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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtctttgtatacctcctggatgatgtatttttaaacagtaagtggcaacacacttgtgtctcagaattgaaaatggtccatggtaaggtagatt...

Basic information

species Zea mays
transcript GRMZM5G880300_T02
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G880300_T02 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os01g07950.1 (Oryza sativa), 5'ss of exon 5
GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtg---tctttgtatacctcctggatgatgtatttt-taaacagtaagtggcaacacacttgtgtctcagaat-tgaaaatggtccatggtaaggtagatt
  |||||| || ||| |||||| ||| | || || || ||||||||||||||||| ||||||||||||||||||   ||||   || ||| |   ||  || |||   |      ||  ||  |    |||| ||| |   ||  ||  || |  | |   |  |       
ATGTCCCTATCAGTGCTAGAGATATTTTGGGAGACCTCAAGCTCAAAGAGTGTGTCAAAGCCCACACgtaagtggcttcttctcatgccttcctaatcttgcattaagtttcttttagatgatagacaacttatgtttttcaacatgggaaggaactagactgggag-----

upper sequence: GRMZM5G880300_T02 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os01g34620.5 (Oryza sativa), 5'ss of exon 5
GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtctttgtatacctcctggatgatgtatttttaaacagtaagtggcaacacacttgtgtctcagaattgaaaatggtcca--tggtaaggtagatt----
  |||||| || ||| ||| || ||| | || || || |||||||||||| |||| |||||||||||||||||| ||     |   ||||  || || |  | |||   ||  || |       |||||| | |||  |  | ||          ||||| | || |||    
ATGTCCCTATCAGTGCTAGGGATATTTTGGGAGACCTCAAGCTCAAAGAGAGTGTCAAAGCCCACACgtaagtggctcctggtcattccttcataatcttgtgttatgtagatagag------tacttgtttttcaacaggggaacaaactagactggtaggttaaattattc
















Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|74236784|gb|DT644698.1|DT644698
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|211479938|gb|FL425914.1|FL425914
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|91877551|gb|EB407508.1|EB407508
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|93017644|gb|EB643164.1|EB643164
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCANAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|76281315|gb|DV020883.1|DV020883
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|211328326|gb|FL186217.1|FL186217
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|84980275|gb|DW530624.1|DW530624
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|211069174|gb|FL417736.1|FL417736
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|101389214|gb|EB816562.1|EB816562
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCANAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|93017643|gb|EB643163.1|EB643163
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|74236783|gb|DT644697.1|DT644697
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
EST: gi|84980198|gb|DW530547.1|DW530547
EST:     AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACAC                         TAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG
genomic: AGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGG

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 46 (upstream exon), 49 (downstream exon)
Score: 52

GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGGGTCTGACATCATATTAAGCTTGCACCAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 70

GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGGGTCTGACATCATATTAAGCTTGCACCAG


Block sizes: 48 (upstream exon), 49 (downstream exon)
Score: 59

GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGGGTCTGACATCATATTAAGCTTGCACCAG


Block sizes: 49 (upstream exon), 49 (downstream exon)
Score: 58

GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtct ... gttggtgcagTAACTGGCCTACATTTCCACAAATATTTATCAAAGGAGAGTTTGTTGGGGGGTCTGACATCATATTAAGCTTGCACCAG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
GCGTCCCTGTCTGTGGTAGAGACATTCTTGGGGATCTTAAGCTCAAAGAGTGTGTTAAAGCCCACACgtaagtgtctttgtatacctcctggatgatgtatttttaaacagtaagtggcaacacacttgtgtctcagaattgaaaatggtccatggtaaggtagatt

- - - - - - - - - - - - - - - - - - -  AGCTCA