagattgctc--tttttcacccctaaatctgcaatataccccag---
||| ||  || |||||| ||| ||||||||| ||||  | || || ||||| || |||||||  |||||||||||||||||||| |   | || ||||||||||    | | | | |    ||     | ||||      ||  | ||  ||| |||     |     |||  ||    | ||| | |  ||     | ||| |||     |  ||| || |  |   |   
GTGTCTGTCAATCAGCAATCGTGGTGATTTTATTGTCTCGGGATCTCATGACCGATCCATTCACCGTTGGGATCGTACTGAAGAGCCCTTTTTTATTGAGgtatggaattagtttgtgacattatatgggaattgaaacatggat-gttatggcacactaaattttttttttgtccctgtgattggggtgtgactgatgtttaaccaaaggaaatgctatctgacttcgcag

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|32835694|gb|CD975372.1|CD975372
EST:     ACTGAAGAACAACTGTTCATTGAG                         GAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
genomic: ACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
EST: gi|32835920|gb|CD975598.1|CD975598
EST:     TACTGAAGAACAACTGTTCATTGAG                         GAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
genomic: TACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
EST: gi|211417128|gb|FL448753.1|FL448753
EST:     ATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAG                         GAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
genomic: ATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
EST: gi|148933242|gb|EC876892.2|EC876892
EST:     ATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAG                         GAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
genomic: ATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
EST: gi|32833351|gb|CD973029.1|CD973029
EST:     ACTGAAGANCANCTGTTCATTGAG                         GAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
genomic: ACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
EST: gi|78084367|gb|DV512760.1|DV512760
EST:     ATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAG                         TAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC
genomic: ATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAAC

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 206

GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAACGATAATGACTATAGATATGGGCAGAAAGATGATGCCCCTGATGAAGGTT


Block sizes: 49 (upstream exon), 46 (downstream exon)
Score: 103

GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAACGATAATGACTATAGATATGGGCAGAAAGATGATGCCCCTGATGAAGGTT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 147

GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAACGATAATGACTATAGATATGGGCAGAAAGATGATGCCCCTGATGAAGGTT


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 137

GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtg ... tataccccagGAAGAGAAGGAGAAAAGATTGGAAGAAACCTTTGAGGCTGATTTAGAAAACGATAATGACTATAGATATGGGCAGAAAGATGATGCCCCTGATGAAGGTT


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GTGCCTCACACTCAGCAGTCGCGGTGATTTTGTTGTAACAGGTTCACATGATCGTTCCATTCGGCGTTGGGATCGTACTGAAGAACAACTGTTCATTGAGgtattttgtgattatatagggttgagatgaaatttctttttgagtagtcttggaacatattctagcagtttcttcaactttattagagattgctctttttcacccctaaatctgcaatataccccag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  CTGAAG
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - TGAAGA
- - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - AAGAAC