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Data associated with selected splice site

Sequence

 atgc   intronic sequence     ATGC   exonic sequence

ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccccccttttctggagtcttttttaagcacatcccaagagttagagaattgcatgacgaatggaacttgtttgtttggtgtgatccag

Basic information

species Zea mays
transcript GRMZM5G869572_T01
intron # 5
splice site 5'
intron type U2

Orthologous splice sites


 atgc   intronic sequence     ATGC   exonic sequence


upper sequence: GRMZM5G869572_T01 (Zea mays), 5'ss of exon 5
lower sequence: LOC_Os04g59494.1 (Oryza sativa), 5'ss of exon 25
ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccccccttttctggagtcttttttaagcacatcccaagagttagagaattgcatgacgaatggaacttgtttgtttggtgtgatccag
| ||||| |||||||||||||||||| ||||||| ||||||||||| |||||||| || ||||| |||||||||||||| |||||||| || ||||||||||||| | |   | |||    |  || | |   || | |     ||  ||   | || | | | | |  |                         
ATAGCGCAATCACTCTTGGGAGGCTTTGCTGGGTCTGTCCAGACATTGTGGCCCCCCACATGGACCATTTCATGCAAGCCTGGTGCAATGCCTTGTGCATgtaagtgtctgtccttt----aagctctctggtgcccttttatggatcta---attttcgtcatgtacag-------------------------

upper sequence: GRMZM5G869572_T01 (Zea mays), 5'ss of exon 5
lower sequence: Vv03s0038g02830.t01 (Vitis vinifera), 5'ss of exon 25
ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccccccttttctggagtcttttttaagcacatcccaagagttagaga-attgcatgacgaatggaacttgtttgtttggtgtgatccag----
| || || ||||| ||||||||||||   ||||| |||||||| || ||| | |  |||||||||||||| |||||| ||||||| |  || || | |||||| |      | |  ||      |     |      || |      ||       ||     || |    |  |   ||| |   | |  |   |    
ATAGTGCCATCACCCTTGGGAGGCTTGCTTGGGTCTGTCCAGAAATTGTGTCACTGCATATGGAGCATTTTATGCAATCATGGTGTACTGCTTTATCCATgtacgtatttgcatgatcacagaccaagactttatgtattcaggtttttgtttttgtttttcaaccatgaaagatctcttgatgtctattttttggagta

upper sequence: GRMZM5G869572_T01 (Zea mays), 5'ss of exon 5
lower sequence: PP1S276_77V6.1 (Physcomitrella patens), 5'ss of exon 25
ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccccccttt--tctggagtcttttt------taagcacatcccaagagttagagaattgcatgacgaatggaacttgtttgtttggtgtg-atccag
| || || || ||  | || || ||  | ||||| |||||||| ||||| || |||||||||||||| ||||||||  | ||||||   ||| | |||| ||||||       | |    | |  ||| |||      |||| |    |  || || ||||   | | |  |    | || |||||      || |  |||   
ATAGTGCAATTACCTTAGGAAGACTGGGATGGGTTTGTCCAGAGATCGTTGCTCCTCATATGGAGCACTTCATGCAGACCTGGTGCCGTGCGCTTTGCACgtaaggatttgattgtaactttgcttctctttctgtagtaagaatcctcttagtgtcagag--ctacctagctggagcaagttgttcaagatgtatttatct--

Mapped EST sequences

Showing partial alignments of ESTs and genomic sequences. See full alignments


 ATGC     EST sequence
 ATGC     genomic sequence (exon)
 ATGC     genomic sequence (truncated intron)


EST: gi|211163358|gb|FK967901.1|FK967901
EST:     AAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
genomic: AAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
EST: gi|31363389|gb|CD447746.1|CD447746
EST:     GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
genomic: GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
EST: gi|89250982|gb|DY622768.1|DY622768
EST:     GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
genomic: GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
EST: gi|6918339|gb|AW399869.1|AW399869
EST:     CCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGACTGTGTGCAAT
genomic: CCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
EST: gi|211163359|gb|FK967902.1|FK967902
EST:     GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
genomic: GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
EST: gi|6918594|gb|AW400124.1|AW400124
EST:     CCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGACTGTGTGCAAT
genomic: CCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
EST: gi|60338787|gb|DN205760.1|DN205760
EST:     GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCAT                         GATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT
genomic: GCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAAT

RNA-Seq data


 ATGC   Supported by RNA-Seq exonic block that spans over the splice site

 atgs   Truncated intron sequence

Each block is as long as the maximal overhang of any read spanning the junction.
The score is the number of alignments spanning the junction.


Found data for 4 RNA-Seq libraries.


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 108

ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAATG


Block sizes: 47 (upstream exon), 48 (downstream exon)
Score: 71

ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAATG


Block sizes: 49 (upstream exon), 47 (downstream exon)
Score: 73

ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAATG


Block sizes: 49 (upstream exon), 48 (downstream exon)
Score: 57

ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccc ... tgtgatccagGATCAGAGATGATTTTGAGAAAGAGGACTCGTTTCATGGATTGTGTGCAATG


Putative cis-regulatory sequences

 atgc intron ATGC exonic elements by Pertea et al.
 ATGC exon atgc putative intronic elements
 ATGC putative exonic elements identified for retained introns
        10        20        30        40        50        60        70        80        90        100       110       120       130       140       150       160       170       180       190       200       210       220 
---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------|---------| 
ACAGCGCGATCACTCTTGGGAGGCTTAGCTGGGTGTGTCCAGACATCGTGGCCCCTCATATGGAGCATTTCATGCAAGCATGGTGCAAGGCGTTGTGCATgtaagggccccccttttctggagtcttttttaagcacatcccaagagttagagaattgcatgacgaatggaacttgtttgtttggtgtgatccag

- - - - - - - - - - - - - - - - - - - - - - - - - - - - - -  GGAGCA